蛋白

It is pointed out that chaperonin facilitates the correct refolding of recombinant protein and in turn increases the soluble protein.

分子伴侣蛋白帮助了重组蛋白在菌体内的正确折叠，提高了目的蛋白的可溶性。

Results Method I of gas chromatography can't be used for determination of monosaccharide in spirulina glucoprotein, while method 2 was suitable, and 7 monosaccharides were found in ion chromatography.

结果 气相色谱法1不适于藻糖蛋白单糖分析，气相色谱法2较适合藻糖蛋白单糖分析；离子色谱法测得藻糖蛋白含7种单糖。

Von willebrand factor is one glycosylated protein synthesized by blood vessel endothelial cell.Thrombomodulin is one glucoprotein which exists in blood vessel endothelial cell surface.Both of their levels are increased obviously when endotheliocyte is damaged,so they could be considered as the molecular markers reflecting endothelial injury.

血管性假血友病因子是血管内皮细胞合成的一种糖基化蛋白，血栓调节蛋白是位于血管内皮细胞表面的糖蛋白，内皮细胞损伤时，二者水平均显著升高，因此它们作为反映内皮损伤的分子标记物。

Methods: According to their urinary albumin excretion rate, Diabetes mellitus patients with micrangium disease were further divided into three subgroups: normo-albuminuria、micro-albuminuria and clinical proteinuria; The expression of platelet membrane α-glucoprotein (CD62p) and cytolysosome protein (CD63)、platelet aggregation function and mean platelet volume was determined with flow cytometer、blood aggregation analyzer、automated blood cell counter (MEK-6318K) respectively, and compared with 30 normal controls.

对糖尿病微血管病变患者按尿清蛋白排泄率分为正常清蛋白尿组、微量清蛋白尿组、临床清蛋白尿组三组，分别用流式细胞术、血液聚集仪、全自动血细胞分析仪测定血小板内α-颗粒膜蛋白(CD62p)、溶酶体蛋白(CD63)的表达及血小板聚集功能和平均体积，并与30例正常对照组比较。

Methods Lewis rats were immunized with the protein extracted from electric organ of Narcine timilei, the clinical manifestations were observed every day, and repetitive nerve stimulation,single fiber electromyography and the levels of acetylcholine receptor antibody were detected on the 49th days post immunization. The content of the protein extracted from electric organ of Narcine timile was 35.4 mg/mL, and the protein could respond with AChRAb from the serum of seropositive myasthenia gravis patient.

参照徐氏法从丁氏双鳍电鳐的电器官提取AChR蛋白，并采用Folin-酚试剂法及酶联免疫吸附法检测提取蛋白的含量及活性；以提取的蛋白主动免疫Lewis大鼠，每天观察其临床表现，并于免疫后7周进行低频重复电刺激、单纤维肌电图及血清中AChRAb水平的检测。

Methods Lewis rats were immunized with the protein extracted from electric organ of Narcine timilei, the clinical manifestations were observed every day, and repetitive nerve stimulation, ingle fiber electromyography and the levels of acetylcholine receptor antibody were detected on the 49th days post immunization. The content of the protein extracted from electric organ of Narcine timile was 35.4 mg/mL, and the protein could respond with AChRAb from the serum of seropositive myasthenia gravis patient.

参照徐氏法从丁氏双鳍电鳐的电器官提取AChR蛋白，并采用Folin-酚试剂法及酶联免疫吸附法检测提取蛋白的含量及活性；以提取的蛋白主动免疫Lewis大鼠，每天观察其临床表现，并于免疫后7周进行低频重复电刺激、单纤维肌电图及血清中AChRAb水平的检测。

The Pncps-protein conjugants prepared were helpful to promote the transfer of the classes of antibody to Pncps from IgM to IgG in serum. Under our experimental conditions, when the usage of Pncps was identical in the conjugants, the more protein conjugated, the more IgG titers to Pncps and to protein were observed.

本实验制备的荚膜多糖－蛋白的结合物中，相同多糖用量情况下，蛋白用量的增加能够促进血清中抗多糖抗体类型由IgM向IgG转变，分别提高结合物抗多糖、抗蛋白IgG抗体滴度。

Methods Physiological and biochemical parameters of the blood were determined to detect liver function. Membrane protein and nuclear protein were isolated from the liver tissue of BDL rats.

胆总管结扎术法制备大鼠梗阻性黄疸模型，检测肝功能；提取大鼠肝细胞膜蛋白与核蛋白，测定提取液蛋白浓度；采用Western blot技术检测MRP3和Lrh-1蛋白表达。

Methods Physiological and biochemical parameters of the blood were determined to detect liver function. membrane protein and nuclear protein were isolated from the liver tissue of BDL rats. The protein expressions of MRP2 and FXR were determined by Western blot.

用胆总管结扎术方法制备大鼠梗阻性黄疸模型，检测肝功能；提取大鼠肝细胞膜蛋白与核蛋白，测定提取液蛋白浓度；采用Western blot技术检测MRP2和FXR蛋白表达。

As estimated by SOS-PAGE the molecular weight of PG was 31500, lower than that of PG in other fruits. The most pH stability was at a range of pH 4.5-8.0. The most optimum ion strength was at 0.O4mol/L-0.O6mol/L. The U.V. spectrum of PG was about 267 nm.

经SDS-PAGE测得枣PG蛋白的分子量是31500，比其它果实中的PG蛋白的分子量小；PG蛋白的pH稳定范围是4.5-8.0；最适离子强度范围是0.04 mol/L-0.06mol/L；PG酶的最大紫外吸收光谱是267nm。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。