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The expression amount of the target protein is improved by three to ten times according to different target protein, different multiplicities of infection, different protein harvesting times and different consumptions of culture medium.

依不同目的蛋白,不同感染复数,不同蛋白收获时间及不同培养基使用量,目的蛋白表达量增加3~10倍。

BACKGROUND: Protein kinase A signaling has long been known to play an important role in cardiac function. Dysregulation of the protein kinase A system, caused by mutation of the protein kinase A regulatory subunit gene PRKAR1A, causes the inherited tumor syndrome Carney complex, which includes cardiac myxomas as one of its cardinal features.

背景:长期以来,蛋白激酶A信号途径被认为在心功能中起着重要作用,蛋白激酶A调节亚单位基因PRKAR1A的突变导致蛋白激酶A系统失调,引起遗传性肿瘤综合征-Carney合成,其中心脏粘液瘤作为其主要特征。

Background— Protein kinase A signaling has long been known to play an important role in cardiac function. Dysregulation of the protein kinase A system, caused by mutation of the protein kinase A regulatory subunit gene PRKAR1A, causes the inherited tumor syndrome Carney complex, which includes cardiac myxomas as one of its cardinal features. Mouse models of this genetic defect have been unsatisfactory because homozygote null animals die early in development and heterozygotes do not exhibit a cardiac phenotype.

背景——蛋白激酶A信号传导在心功能中发挥了重要作用早已为人们所知蛋白激酶A调节亚基因PRKAR1A的变化引起蛋白激酶A系统失调,导致了遗传性卡尼复合肿瘤综合症,其中包括的心脏粘液瘤作为它的一个主要特征此种遗传缺陷的小鼠模式仍然不能令人满意,因其纯合体空白动物在发展中早期死亡,而杂合体不能显示心脏表型

Further study demonstrated that peroxynitrite also induced tau nitration in neuroblastoma N2a cell line, and the nitrated tau was accumulated in the cells.

进一步研究发现:用PN处理稳定表达tau蛋白的N2a细胞,可以诱导细胞内tau蛋白的硝基化,而且硝基化的tau蛋白在细胞内发生聚积。

On the temperature stability, Trx-TGase could be stored in 4℃ with no degradation and no activity loss. When stored in 37℃, Trx-TGase were easily degraded but the activity of polymerizing proteins seemed not to be decreased.

在温度稳定性方面,融合蛋白于4℃保存一周相当稳定,活性几乎无损失,在37℃时,虽然融合蛋白逐渐降解为TGase单体,但其交联蛋白的活性并没有降低。

First, with the gelation property of alginate molecule in Ca2+ solution, collagen/alginate threads were prepared through thermally controlled syringing collagen/alginate (w/w=1:3) solution into 2% CaCl2 solution.

第一阶段利用褐藻酸高分子在钙离子水溶液的环境下迅速成胶的特性,再运用湿式纺织技术,将胶原蛋白与褐藻酸混合溶液(胶原蛋白重:褐藻酸重=1:3)经由恒温注射针筒系统以2ml/min的流速挤压至氯化钙水溶液(2%)的成型槽中,并控制罗拉转速,制备胶原蛋白/褐藻酸细丝。

Compare the 2-DE maps between IgA nephropathy and normal rat, 664 protein spots in 1 279 samples were matched, and 263 proteins were with over two-times high difference in the matched spots, 3 proteins were not expressed and 1 protein was uniquely expressed in IgA nephropathy.

比较IgA肾病和正常小鼠肾组织的2-DE图,在1 267个蛋白点中有664个匹配蛋白点,其中表达量差异在2倍以上的有263个,另有3种蛋白在IgA肾病肾组织中未被表达和1种蛋白特异表达。

Sheep tibetan antelope by wool horn albumen the composition of amino acid and goat, sheep by wool fibre identical; but there is certain difference on the content scale of all sorts of amino acid, acid content of ammonia of group of cashmere tibetan antelope is 5.88%, all the acid content of ammonia of content; guang of acid of ammonia of the group in prep above cashmere wool and continous abb is 10.60%, alkalescent and amino acid and acidit

藏羚羊被毛角蛋白氨基酸的组成和山羊、绵羊被毛纤维相同;但在各种氨基酸的含量比例上有一定的差异,藏羚羊绒组氨酸含量为5.88%,均高于山羊绒和绵羊毛中组氨酸的含量;胱氨酸含量为10.60%,高于其它所测山羊的绒和毛;碱性氨基酸和酸性氨基酸含量接近(14.87%,15.23%);藏羚羊绒促螺旋蛋白含量低于降螺旋蛋白

Relations between optical properties,rheological properties of Soybean Protein Isolates/soybean 7 S globulin gels and solvent,bovine serum albumin,fatty acid salts concentrations have been studied thoroughly in this thesis.Mechanisms of molecular forces to soybean protein transparent gels are investigated.

通过对大豆分离蛋白/大豆7S球蛋白凝胶光学和流变学性质与溶剂、牛血清白蛋白、脂肪酸盐浓度关系的深入研究,探讨了分子力对大豆蛋白透明凝胶的作用机理。

Protein ; Amino acid ; Albumin ; Globulin ; Glutelin ; Prolamine

蛋白质;氨基酸;清蛋白;球蛋白;醇溶谷蛋白;谷蛋白

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The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作:对伸臂位于顶部的单层框架—筒体模型进行分析,提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。

Its advantage is that it can be used in smaller units.

其优点在于可以在较小的单位中应用。