蛋白

The effect of TATp53 on the growth of parasites was determined by counting the parasitemia changes between pre and postincubation of the fusion protein with the parasite culture for 12 h. RESULTS: TATp53 fusion protein could be transduced into P.f parasites, locating at trophozoit and lateschizont stage, whereas p53 protein could not.

结果：TATp53融合蛋白可以突破红内期疟原虫各层细胞膜，进入虫体细胞内，并可根据荧光观察到融合蛋白主要分布在疟原虫滋养体和晚期裂殖体，而p53蛋白不能进入疟原虫内。

Parasite nucleic acid vaccine,a new type of vaccine emerging and developing in recent years,can induce not only humoral immunity,but also a high level of cellular immune responses.Parasite DNA vaccine displayed a great advantage in the prevention of parasitosis.

核酸疫苗(nueleie aeid vaeeine，DNA vaeeine)，也常被称为基因疫苗是指将编码某种抗原蛋白的外源基因直接导人动物细胞内，并通过宿主细胞的表达系统合成抗原蛋白，诱导宿主产生对该抗原蛋白的免疫应答，以达到预防和治疗疾病的目的。

The changes of nutrition in milk treated with different technologies are also determined. Results showed that, 1 higher pressure, longer holding time and more alternative times favour a larger destruction of microorganisms; 2 the combined effect of hurdle technology and pressure on the destruction of microorganisms in fresh raw milk is more effective than only using pressure treatment, among which the effect of ε-polylysine on inactivation of microorganism in fresh raw milk is more effective than that of Nisin with the same concentration of 100mg/L; 3 the whole proteins in milk treated by the different types of sterilization have been detected by SDS-PAGE electrophoresis, and comparing with the whole proteins' electrophoretogram of fresh raw milk, we found that the first spectrum band near the negative pole was disappeared and the colours of the second spectrum band was tint in UHT, pasteurism and 500MPa treated milks, which was suggested that some proteins' subunits were dissociated, but the 400MPa treated milk showed the similar eletrophoretogram with the fresh raw milk; 4 the loss of threonine, VA and VC in high pressure treated milk was more less than those in pasteurism treated milk, in particular threonine and VA were almost retained, and the contents of VB1 and VB2 in the former are also more than that in the latter; 5 high pressure treated milk had longer shelf life than pasteurism treated milk, extending the sale semidiameter of milk.

压力越高，保压时间越长，交变次数越多，杀菌效果越好；2）采用栅栏技术对牛乳中的微生物具有更有效的杀菌作用，其中相同浓度的（终浓度100mg/L）ε-聚赖氨酸较Nisin具有更强的杀菌效果；3）利用SDS-PAGE电泳研究了不同杀菌方式对牛乳总蛋白的影响，发现经UHT、巴氏和500MPa交变处理的牛乳与生鲜牛乳相比其总蛋白电泳图谱靠近负极方向第一条谱带消失，第二条谱带颜色变浅，由此推测经上述处理后牛乳中有一些蛋白质的亚基发生了解离，而经400MPa交变处理的牛乳与生鲜牛乳的总蛋白电泳图谱相似；4）超高压牛乳营养成分与生鲜牛乳相比，苏氨酸、VA、VC在超高压处理中受到的破坏明显比巴氏杀菌小得多，特别是苏氨酸和VA几乎完全被保留下来，而VC含量也要比巴氏奶高出25%以上，对于VB1、VB2，检测数据也表明，超高压灭菌对其破坏要比巴氏灭菌小，其含量分别比巴氏杀菌牛乳高出9.5%和2.6%；5）与巴氏杀菌乳相比，超高压杀菌有助于延长牛乳的保质期，扩大牛乳的销售半径。

Meringues ( Meringue Cookies , Meringue Hearts , Meringue Mushrooms , Pavlova ) are always a good way to use egg whites as is the Chocolate Angel Food Cake .

酥 （ 蛋白曲奇，心蛋白，蛋白蘑菇，帕夫洛娃）都是一个很好的方法，可使用的卵子是白人巧克力天使食品蛋糕。

The extracellular edelfosine promoted the phosphatization of Spmlvia inducing the expression of Mid2; The intracellular edelfosine increased the phosphatization of Spmlvia inhibiting the expression of Pmp1, and leading the holdback of cytokinesis.

细胞外依地福新诱导Mid2蛋白的表达，从而促进的Spm1磷酸化；细胞内依地福新通过抑制Pmp1蛋白的表达，取消Pmp1蛋白对Spm1的抑制作用，使Spm1的磷酸化程度增加，最终导致粟酒裂殖酵母细胞胞质分裂障碍。

Then, the experiment that edelfosine inhibited the phosphatization of Spm1 was carried out in order to elucidate whether edelfosine affect on the phosphatization of Spm1 induced via Mid2 and inhibited via Pmp1.(1)、On the inhibition experiment of parallel growth of S. pombe wild-type cells, spm1 mutants, pmp1 mutants, mid2 mutants and relevant retransform strains, the spm1 mutants grew well treated with 5.0μM and 10.0μM edelfosine for 24 h; the growth ratio of spm1 mutants had a statistic significance in between the cells previously mentioned and the S.

(1)、通过野生型粟酒裂殖酵母和胞质分裂突变体spm1Δ、pmp1Δ、mid2Δ及其再转化株对依地福新的平行生长抵抗试验，进一步确定依地福新对spm1、pmp1、mid2基因的影响；(2)、应用依地福新对Spm1磷酸化的影响试验，阐明依地福新是否通过影响Mid2蛋白和Pmp1蛋白的表达而影响Spm1蛋白的磷酸化。

The structure, photosensitization of phycocyanin and the principles of photodynamic therapy were mainly reviewed.

本文阐述了藻蓝蛋白的结构、光动力疗法的原理和藻蓝蛋白光敏作用的研究进展，并介绍了藻蓝蛋白在光动力疗法中的应用现状与前景。

The possible mechanism of phycocyanin in the proliferation inhibition of K562 cells is to increase the expression of FAK gene. The phcocyanin may be considered as a potential agent for inhibition of cancer cell proliferation.

藻蓝蛋白可能通过增强细胞基质与K562细胞表面的整合蛋白β1结合，上调K562细胞内FAK基因表达水平，恢复整合蛋白β1介导的细胞增殖抑制信号而发挥抑制K562细胞增殖的作用。

In order to explore the possibly involved morphological mechanism of R phycoerythrin mediated photodynamic killing at cell level, the fluorescence distribution of R phycoerythrin——a novel photosensitizer in tumor cells——was observed.

在荧光显微镜下观察新型光敏剂R藻红蛋白的荧光在肿瘤细胞中的分布，探讨以藻红蛋白为光敏药物的光动力损伤可能发生的细胞水平机制，以及培养液酸度和藻红蛋白浓度对其与细胞结合、进入及光动力杀伤的关系。

Simultaneously, according to different target proteins, these two mechanisms of PIAS proteins are not absolutely repulsive, which indicates the specificity and complexity of the function of PIAS proteins.

同时，针对不同的靶蛋白，PIAS蛋白的上述两种作用机制并不是完全互相排斥的，这体现了PIAS蛋白功能的特异性和复杂性。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。