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By the bioinformatics analyzing tools in bioinformatics webs site such as NCBI (http:///www.ncbi.nlm.nih.gov/), ExPaSy (http://www.expasy.org/) and combining else complicated bioinformatics software package ,to identify a EF-1 full-length gene from the Taenia asiatica eDNA plasmid libratory and predict the structure and function of the protein.

方法利用生物信息网站如美国国家生物技术信息中心(NCBI,http:∥www.ncbi.nlm.nih.gov/)和瑞士生物信息学研究所的蛋白分析专家系统(ExPASY,http:∥ca.expasy.org/)中有关基因和蛋白的序列和结构信息分析的各种工具,结合其它生物信息学分析软件包,从亚洲带绦虫成虫全长cDNA质粒文库中识别延伸因子-1的全长编码基因并预测其蛋白的结构和功能。

Retrospectivly analyse the coronary heart disease, 68 patients diagnosed the coronary angiography, including Unstable Angina the 24 patients, Stable Angina 32 patients, myocardial infarction 12 patients; Compares the change of normal comparison group 35 patients fasting blood lipide:Trighycerides, Total Cholesterol, High Density Lipoprotein Cholesterol, Low Density Lipoprotein Cholesterol, Apolipoproteins (ApoA1 and ApoB), Fasting Blood Glucose, Fasting Insulin, Insulin area under curve and Glucose area under curve.

回顾性分析经冠状动脉造影确诊的冠心病患者68例,其中包括不稳定型心绞痛患者24例,稳定型心绞痛32例,心肌梗塞12例;正常对照组35例的空腹血脂(血清甘油三酯TG、血总胆固醇TC、高密度脂蛋白胆固醇HDL-C、低密度脂蛋白胆固醇LDL-C、载脂蛋白ApoA1及ApoB)、空腹血糖、空腹胰岛素、糖负荷后胰岛素曲线下面积及葡萄糖曲线下面积的变化并进行比较。

METHED We selected 40 patients underwent coronary angiography. Blood samples for measurement of CRP, Cardiac Troponin I, Creatine Kinase, MB isoenzyme of CK and lipoidemia were taken in the morning just before angiography in all patients. Each subject was sought details of sex, age. Body Mass Index, history of smoking, hypertension, diabetes mellitus.

方法选取住院并接受冠状动脉造影(coronary angiography, CAG)的患者40名进入本研究,均于CAG前采集空腹血,检验C反应蛋白、肌钙蛋白Ⅰ(Troponin Ⅰ,TnⅠ)、甘油三酯、总胆固醇、高密度脂蛋白胆固醇、肌酸激酶、肌酸激酶同工酶MB,并收集所有患者年龄、年龄、高血压史、糖尿病史、吸烟史、体重指数的资料。

OR>1 indicates dangerous factor, OR<1 protective factor, and OR=1 no significance. The results showed that if triglyceride and very low density lipoprotein cholesterol increased an average level of normal respectively, and there would be OR of TG 2.43 (P<0.05) and OR of VLDL-C 6.09 (P<0.05), the high levels of TG and VLDL-C were the factors of lithogenesis. High density lipoprotein cholesterols (HDL1-C, HDL2-C, HDL3-C), with OR less than one, were the factors of protecting against the formation of gallstone.

结果:甘油三酯和极低密度脂蛋白胆固醇每增加一个正常均值水平,OR值分别是2.43(P<0.05)和6.09(P<0.05),是重要的致石因素指标;高密度脂蛋白胆固醇(HDL1-C、HDL2-C、HDL3-C),OR值均低于1,是重要的防石因素指标;低密度脂蛋白胆固醇和总胆固醇的OR值均小于1,本研究结果提示其可能是一种代谢状态而非保护因素。

The amino acid sequence similarity of C3d between human, bovine, goat, boar, golden hamste,rmouse,brown rat,Macropus, hare,Gallus is 80.4%,94.7%,96.9%,82.8%,81.1%,80.6%,80.2%,71.0%,75.2% and60.3% respectively..

通过生物学软件分析发现绵羊C3d蛋白有2个蛋白激酶C磷酸化位点,1个酪蛋白激酶Ⅱ磷酸化位点,1个酪氨酸激酶磷酸化位点,1个十四酰化位点。

And the different concentrations of natural monoclonal anti-keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti-keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

结果:与对照组比较,不同浓度的单克隆天然抗角蛋白抗体IgM3B4均能抑制白念珠菌芽管生成,并且抗体浓度越高,抑制白念珠菌芽管形成的作用越明显;同时单克隆天然抗角蛋白抗体IgM3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附,其抑制作用与单克隆天然抗角蛋白抗体IgM3B4的浓度呈正相关。

Keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein. RESULTS: It was found that the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 could inhibit Candida albicans germ tube formation and adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.Its inhibitory action was positively related to the level of natural monoclonal anti??

结果:与对照组比较,不同浓度的单克隆天然抗角蛋白抗体IgM 3B4均能抑制白念珠菌芽管生成,并且抗体浓度越高,抑制白念珠菌芽管形成的作用越明显;同时单克隆天然抗角蛋白抗体IgM 3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附,其抑制作用与单克隆天然抗角蛋白抗体IgM 3B4的浓度呈正相关。

Chicken Meal, Chicken, Brown Rice, Duck Meal, Barley, Oatmeal, Chicken Fat (Preserved With Natural Mixed Tocopherols), Potatoes, Carrots, Lamb Meal, Salmon Meal, Canola Oil, Natural Flavor , Dried Egg, Brewers Yeast, Whole Ground Flaxseed, Potassium Chloride, Choline Chloride, Taurine, Spinach, Parsley Flakes, Cranberry, Lysine, L-Carnitine, Yucca, Kelp, Zinc Proteinate, Vitamin E Supplement, Niacin, Manganese Proteinate, Copper Proteinate, Zinc Sulfate, Manganese Sulfate, Copper Sulfate, Thiamine Mononitrate (Vitamin B-1), Vitamin A Supplement, Biotin, Potassium Iodide, d-Calcium Pantothenate, Riboflavin (Vitamin B-2), Pyridoxine Hydrochloride (Vitamin B-6), Vitamin B-12 Supplement, Manganous Oxide, Sodium Selenite, Vitamin D Supplement, Folic Acid.

鸡肉粉;鸡肉,糙米,鸭肉,大麦,燕麦,鸡肉脂肪,马铃薯、胡萝卜,羊肉粉,鲑鱼粉,芥菜油,天然香料,蛋粉,啤酒酵母;卵磷脂;亚麻仔,氯化钾,氯化胆碱,菊糖;牛磺酸,菠菜,荷兰芹,蔓越莓,赖胺酸,左旋肉碱,丝兰,海带,锌蛋白,维生素E补充,菸碱酸,锰蛋白,铜蛋白,硫酸锌,硫酸锰,硫酸铜,硝酸硫胺明(维生素B1来源),维他命A,维生素H,碘化钾,D-泛酸钙,维生素B-12 补充;核黄素(维生素B-2来源),维生素D补充,叶酸。

OBJECTIVE: It is hypothesized that arrhythmia can be treated by changing Cx levels and intervening abnormal GJ channel. Moreover, to explore the effects of bone marrow mesenchyma stem cells on the expression of Cx43 and Cx45 in rats with myocardial infarction.

目的:课题组假设通过改变缝隙连接蛋白水平、干预异常的GJ通道,来尝试治疗心肌梗死后心律失常,为此探讨同种异体骨髓间充质干细胞移植对心肌梗死大鼠缝隙连接蛋白43及缝隙连接蛋白45 mRNA表达的影响。

We initiated a genetic screen for Drosophila olfactory genes by means of SG18.1-Gal4/UAS system: The result validates our screen as an rapid, effective approach for recovering genes controlling glomerular map patterning; From a misexpression screen of 1,515 P{GS} lines, we identified 23 genes that, when forcibly expressed in the olfactory receptor neurons, disrupted the stereotyped anatomy of the Drosophila antennal lobes; These genes, which have not been shown previously to control olfactory map development, encode novel proteins as well as proteins with known roles in axonal outgrowth and cytoskeletal remodeling.

本文首次利用SG18.1-Gal4/UAS基因筛选系统对果蝇嗅觉基因进行了筛选和鉴定:结果表明,所采用的方法可以快速、有效地分离到果蝇嗅觉相关基因;从1515株果蝇P{GS}品系分离到86株突变体,其中40株有明显表型,从中鉴定了23个基因,这些基因在ORN强迫表达时可以损坏果蝇嗅叶的固有结构;在筛选中发现的一些调节嗅觉图谱发育的基因可以编码新蛋白或编码参与轴突生长和细胞骨架重塑的蛋白;为了便于研究,根据基因的功能和所编码蛋白的特点,我们对分离到的基因进行了分类,即参与轴突引导和突触产生、调节转录、调节细胞骨架和功能未确定或未知的基因;并通过对嗅觉感觉器的检测发现,除了调节转录的基因外,其他基因对触觉器的数量和分布影响不明显。

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