蛋白

Aim To investigate the physiological role of Battenin interactive protein and screen the proteins interacting with BIP in human fetal cDNA library.

目的寻找Battenin相互作用蛋白的筛选方法，并对筛选出的Battenin相互作用蛋白进行初步鉴定。

Although marmoset bip is high homologous to human slam, they have some important differences.

最终证实：绒猴B-类淋巴母细胞表面存在的BIP蛋白具有与人的SLAM蛋白相同的功能，它是B95a型病毒感染B95a细胞的受体分子，虽然这两个基因具有高度的同源性（91％），但仍具有许多重要的差异。

There is close relation between BiP protein expression and increased functions of splenic macrophages induced by mild heat stress.

轻度热应激脾脏巨噬细胞BiP mRNA、BiP蛋白的表达与巨噬细胞功能发生同步改变，提示BiP蛋白表达上调可能与轻度热应激脾脏巨噬细胞功能增强密切相关。

There had a difference between biuret menthod and Folinlowry.ConclusionThe method in this paper can be used for determining protein which are rich in drugs and food.

结论所采用方法可作为蛋白含量比较高的食品、药品制剂中蛋白含量的测定。

The equation of rapid determination sesame cake protein by improving biuret method is as follows: Y=0.1559X+0.0012, the protein concentration range from 0.75 mg/mL to 3.78 mg/mL displayed better linear with absorption value.

改良双缩脲法快速测定饼粕蛋白提取率的方程为：Y=0.1559X+0.0012，蛋白浓度在0.75~3.78mg/mL之间与吸光度值成良好线性。

Methods HPDLFs were primary cultured from tissue explants, and the cells of the 5th to 8th passages were used after immunohistochemical identification of keratin and vimentin expressions. The cells were divided into 5 groups and treated with TP at 1, 0.5, 0.25, 0.125, and 0.0625 mg/ml, respectively, with another group without TP treatment as the blank control group. Cell counting and MTT colorimetric assay were performed to assess the cell proliferation, and flow cytometry was employed to determine the DNA content of the HPDLFs.

采用组织块培养法培养原代人牙周膜成纤维细胞并传代，经免疫组化SABC法检测角蛋白和波形丝蛋白鉴定，取5~8代细胞用于实验；按茶多酚不同浓度分1mg/ml、0.5mg/ml、0.25mg/ml、0.125mg/ml、0.0625mg/ml组和空白对照组，采用细胞计数法、MTT法检测细胞增殖情况，流式细胞术检测细胞DNA含量。

We studied the optimizaiton, accuracy and reliability of this technique by definition the genotype of five common mutation loci of β-globin gene in the carrier with CD41-42 mutation and that of β-globin gene of normal people's single lymphocyte and single blastomere.

我们采用了一种利用单细胞标本通过二重巢式PCR并结合ARMS技术确定中国人群中广泛的β-地中海贫血突变谱的基因型的策略，通过对含有β-珠蛋白基因CD41-42突变的携带者5个该基因常见突变位点基因型的确定，以及正常人的单个淋巴细胞、单卵裂球的β-珠蛋白基因该5个位点基因型的确定，对这种技术条件的优化、准确性和可靠性进行了研究。

Experiments on decalcification and activation of osteoinductive ability, comparison between the osteogenesis of demineralized bone matrix in orthotopic and in heterotopic sites, and correlation between immune response to allograft of bone and osteoinductive activity of the graft, were reported in this paper. 1. Syngeneic deminerolized bone matrix and the mixture of DBM with bone ash or acid-soluble protein extracted from demineralization was implanted into muscle pouch of mice, subsequently, the inhibition of DBM osteoinduction by bone ash or acid-soluble protein was demonstrated with histological and by means of alkaline phosphatase assay.

本文对脱钙处理使骨诱导活性表达的作用、脱钙骨基质植入不同部位的成骨表现，以及同种异体骨移植引发的免疫反应与骨诱导活性的内在关系三方面进行了研究：（1）在小鼠肌肉植入同系脱钙骨基质及其与脱钙所去除的骨矿盐和酸溶性蛋白混合物，碱性磷酸酶和组织学检查表明：骨矿盐和酸溶性蛋白具有抑制脱钙骨基质骨诱导活性的作用。

Results:Monoclonal antibodies IH3,5A12,7E5,7F9 showed specific respo nse to NIH3T3-erbB2 cell and SKBR3 cell,no response to NIH3T3 cell.1H3 also boun d specifically to the extracellular domains of P185 protein that expressed by E.

结果：单克隆抗体1H3、5A12、7E5、7F9可与NIH3T3-erbB2细胞、SKBR3细胞特异性结合，与NIH3T3细胞无反应；1H3与原核表达的c-erbB2胞外区蛋白有特异性反应，而与无关蛋白KDR无反应。

This method could also be used to detect bovine serum albumin and egg albumin with satisfactory results.

此方法还可应用于牛血清白蛋白、卵清白蛋白等蛋白质的测定。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。