蛋白

Using the obtained antiserum as the first antibody, and the monoclonal antibody 4C11 as the collator, the specificity of the antiserum was tested by Western blotting and indirect-ELISA with the Yak recombinant mature PrP, GST fusion protein, the extract of brain and the bacterium protein.

以自制抗血清为一抗，单抗4C11为对照，对牦牛重组成熟朊蛋白、GST蛋白、脑组织提取物和菌体蛋白进行Western blotting检测和间接ELISA试验，验证自制抗血清的特异性。

It can be concluded that the extraction of SPI or CPI were significantly effected by different comminute granularity, the content of free gossypol were significantly decreased by base-dissolve and acid-deposit.

综上所述，不同的粉碎粒度对大豆分离蛋白和棉粕分离蛋白的提取率有显著的影响，碱溶酸沉法显著降低了棉粕分离蛋白中游离棉酚的含量。

Methods The levels of serum RBP and PA weremeasured by immunotransmission turbidity and serum ALB was detected by bromo2 creosol green method in 89 patients with hepatitis B cirrhosis and 35 healthy controls.

目的 联合检测视黄醇结合蛋白、前白蛋白和白蛋白在乙肝肝硬化患者中的血清水平变化，探讨三者的相关性及临床价值。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

Methods Of 219 early pregnancy women taking RU486,30 cases as effective group,23 cases as ineffective group and 16 cases as curette abortion group,and they were labeled immunohistochemically with PR,ER and EGFR,PKC,raf-1,MEK-1 and Bcl-2,respectively.

219例服用RU486早孕药物流产者，对其有效组30例，失败组23例，人流组对照16例，以免疫组化法分别标记孕激素受体、雌激素受体、表皮生长因子受体、酪氨酸蛋白激酶C、分裂激活蛋白激酶(MEK-1)、细胞转化分裂介质(raf-1)和抗凋亡蛋白(Bcl-2)。

Methods Of 219 early pregnancy women taking RU486,30 cases as effectiv e group,23 cases as ineffective group and 16 cases as curette abortion group,and t hey were labeled immunohistochemically with PR,ER and EGFR,PKC,raf-1,MEK-1 and Bcl-2,resp ectively.

219例服用RU486早孕药物流产者，对其有效组30例，失败组23例，人流组对照16 例，以免疫组化法分别标记孕激素受体、雌激素受体、表皮生长因子受体、酪氨酸蛋白激酶C、分裂激活蛋白激酶(MEK-1)、细胞转化分裂介质(raf-1)和抗凋亡蛋白(Bcl-2)。

Based on present application status of waste products in curriery in China, the advantages and disadvantages of collagen and analysis and characterization of collagen extracted from hideoffals, studies on collagen application properties and its blend films with one or two polysaccharides were done in this thesis.

本论文基于我国制革废弃物的应用研究现状及胶原蛋白的性能优势与缺点，在减轻制革污染的同时，变废为宝，以高值转化为目的，在对从制革皮边角余料中提取的胶原蛋白进行分析表征的基础上，围绕胶原蛋白的应用特性和共混生物膜材料进行了研究。

Collagen: Collagen can excite the fibre mother cell in the cutis layer of skin and let it reproduce the collagen. It can help renew cell, smooth skin and anti-age, tighten skin and reduce wrinkle accordingly.

胶原蛋白：胶原蛋白能刺激真皮层中的纤维母细胞，自行生成胶原蛋白，从而有效延缓肌肤的老化与松弛，去除皱纹，修复细胞，使肌肤细致平滑。

Besides these kinesins and chromosomal passengers, some centrosomal proteins also regulated cytokinesis.

除了马达蛋白和染色体旅客蛋白以外，一些中心体蛋白也参与了胞质分裂的调控。

Meanwhile we also assayed the variation of the Bt protein in the larval body and dejecta. By voidance of dejecta, the level of Bt protein in the larval body was always lower than that in dejecta alter feeding for 12 h, and the difference between both reached the highest at 24 h after feeding.

实验同时测定了幼虫体内及其粪便中Bt毒蛋白含量的变化，结果表明取食转基因水稻叶片12h后，随着大量取食进入体内的毒蛋白随粪便排出，幼虫体内的毒蛋白含量一直低于粪便中的含量，且在24h时两者差异达到最大。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。