蛋白

Yeast two hybrid system was employed to screen a human T cell cDNA library for binding protein of CD2 cytoplasmic domain by using a 'bait'cDNA encoding the cytoplasmic domain of CD2 (Thr 211Glu336) amplified from human peripheral blood lymphocytes .

建立了酵母双杂交系统，以CD2分子胞内区为钓饵，筛选了人T淋巴细胞KT3 cDNA文库，获得了一个与CD2胞内区结合的胞内蛋白，命名为CD2cBP，此蛋白与v-fos转化效应蛋白（Fte-1）同源。

Results of non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis of the supernatant and aggregate precipitate formed in refolding process show that except being refolded to native egg white lysozymes, the reduced-denatured lysozymes can also form the aggregates with molecular weights being separately about 30.0 and 35.0 kD, while the reducing SDS-PAGE and the refolding results in the presence of sodium dodecyl sulphate show that these aggregates are formed chiefly through the misconnection of disulfide bonds between the reduced-denatured lysozymes, and the aggregate precipitates are formed through the non-covalent interactions between the aggregates with molecular weight being about 30.0 kD.

复性过程形成的上清和集聚体沉淀的非还原电泳结果表明，除了能复性成天然态的溶菌酶分子外，还原变性蛋白溶菌酶同时还能形成分子量分别约为30.0KD和35.0KD的蛋白溶菌酶分子集聚体；而它们的还原电泳和在SDS存在时的复性结果表明，这些集聚体主要是通过还原变性蛋白溶菌酶分子间的二硫键错配而形成，而集聚体沉淀则是通过分子量约为30.0KD的集聚体分子之间的非共价相互作用而形成。

Northern-blot analysis demonstrated that the two rice NAP1-like genes are predominantly expressed in stem tissues such as root and shoot apical meristems as well as in young flowers.

我们通过筛选单子叶植物水稻和双子叶植物烟草的cDNA文库，获得了6个NAP1类似蛋白（NAP1 like protein，NAP1_LP）的cDNA，其中水稻的两个NAP1类似蛋白分别命名为OsNAP1_L1和OsNAP1_L2，烟草的4个类似蛋白分别命名为NtNAP1_L1、NtNAP1_L2、NtNAP1_L3和NtNAP1_L4。

B. Monoclonal antibodies such as anti Fibronectin and Laminin were used to investigate the expression of structure protein.

B。应用抗纤维连接蛋白和层粘连蛋白的单克隆抗体染色观察血管壁中结构蛋白表达。

Woodchucks . The HBV genome has four genes: pol, env, pre-core and X that respectively encode the viral DNA-polymerase, envelope protein, pre-core protein (which is processed to viral capsid) and protein X. The function of protein X is not clear but it may be involved in the activation of host cell genes and the development of cancer.

乙型肝炎病毒基因组有四个基因：波兰，环境，前核心和X ，分别编码病毒DNA聚合酶，包膜蛋白，前核心蛋白和蛋白质十大功能蛋白X是尚不清楚，但它可能参与激活宿主细胞的基因和癌的发展。

In recent years, some derived yeast two-hybrid systems have been established, including yeast two-hybrid dual bait system, reverse two-hybrid system, membrane-associated two-hybrid system (SRS, TOPIS and split-ubiquitin base two-hybrid system) and transcriptional factors-associated two-hybrid system(RTA system and PolⅢ based two-hybrid system), which promise to overcome some limits and improved the sensitivities of the original yeast two-hybrid system.

十余年来，随着酵母双杂交迅速推广，不断涌现出一些衍生系统，其中包括酵母双杂交的二元诱饵系统，逆向双杂交系统，非转录读出特点的双杂交系统（如Sos蛋白招募系统、PI3K介导的靶蛋白识别系统和断裂-泛素为基础的双杂交系统）以及转录激活因子与其相关蛋白之间的相互作用的双杂交系统（如以polⅢ为基础的杂交系统和RTA系统）等。

1、Cur inhibits K562 cells growth and induces cell apoptosis may be correlated with the down-regulation of p210~、inhibition of protein tyrosine phosphorylation and the signaling molecules such as p-Erk1/2、c-myc which are relevant with cell growth and apoptosis; 2、Cur synergizes STI571 to inhibit K562 cell growth and induce cell apoptosis may be correlated with the down-regulation of p210~、inhibition of protein tyrosine phosphorylation and the signaling molecules such as Hsp90、PKC which are relevant with cell growth and apoptosis; 3、Cur reverses the resistance of K562/G01 cells to STI571, and synergizes STI571 to inhibit K562/G01 cell growth and induce cell apoptosis; 4、Cur inhibits human originated CML CD34~+ cell growth、induces cell apoptosis, and enhances STI571 to down-regulate the expression of p210~, finally inhibit cell growth and induce cell apoptosis.

从以上实验结果我们得出如下结论： 1、Cur抑制K562细胞增殖、诱导细胞凋亡的作用可能与其下调p210~、蛋白酪氨酸磷酸化水平以及抑制下游p-Erk1/2、c-myc等信号分子有关； 2、Cur协同STI571抑制K562细胞增殖、诱导细胞凋亡的作用可能与其下调p210~、蛋白酪氨酸磷酸化水平以及抑制Hsp90和下游PKC等信号分子有关； 3、Cur可逆转K562/G01细胞对STI571的耐药性，并与STI571协同抑制K562/G01细胞增殖和诱导凋亡，其抑制K562/G01细胞增殖、诱导细胞凋亡的作用可能与其下调p210~、蛋白酪氨酸磷酸化水平以及抑制下游Procaspase-3和NF-κB等信号分子有关； 4、Cur可抑制来源于CML患者骨髓的CD34~+细胞的增殖并诱导其凋亡，还可协同STI571下调CML CD34~+细胞p210~表达，进而协同抑制细胞增殖、诱导细胞凋亡。

Natural high-quality granite rollers with acid resistance, corrosion resistance, wear-resistant, non-and material from a chemical reaction, in the grinding process, such as natural cooling characteristics; granite roll grinder for grinding paint art, paint, ink, medicine, chemical industry, Date use of cosmetics and plant protein drinks (such as: almond Lu, peanut milk, pumpkin Lu), etc., especially in high-quality natural granite grinder grinding plant protein drink production with no waste, non-precipitation, not±, high raw material utilization ( is over 98%) and other unique features of plant protein drinks are the preferred manufacturer of grinding equipment.

天然优质花岗岩石辊具有耐酸碱、耐腐蚀、耐磨损、不和物料起化学反应、在研磨过程中自然冷却等特点；花岗岩石辊研磨机适用于研磨美术颜料、油漆、油墨、医药化工、日用化妆品及植物蛋白饮料（如：杏仁露、花生奶、瓜籽露）等，特别是天然优质花岗研磨机研磨生产的植物蛋白饮料具有无渣滓、不沉淀、不变味、原料利用率高（达98%以上）等独有特点，是植物蛋白饮料生产厂家首选的研磨设备。

II. Exploring non-radioactivc method to select the optimal DNA-bindingsequencc fOr singIe-chain reprcssorCloning and expression of single-chain repressors with cysteine tai1 wereperformed and immobilized these protein in the solid surface by using the standardmicrowell plates Which have been activated to contain maleimide grouPs, al1owing tobind sulthydryl-containing protein on its surface.

二。非同位素的方法筛选单链阻遏蛋白的最佳DNA结合序列初探克隆和表达了带半眈氨酸尾的单链阻遏蛋白，利用己包彼了马来酚胺的活性板可以与自由琉基结合的特性，将蛋白固定在活性板表面。

He analysis of the category and function of TCB stress inducible proteins showed that different groups of proteins were induced by 5 mg L^(-1) TCB stress. They are detoxification enzymes (including esterase, aldo/keto reductases, and glutathione S-transferase), cell wall compound metabolism related enzymes (including UDP-glucose protein transglucosylase and GDP-mannose 3,5-epimerase 1), phytohormone metabolism and regulation related enzymes or proteins (including aci-reductone dioxygenase 4, beta-glucosidase, two members of pathogenesis-related proteins from family 10), primary and secondary metabolism regulative enzymes (including translational elongation factor Tu, cytosolic orthophosphate dikinase, triosephosphate isomerases, alanine aminotransferase, and isoflavone reductase).

CB胁迫能诱导根系内不同类型蛋白质的表达，它们是：解毒酶（包括脂酶、醛／酮还原酶、谷胱甘肽－硫转移酶），细胞壁物质代谢相关酶（包括蛋白转葡萄糖基酶、GDP-甘露糖－3，5-异构酶1），激素代谢或调节相关酶（包括酸式－还原酮二加氧酶、β-葡萄糖苷酶、病程相关蛋白家族10中的2个不同蛋白），原初或次生代谢相关酶（包括转录延长因子、细胞质磷酸丙酮酸双激酶、磷酸丙糖异构酶、丙氨酸氨基转移酶和异黄酮还原酶）。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。