蛋白

AIM: To observe the effect of solanine on the contents of caspase-3 and Bcl-2 in HepG2, and to explicate the mechanism by which solanine induces the apoptosis of tumor cells. METHODS: Laser confocal scanning microscopy and Western blot were used to measure the contents of caspase-3 and Bcl-2, and LCSM was used to determine their locations in the cell. RESULT: Solanine markedly increased the content of caspase-3 while decreasing that of Bcl-2 in HepG2, both in a dose-dependent way.

目的：观察龙葵碱对HepG2细胞内caspase-3及Bcl-2蛋白含量的影响，阐明龙葵碱诱导肿瘤细胞凋亡的作用机制方法：采用激光共聚焦扫描显微术和Western blot法检测caspase-3和Bcl-2蛋白含量，并对二者在细胞内的位置进行定位结果：龙葵碱能够显著升高跳西2细胞内caspase-3蛋白含量，降低Bcl-2的含量，并且均具有剂量依赖性。

Background— Numerous in itro studies suggest that sphingosine 1-phosphate (S1P), a bioactie lysosphingolipid associated with high-density lipoproteins, accounts at least partly for the potent antiinflammatory properties of high-density lipoprotein and, thereby, contributes to the antiatherogenic potential attributed to high-density lipoproteins.

背景——众多的活体研究表明鞘氨醇1-磷酸盐作为一种与高密度脂蛋白相关的生物活性溶血磷脂，至少是高密度脂蛋白有力抗炎特质的部分原因，从而促成高密度脂蛋白的抗动脉粥样硬化潜能。

The content variation to Chrysopa septempunctata"s vitellin was detected through ELISA. It indicated that vitellin was utilized gradually during embryonic development, and a fallowing trend in form of straightline was found in the whole embryonic development. Vitellin couldn"t be detected in just hatched larvae.

通过ELISA来测定大草蛉的胚胎发育和胚后发育中卵黄蛋白的含量变化，随着胚胎的发育，卵黄蛋白逐渐被利用，在整个胚胎发育过程中呈直线下降趋势，在刚孵化的幼虫中已检测不到卵黄蛋白的存在。

The apoB gene polymorphism on Xba I site of 53 6 unrelated Chinese Han people,aged 25～64 years,was analyzed using PCR and Re striction Fragment Length Polymorphismmethod;serum apoA I,apoB,total chole s terol,triglyceride,HDL cholesterolwere measured,and LDL choleste roland non-HDL cholesterolwere calculated.

采用聚 合酶链反应结合限制性片段长度多态性分析的方法，分析536名25～64岁的无血缘关系的汉族人的apoB基因Xba I位点多态性，并测定其血清apoA I、apoB、总胆固醇、甘油三酯、高密度脂蛋白胆固醇，并计算低密度脂蛋白胆固醇LDL-C）及非高密度脂蛋白胆固醇。

Only small numbers of shrunken cells were observed in Cyclin B1 over-expressing cells, while markedly morphological changes were observed in the control subline.

Cyclin B1蛋白高表达抑制细胞凋亡的可能机制是，正常表达的Cyclin B1蛋白存在于细胞质中，促进细胞增殖、抑制细胞凋亡；药物诱导的Cyclin B1蛋白存在于细胞核中，促进细胞凋亡。

Annexin 5, a calcium-dependent phospholipid binding protein, is one of the most important members in annexins superfamily.

膜联蛋白5(annexin 5)是一种钙离子依赖的磷脂结合蛋白，是膜联蛋白家族最重要的成员之一。

The core DNA sequences of DNase Ⅰ supersensitive sites might play an significant roles in regulating the expression of β-globin gene through the interaction with nuclear matrix and nuclear malrix proteins.

人β-类珠蛋白基因调控元件LCR中的DNaseⅠ超敏感点（HS2，HS3和HS4）的核心序列通过与核基质蛋白及核基质的相互作用调控下游β-珠蛋白基因的表达。

Methods Resazurin and MTT assay were used to detect the effects of scorpion tegument water-soluble protein, scorpion tegument keratin protein and STP component Ⅱ on the transformation of T and B lymphocytes, and NK cell activity in normal mice.

采用刃天青和MTT法检测蝎皮水溶性总蛋白、总角蛋白及水溶性蛋白组分Ⅱ对正常小鼠T、B淋巴细胞转化、NK细胞杀伤活性的作用。

Japonicum, covering 90% of its total genes; find 8400 protein-coding genes; set up the largest functional gene database and clone libraries in the world; for the first time, identify as many as 3260 proteins from variety of life stages of S. japonicum; analyse the eggshell proteins and tegument proteins, which were the putative targets for drugs and candidates of vaccines; reveal a large quantity of genetic polymorphisms in the genes of S.

对人CD34+造血干／祖细胞进行了转录组和蛋白质组学研究，鉴定了370个蛋白质，这是迄今为止鉴定最多的CD34+细胞蛋白，并且鉴定了各种转录组研究没能发现的133个CD34+细胞蛋白，发现了CD34+细胞具有可塑性的新的证据，证明了反义核酸未能完全阻止其相应基因的表达，并提出一些蛋白的异质性是由于翻译后修饰造成；2。

Results Same as hyaline cartilage, the calcified cartilage was stained light flavo-green and weak double refraction by the Picrosirius-polarization method. In the immunohistochemistry analysis of collagen Ⅱ protein, the calcified cartilage was stained strong green fluorescence and the collagen protein parallelled in joint surface near the tidemark. The content of collagen Ⅱ protein was (20.16%±0.96%) of tissue dry weight by amino acid analysis, and that of the hydroxyapatite was (65.09%±2.31%) of the inorganic composition in calcified cartilage.

结果 天狼猩红染色见钙化软骨层与透明软骨层一样黄绿色淡染、弱双折光性；Ⅱ型胶原蛋白免疫组织化学分析，钙化软骨层呈强绿色荧光，潮线附近的胶原蛋白呈板层平行分布；氨基酸定量分析钙化软骨层Ⅱ型胶原蛋白含量占组织干质量的（20.16%〖KG-*4〗±0.96%）；X-射线衍射定量分析钙化软骨层中的羟基磷灰石含量占无机成分的（65.09%±2.31%）。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。