蛋白

After induction and massive expression of the 3 fusion proteins, the soluble fraction was purified with 50% Ni-NTA resin affinity chromatography, which was proven by SDS-PAGE of the eluate to be a method that can achieve high purity of fusion proteins.

大量诱导表达三种融合蛋白后，取含FA、FB融合蛋白的可溶性组分用50％Ni-NTA树脂过柱纯化，将洗涤部分进行SDS-PAGE检测，表明用此纯化方法能纯化到高纯度的融合蛋白。

At day 4, embryoid body was found and cells were positive for actin, nestin and keratin.

培养第4天形成拟胚体，肌肉特异性肌动蛋白、巢蛋白、广谱细胞角蛋白均呈阳性表达。

Egg albumen not only has important nutrition value,and still have important functional property,for instance emulsibility ,foaming characteristic etc.

蛋清蛋白不仅具有重要的营养价值，而且还具有重要的功能特性如乳化性、起泡性等，作为一种重要的蛋白原料，本实验研究了高压脉冲电场对蛋清蛋白功能性质的影响。

Material and MethodsRA Rabbit Model group: 15 early RA rabbits of the same weight and variety Control group:10 normal rabbits of the same weight and varietyMethods of making RA rabbit model:elect 15 normal rabbits of the same weight and variety, dissolve Ovalbumin in 0.9% sodium chloride solution, to make the solution of 20mg/ml concengtration, blend the same quantity of complete Freund′s Adjuvant equably ,inject the mixed solution into endermic tissue of the rabbit′s scapular section, making the rabbits allergic, inject 1 ml of the mixed solution to one rabbit every time, inject 1 ml of the mixed solution in 5 different places of the rabbit′s scapular section, inject the rabbit one time every week,inject 3 weeks continuously,it turned out to be that the rabbits will be allergic, inject Ovalbumin blent with the 0.9% sodium chloride solution into the knee joint cavity of the rabbits in the fourth week, 5 mg Ovalbumin every knee joint cavity,two knees of every rabbit of the 15 rabbits are both injected,the arthrosis diameter and the exterior temperature of the knees will be added obviously in 24 hours,and they will drop gradually,at the time of the 14th or 21th day, the arthrosis diameter and the exterior temperature of the knees will get to the balance time,the incidence rate of RA is 100%.after the RA model succeeds,it is the early time of RA from the first week to the fourth week,after the fourth week,it is the late time of RA, the cartilage of the femoral condyle and the subchondral bone cortices will be changed unrecoverily.

资料与方法RA模型组：早期RA家兔模型15只，品种及体重接近。对照组：正常家兔10只，品种及体重接近。RA家兔模型制作方法：选取15只成年家兔，体重、品种接近，将卵蛋白溶解于生理盐水，配成浓度为20mg/ml的溶液，与等量完全弗氏佐剂混匀，注入家兔肩胛区皮下致敏，每只家兔每次注射1ml，于肩胛区5个不同区域注射，每周一次，连续注射3周而致敏，第4周向膝关节腔注射卵蛋白生理盐水溶液，每只关节腔注射5mg卵蛋白，15只家兔膝关节全部注射，24h内此关节直径和表面温度大幅度上升，以后缓慢下降，至14～21d达到平台期，发病率达100%。造模成功后第1～4周为早期改变，第4周以后出现不可逆的关节软骨及骨破坏。采用高频超声对RA模型组与对照组的膝关节髌上囊液体厚度、滑膜及股骨髁软骨厚度及软骨下骨皮质的回声情况进行对比观察。结果RA组模型组膝关节髌上囊积液及滑膜的厚度明显厚于对照组，其股骨髁软骨的厚度与对照组相比无明显差异，其软骨下骨皮质与对照组相比无明显改变。

The expressed IL-29 fusion protein was purified by Ni-NTA affinity chromatography and the fusion tag was removed from IL-29 fusion protein by cleavage with enterokinase.

纯化后的融合蛋白经肠激酶切割和回收后，所得目的蛋白（IL-29）纯度大于96％，该蛋白N-端序列与理论值一致，其抗病毒活性与IFN-α2b相当。

Lymphangiectasia is an intestinal disease of dogs characterized by chronic diarrhea and loss of proteins such as serum albumin and globulin. It is considered to be a chronic form of protein-losing enteropathy.

淋巴管扩张症是一种犬类的肠道疾病，其特点是慢性腹泻和蛋白流失，如血清白蛋白和球蛋白，被认为是一种慢性的蛋白流失性肠病。

Result:This example main clinical manifestation for the abdominal pain, the platoon is black then, epigastrium CT, the magnetic resonance and the gastroscopy prompt the duodenum tumor, the good duodenum tumor excision method, after the technique the pathology inspection shows the duodenum fusiform shape cell lump, the immunity group result shows tumor cell CD117, CD34, the union protein, the S-100 protein, the muscle moves the protein, finally diagnoses for the duodenum benignity the nature lump.

结果：该例主要临床表现为腹痛、排黑便，上腹部CT、磁共振和胃镜检查均提示十二指肠肿瘤，行十二指肠肿瘤切除术，术后病理检查示十二指肠梭形细胞瘤，免疫组化结果示肿瘤细胞CD117，CD34，结合蛋白，S-100蛋白，肌动蛋白，最后确诊为十二指肠良性间质瘤。

Comparing with database in GenBank, the results showed RBB1 had 91% homology to mRNA for a known ATP synthase CF1 epsilon chain 89% homology to ATPase epsilon subunit in Zea mays and 87% homology to Triticum aestivum ATP synthase CF1 epsilon chain.

对该基因可读框架编码的蛋白进行功能位点和结构功能域的分析，同样证明了该基因为H〓-ATP合酶CF〓亚基蛋白编码基因，其氨基酸序列3-83之间和1-133位氨基酸之间具有两个保守的蛋白结构域ATP-synt-DE-N和AtpC。

Methods: Rat models of chronic renal failure were made by cut 5/6 kindney or fed with feedingstuff which contains adenine(0.75%) for 4 weeks. Experimental treatment was applied by giving Yi Shen Granule and the curative effect of Yi Shen Granule on ongoing pathological changes of CRF was studied. According to the data of CRF rats in weight, water wastage, hemanalysis(RBC、HGB、HCT、MCV、MCH), urinalysis(volume, specific gravity, pH, proteinuria), blood serum analysis(BUN、Cre、ALB、TP), kidney weight coefficient and kidney morphology ( the grade of expansile renal tubule and the diameter of glomerulus ),the pharmacological effect of Yi Shen Granule could be ascertained.

实验选用0.75%腺嘌呤饲喂法致大鼠慢性肾衰模型和5/6肾切除法致大鼠肾衰模型，考察益肾颗粒对慢性肾功能衰竭进行性病变的影响，以造模动物一般状况观察、血液学指标包括红细胞计数、血红蛋白、红细胞压积、平均红细胞体积、平均红细胞血红蛋白(RBC、HGB、HCT、MCV、MCH)，尿液指标(尿量、比重SG、pH值、尿蛋白)、血清生化学指标包括尿素氮、肌酐、白蛋白、总蛋白(BUN、Cre、ALB、TP)、脏器解剖和病理观察指标(肾小管扩张分级评分和肾小球径长)做为判定依据。

They can be classificated to two kinds. One kind is build by photomultiplier; another kind is build by MCP. The mechanism of delayed luminescence is discussed with subradiation. A photon count system is built. To proof the biophoton is a kind of ultra weak photon stream originate from exothermic reaction, and primarily discuss the relationship between the delayed luminescence and proteins activity, we chose microphyllous banyan leave , ficus elastica leave, gingkgo leave, Chinese fan palm leave, and cinnamomum camphora leave as biological samples. The ultra weak photon emission from normal biosamples, and proteins inactivated biosample were measured separately with photon count system. The results showed that the delayed luminescence of biosamples have

在研究过程中，作者从辐射陷获的角度讨论了延迟发光的产生机制，对生物光子的生物化学起源与相干场起源的统一性进行了探索；自行搭建了单光子探测系统；测量了小叶榕树、印度橡树，银杏树，蒲葵树香和樟树等多种植物叶片样品的延迟发光，获得了大量实验数据；为了证明生物光子是一种起源于放热化学反应能量释放的微弱光子流，并初步探讨生物蛋白活性与延迟发光衰减之间的关系，测量了正常生物样品、生物蛋白灭活样品的延迟发光，同时测量了多种不含蛋白质的无机化学体系作为比对，实验结果和实验数据的回归分析表明：弱光子发射来源于放热的化学反应，延迟发光的强度与衰减时间与生物蛋白活性密切相关。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。