蛋白

The properties of several rhodopsin sequences were analyzed by comparing withα2A-adrenergic receptor, and a great similarity was found between bovine rhodopsin andα2A-adrenergic receptor. Therefore transmembrane helices ofα2A-adrenergic receptor were constructed homologically based on the bovine rhodopsin.In the newly constructed structure, a 0.090 nm3 active-site, which was enclosed by several reported active residues, was found.

通过对比多个与α2A-肾上腺素受体同属G-蛋白偶联受体的视紫红质蛋白序列，选择以相似性最大的牛视紫红质蛋白为模板，同源模建了α2A-肾上腺素受体的跨膜结构，并在结构中找到了体积为0.090 nm3，已被报导的活性残基包围的活性位点。

The highest lysine content of protein component was albumins, the next was glutelins, the lo west was hordein.

各组分中的赖氨酸，以盐溶蛋白中最高，谷蛋白中次之，醇溶蛋白中最低。

According to the results of Blastn and Blastp in GenBank, it shares 72%,70%,60%,54% nucleotide sequence identity and 79%,73%,65%,57% amino acid sequence identity with Hordeum vulgare lipid transfer protein gene LTP7a2b and LTPcw-19, Zea mays ltp and Brassica napus ltp separately.

将核苷酸序列和推断的氨基酸序列在GenBank中进行比较，发现Siltp与大麦脂转移蛋白LTP7a2b和LTPcw-19，玉米脂转移蛋白，欧洲油菜的脂转移蛋白基因的核苷酸序列的同源性分别为72%，70%，60%，54

According to the results of Blastn and Blastp in GenBank, it shares 72%,70%,60%,54% nucleotide sequence identity and 79%,73%,65%,57% amino acid sequence identity with Hordeum vulgare lipid transfer protein gene LTP7a2b and LTPcw-19, Zea mays ltp and Brassica napus ltp separately.

将核苷酸序列和推断的氨基酸序列在GenBank中进行比较，发现Siltp与大麦脂转移蛋白LTP7a2b和LTPcw-19，玉米脂转移蛋白，欧洲油菜的脂转移蛋白基因的核苷酸序列的同源性分别为72%，70%，60%，54%；氨基酸序列的同源性分别为79%，73%，65%，57%。

Results Among the three groups,the children's rib cartilage had the most blood vessels,the most chondrocytes,well-distributed stain of matrixes,and the type Ⅱ collagen was expressed actively and highest in photedensity.The rib cartilage of teenager group had less blood vessels,unhomogeny distributed stain of matrixes,the enlarged and separated cartilage lacunas.The rib cartilage in adult group showed the least blood vessels,the least chondrocytes.the hyalinization of perichondium,the most deposition of calcium salt,and the type II collagen was expressed at the lowest level in photodensity.

结果 儿童组肋软骨膜血管最丰富，软骨基质染色均匀，软骨细胞数目最多，Ⅱ型胶原蛋白表达最活跃，平均积分光密度值最高；青少年组软骨膜内血管减少，软骨基质染色出现明显的不均质状，软骨陷窝体积变大，并呈分隔状，陷窝内软骨细胞数目减少，II型胶原蛋白表达较儿童组减弱；成人组软骨膜血管、细胞成分明显减少，软骨膜内的纤维成分明显玻璃样变，钙盐沉积较青少年组时明显增多，Ⅱ型胶原蛋白表达较青少年组减弱。

Secondly, although protein sequence hydropathy prediction result showed ZmDWF1 is an integrated membrane protein, we still successfully expressed the full-length protein fused into the plasmid pET30a , then purified the 70 KD fusion proteins by Ni-NTA agarose affinity chromatography column.

其次，尽管蛋白氨基酸序列的疏水性分析表明，ZmDWF1是一种整合膜蛋白，我们依然将ZmDWF1全长序列克隆到原核表达载体pET30a中，并成功的在大肠杆菌BL21（DE3）中表达了这种预测的膜蛋白全长。

Western-blot analysis and indirect immunofluorescence assay showed that the gp90 protein expressed successfully was 45ku in molecular weight,possessed good reactionogenicity and could be recognized by mouse hyperimmune sera against the gp90 protein under natural or denatured state.

抗gp90蛋白小鼠超免疫血清介导的Western-blot分析和间接免疫荧光试验结果显示，gp90蛋白被正确表达，分子质量约为45ku；gp90蛋白在变性和自然状态下均能够被识别，具有良好的生物活性。

After transfection of E.coli BL21 and induction by IPTG,the recombinant immunotoxin fusion protein was highly expressed in E.coli BL-21 in the form of inclusion bodies(up to 32.29% of total cellular proteins).

IPTG诱导后目的蛋白获得高效表达，SDS-PAGE分析目的蛋白主要以不溶性包涵体的形式存在于菌体中，表达量占菌体总蛋白的32.29％。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

The result showed that lamin B recruited to the chromosome gradually after 6-DMAP was added into the culture solution.

此外，我们采用免疫荧光染色观察PM胚胎核膜重现过程中核纤层蛋白B的动力学变化，结果显示：在加入6-DMAP后核纤层蛋白B在染色体周围逐渐聚集，约3h后核膜完全形成，此时核纤层蛋白B在染色体周围的聚集达到最高峰。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。