蛋白

Further improvement in antibody preparation and enrichment of protein concentration will be untaken in the near future.

许多非特异性反应的蛋白出现可能与所用的抗体纯度不够有关，因此，我们将进一步纯化此MJD多株抗体，再者，亦有可能是神经细胞所表达的MJD蛋白量太少，所以反应太弱无法被测定出来，因此，将MJD蛋白表达量增加也是未来改进的方法之一。

Methods: The urine protein and microalbumin uria of the controls and patients with diabetes and hypertension were examined with dry chemical method. Meanwhile, these results were compared with those examined with Hitachi 7600 chemical analyzer.

干化学法检测对照组、糖尿病组和高血压组尿蛋白与尿微量白蛋白，并与全自动生化分析仪检测24小时尿微量白蛋白进行比较。

The extraction procedure of crude protein from Vallisneria spiralis L. was to extract the crude protein with sodium hydroxide followed by precipitation with acid. Factors influencing the extraction rate were studied according to the experiments of odd factorsNaOH concentration,Vallisneria spiralis L.

采用碱提酸沉法从苦草中提取粗蛋白，通过单因子试验（单因素：NaOH溶液浓度、固液比、提取温度、提取时间）和L9（34）正交试验，对影响粗蛋白的提取工艺进行了研究；同时比较了不同pH值对粗蛋白沉淀的影响。

The apparent permeability coefficient of FITC-albumin was calculated using the equation Pa=(1/△If)〓(r/2), where,△If is the initial step increase in fluorescent intensity,〓 is the initial rate of gradual increase in intensity as solutes diffuse out of the vessel into the extravascular space, and r is the venular radius.

荧光标记白蛋白在微血管的通透系数则通过公式Pa=(1／△If〓(r／2)，其中△If代表了初始荧光标记白蛋白浓度，〓则是荧光蛋白渗出管壁的速度，而r是血管半径。

The molecular weights of the two subunits were shown 142 KD for the larger one and 47 KD for the smaller one, respectively, on SDS—polyacrylamid electrophoresis. The molecular weight of the native vitellin was determined as 205 KD on native gradient polyacrylamid gel electrophoresis.

用蒸馏水沉淀法从东方粘虫Mythimna seperata新鲜卵中提取卵黄蛋白，SDS-PAGE分析结果显示，粘虫的卵黄蛋白由大小两个亚基组成，大亚基分子量为142KD，小亚基分子量为47KD，线性聚丙烯酰胺梯度凝胶电泳结果显示，卵黄蛋白的天然分子量为204KD。

Results The FHIT protein is located in the cytoplasm or nuclear of normal vulvar squamous epithelium....

结果：FHIT蛋白定位于正常外阴鳞状上皮细胞的细胞浆或细胞核内。60例外阴癌中，13例(21.7％)FHIT蛋白表达呈阳性，58例(96.7％)EGF蛋白表达阳性。

Tobacco mosaic virus, a member of alphavirus-like superfamily, the N-terminal truncated TMV helicase formed hexamer and exhibited double strand RNA unwinding activity. Accordingly, we also constructed the same N-terminal truncated BaMV helicase-like domain and analysis the capability to unwind double-strand RNA.

在同属於alphavirus-like superfamily的Tobacco mosaic virus之解螺旋蛋白的分析中，其N端的截断蛋白会形成六聚体，而且具有解旋活性，因此我们同样建构了许多类解螺旋酵素N端的截断蛋白，测试其蛋白质的聚集形式及解旋活性。

Histone H3 is one of the four histones, along with H2A, H2B, and H4, which form the eukaryotic nucleosome octamer core. In this study, a new gene RH3.2A encoding an H3.2-type histone H3 protein from rice (Oryza sativa L.) was reported. RH3.2A was cloned through RT-PCR from salt-treated rice seedlings.

本研究从盐胁迫处理的水稻幼苗组织中分离了一个新的水稻组蛋白H3基因RH3.2A，编码具有136个氨基酸残基的多肽，与多种植物的组蛋白H3蛋白具有高度的氨基酸一致性。

The dynamic changes of the the neuron apoptosis and correlative genes in hippocampus of ACET-groups and twice-ACET-groups suggested that ACET can slower the apoptosis by inhibiting p~(53)and prolong the ascending period of p~(53) in 4 days after the seizure,this effect would weaken after 4 days.ACET may inhibit the descending of Bax,prolong its descending period and then promote its expression,in order to weaken the inhibition of the apoptosis during 4 to 10 days after seizure and then promote the apoptosis during 10 to 14 days after.This effect of ACET can be enhanced after treating again within 7 to 14 days later.

埋线组和二次埋线组大鼠海马神经元凋亡及相关基因表达的动态变化结果提示：埋线治疗在癫痫发作后的早期(4d内)可通过抑制促凋亡基因p~(53)蛋白表达、延缓其达峰值的时程，从而减缓神经元凋亡的发生，此作用在4d后减弱；埋线治疗抑制大鼠海马神经元凋亡的作用在癫痫发作后的中期(4d～10d)减弱，甚至在远期(10d～14d)出现促进凋亡的作用，可能是通过抑制Bax蛋白表达的下降、延长其降至低峰值的时程，促进Bax蛋白表达的回升的结果；在中远期(7d～14d)内予二次埋线治疗对该效应有续效叠加的作用。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。