蛋白

The circinal cavities were found in calcium alginate beads and the cylinder holes were observed in zinc alginate beads from the SEM,which indicated that the volume of fraction of zinc alginate polymer was relatively bigger and the zinc alginate polymer chain was more rigid than that of calcium alginate .

凝胶的SEM照片可见，海藻酸钙的冷冻干燥颗粒内为较大的圆孔，海藻酸锌凝胶内为较小的长孔，表明海藻酸锌高分子链在凝胶颗粒中的体积分率相对较大同时刚性较强；卵清蛋白从海藻酸凝胶颗粒中释放的试验结果表明，由于上述海藻酸锌凝胶的特性，导致海藻酸锌对卵清蛋白扩散阻滞作用相对较强；根据试验数据计算得卵清蛋白在海藻酸钙、海藻酸锌凝胶颗粒中的扩散系数分别为1.19×10-7、0.07×10-7cm2/s，利用阻滞模型计算得海藻酸锌高分子链在凝胶颗粒中体积分率约为海藻酸钙高分子链在凝胶颗粒中体积分率的1.7倍。

Objective to evaluate the functional recovery of acute spinal cord injuried rats treated with exogenous wnt-3a signal protein administration and to explore its mechanism.methods moderate spinal cord contusion injury was made in 40 adult sprague dawley rats at t10.twenty rats served as contusion controls(group 1).twenty rats were treated with wnt-3a for three days after injury (group 2).the functional recovery of the rats was observed through basso,beattie,bresnahan open field locomotor score.rats were killed at 14 or 28 days after injury,then spinal cords were removed for histopathological examinations,and the expression of the bromodeoxyuridine plus neural cell markers was stained with immunohistochemical method.results rats of two groups receiving a contusive injury recovered substantial function within 1 week.by 28 days,rats in groups 2 scored 7.0 points better on the bbb scores than rats in group 1 group 2=16.94,after 28 days vs.

目的 研究外源性wnt-3a信号蛋白对脊髓损伤的修复作用，并探讨其作用机制。方法取40只成年雌性sd大鼠在t10节段制备适度脊髓挫伤模型。随机从中取20只为损伤对照组(group 1)，另外20只为损伤治疗组(group 2)。脊髓损伤3天后用wnt-3a蛋白治疗。这些大鼠的功能恢复通过basso、beattie、bresnahan开放视野运动评分来观察。这些大鼠分批在损伤后14天或28天被处死，取出损伤节段脊髓用来组织病理学检查，同时用5-溴脱氧尿嘧啶核苷和神经细胞标记物进行免疫组化染色。结果两组大鼠在伤后一周运动功能有明显的恢复。不过，到损伤后28天，我们观察发现，损伤治疗组中的大鼠bbb评分比损伤对照组中的评分高出7.0分左右(group 2∶16.94±1.18，group 1∶9.89±1.29；p.05)，光镜和电镜检查发现wnt-3a蛋白对髓鞘形成和轴突再生有一定的修复效果。

The hydrophobicity of amino acid residues in the α-crystallin domain of small heat shock proteins is thought to be important for polypeptide binding and play key roles in substrate protection.

低分子量热休克蛋白α-水晶体蛋白区域的胺基酸疏水性与多胜肽的键结有其重要性并扮演著保护受质蛋白的功能。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

The eliminating protein rate and loss condition of polysaccharide were compared. The effectof negative ion macroporous resin was suitable. The condition of this method was gentle, easy torealize industrication, and was of decolour effect. The eliminating protein rate was79.26％,polysaccharides obtained rate was 86.3％.

从蛋白脱除率及多糖损失情况进行了比较，用弱碱性阴离子大孔树脂脱蛋白效果理想，该法条件温和、有脱色效果、易工业化生产，蛋白脱除率达79.26％，多糖回收率达86.3％。

Double positive rate was about 80%. Albumin increasingly expressed following induction, but alpha-fetoprotein decreasingly expressed. Simultaneously, c-kit mRNA levels were significantly reduced. No significant changes were found in keratin-19 levels.

细胞诱导后肝细胞标志物白蛋白表达明显增加，而作为不成熟肝细胞的经典标志物甲胎蛋白表达减少，同时干细胞标志物 c-kit mRNA水平也明显下降，角蛋白19水平无明显变化。

Deguelin induced DNA fragmentation and apoptotic cell death in a dose-dependent manner, which was accompanied by typical morphological changes associated with apoptosis.

3鱼藤素能同时在蛋白和基因水平，以剂量依赖性方式抑制核孔蛋白Nup88、Nup214以及核磷蛋白NPM的表达。

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞，用100 TCID50柯萨奇B3病毒（CVB3）感染心肌细胞，建立病毒性心肌炎实验模型，然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞，观察心肌细胞的形态和搏动，用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白，四唑蓝比色法测定心肌细胞活性和病毒抑制率，流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

Immunocytochemical staining was used to identify sarcomeric actin and intercalated disc-like structure when the cells were cultured for additional 1 week, 2 or 3 weeks, respectively. RESULTS: Sarcomeric actin positive cells were observed in 1 week, 2 or 3 weeks after 5-aza treatment. Some cells stained positive for connexin43 at 1 week and 2 weeks after 5-aza treatment, with brown pellet located dispersively around nucleus.

结果： 5-aza处理后1周、2周、3周均可见横纹肌肌动蛋白染色阳性细胞。5-aza处理后1周及2周连结蛋白43在部分细胞上表达：呈核周散在分布的棕黄色颗粒，5-aza处理后3周连结蛋白43 在细胞密度大的区域呈核周聚集成线形分布的棕黄色颗粒，个别细胞间可见此结构，类似正常心肌的闰盘结构。

Besides, the downstream of GGPP metabolism in diterpene metabolic pathway is now still unknow.

GenBank蛋白数据库中仅见有15条丹参蛋白质序列，在这些报道的蛋白中，与丹参酮类成分代谢途径相关的酶蛋白就更少，此外，丹参酮类成分次生代谢在GGPP以下的下游代谢途径目前还未知。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。