蛋白

For example, it is often presumed that cardiac ascites is a transudate when this is rarely the case, ascitic protein is .25 g/l in up to 30% of patients with otherwise uncomplicated cirrhosis,32 40–43 and patients with cirrhosis and tuberculous ascites may have a low ascitic protein.44 The serum ascites-albumin gradient is far superior in categorising ascites with 97% accuracy (table 1).42 45 46 It is calculated as

如通常假定心源性腹水为漏出液而这其实非常少见，在达30％的没有并发症的肝硬化病人中腹水蛋白＞25g/L，32，40-43有肝硬化和结核腹水的病人也可能腹水蛋白很低。44血清腹水-白蛋白梯度在腹水分类时有更高的优越性，准确率达97%（表1）。42，45-46计算方式如下

The 8 proteins were attributed to the processes of 26S protein dehydration, respiration, tricarboxylic acid cycle, chlorophyll synthesis, glyoxylate cycle, respectively, and of which one spot was cold resistant protein.

在这4次降温过程中重复出现的8蛋白点分别参与265蛋白水解途径、呼吸代谢、三羧酸循环、叶绿素合成，其中有一个位点是抗性蛋白。

The relationships of VEGF with the indices of renal damage, including renal/body weight, urinary protein excretion, glomerular volume and glomerular area, were analyzed. Results The expression of VEGF mRNA in diabetic kidney was significantly up-regulated after operation from 2 weeks to 24 weeks with the peak level at 20weeks, when compared with control at the same time- points. The positive results of VEGF staining in diabetic glomeruli was increasingly observed after operation from 2 weeks to 24 weeks, with the peak at 20 weeks. The positive results of VEGF staining in diabetic tubuli was increasingly seen from 2 weeks to 24 weeks, with the peak at 8 weeks.

结果 VEGF mRNA在糖尿病模型组大鼠肾脏中持续高表达，各时点与对照组相比差异均有统计学意义(P.05)；糖尿病组2~24周肾小球VEGF蛋白表达强于对照组(P.05)，24周时有所减弱，但仍强于对照组；糖尿病组各时点肾小管VEGF蛋白表达均强于对照组(p.05)， 8周后表达明显增强；VEGF表达变化与肾质量／体质量(r=0.518， P＝0.001)、尿蛋白(r=0.409， p=0.001)、肾小球面积(r=0.499， P-0.000)和体积(r=0.499， P-0.000)呈正相关。

State Key Labratory of Veterinary Biotechnology,Harbin Veterinary Research Insti tute,CAAS,Harbin150001

有关研究表明VP1是CAV 唯一的结构蛋白，VP2为辅助蛋白，与病毒的装配有关，而VP3是CAV的致病性蛋白［1］。

These genes encoded for the proteins AWP1 (associatedwith protein kinase 1) and "BTB domain containing 1,"whose functions are largely uncharacterized.

这些基因编码 AWP 1蛋白（与蛋白激酶1相关）和& BTZ domain containing 1&，这些蛋白的功能大多仍不知道。

The drop in apolipoprotein II in the long walkers was matched by a rise in the blood level of apolipoprotein I, a "good" fat that is associated with unclogged arteries.

长距离步行者载脂蛋白II的下降部分与血液中载脂蛋白I的上升部分相当，载脂蛋白I是一种与动脉疏通有关的&有益&的脂肪。

Based on the crystal complex structure of ligand and receptor proteins, we have developed several strategies for grafting the binding site of the native ligand protein to unhomologous scaffold proteins.

把蛋白-蛋白相互作用区嫁接到另外一个非同源的骨架蛋白上，有助于了解蛋白质结构和功能之间的关系，并且在医学上具有潜在的价值。

However, in the absent of LEF-2, the formation of viral nucleocapsid derived from lef-2 ko bacmid appeared to be normal under transmission electron microscope, suggesting that the life cycle of lef-2 ko bacmid could proceed through DNA replication to viral pakckaging unimpededly.

虽然病毒之复制受到lef-2基因缺失的影响，但由於病毒之构造蛋白仍可表现，如鞘蛋白(VP39)等，因此经由穿透式电子显微镜观察，可知缺失lef-2之病毒仍可有正常的核酸蛋白鞘形成。

However, the molecular mechanism is still unknown. Here the binding patterns of GATA factors (GATA-1 and GATA-2) to the regulatory elements of human β-globin gene were examined with the nuclear extracts from the HU-induced and uninduced HEL cells.

结果表明，羟基脲诱导后，与人β—珠蛋白基因5'远侧端DNaseⅠ超敏感点2核心序列以及近侧端启动子序列相结合的GATA-1蛋白因子量明显增多，而GATA-2蛋白因子量明显减少。

Additionally XBP1 mRNA which functioned as centrally regulating molecule during ER stress was spliced partially,while it was completely spliced in positive control cells and unspliced in H7721.These results gave the proof that blocking expression of GnT-V caused H7721"s ER stress and response was more weak than that caused by DTT. It may be the chronic process.Additionally,previous studies reported that the ds-RNA may activate the kinase PKR which phosphorylated eIF2 α and inhibited synthesis of proteins.In this paper the antisense cDNA of GnT-V was integrated with GnT-V-AS/H7721"s gene and functioned through the binding of antisense RNA and mRNA of GnT-V.Additionally,the integration of GnT-V cDNA in genome also may be a non-specific factor.

对这三种ER stress关键分子的检测发现：和阴性细胞相比较，实验细胞中BIP的表达无论是蛋白水平还是转录水平都有明显的上调，但上调程度都要低于DTT处理的ER stress阳性细胞；XBP-1 mRNA在实验细胞中部分被剪接，在阳性细胞中XBP-1 mRNA完全被剪接，而在阴性细胞中其以非剪接形态存在；此外和DTT处理的ER stress阳性细胞相似，实验细胞中的PERK发生磷酸化，表明ER stress过程中通过磷酸化eIF2α抑制蛋白合成机制的活化，这和芯片所检测到的GnT-V-AS／H7721细胞蛋白合成系统水平下调相一致。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。