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The method has thyroid transcribe factor to specimen of 12 SHL tissue - antigen of 1(TTF-1), epithelial film , CK7, CK20, dash forward the albumen that touch bubble , undee albumen , flowing flesh actin , placental alkalescent phosphoric acid is enzymatic , be addicted to bead albumen, among them observation of 10 lens that make report.

方法对12例SHL组织标本进行甲状腺转录因子-1(TTF-1)、上皮膜抗原、CK7、CK20、突触泡蛋白、波形蛋白、平滑肌肌动蛋白、胎盘碱性磷酸酶、嗜铬粒蛋白、CD34的SP免疫组织化学法标记,其中10例做电镜观察。

The correlation between Survivin and p53 expression and histological tyes and clinical stages in ovarian carcinoma were analysed.

结果: 1。卵巢癌p53和Survivin蛋白阳性表达率在卵巢癌组织中P53蛋白阳性表达率为54.84%(34/62)与正常对照组比较有显著差异(p<0.01);在浆液性囊腺癌中P53蛋白表达阳性表达率为56.52%(26/46)在黏液性囊腺癌中P53蛋白阳性表达率为57.14%(8/14)两组间无明显差异(p>0.05)。

Prediction the protein subnuclear locations; c we have for the first time extended the prediction scope to cover 22 subcellular locations, which greatly improves the practical value of the computational models.

本文首次在国际上提出并探讨了a 蛋白在细胞中多个位置出现的预测模型;b 蛋白在细胞核中出现的位置的预测模型,即&亚亚细胞位置预测模型&,获得国际学术界的认可;c 本文首次将亚细胞定位的预测研究推广到覆盖22个亚细胞位置,极大地提高了预测模型的实用价值,并提出了融合蛋白序列高层基因本体特征及序列自身氨基酸特征的蛋白亚细胞位置预测方法,提出了面向不同物种的亚细胞定位的预测新思路;结果表明新算法方法在严格的数据集上获得了比传统算法方法高出35%以上的预测精度,所开发的工具被广泛应用于生物实验中。

VCX/Y protein molecules are small size and high charge, which resemble those chromatin-associated proteins, such as histones or protamines, the latter mediate condensed DNA packaging in sperm.

依据氨基酸组成特点,该家族蛋白曾被推测为类似组蛋白和精蛋白的DNA或RNA结合蛋白

The 57 kD precursor, 37-39 kD acidic subunit and 22-23 kD basic subunit of glutelin and the 13 kD prolamine could be showed clearly.

各种水稻能够显示清晰的蛋白组分条带主要有:谷蛋白37~39 kD酸性亚基、22~23 kD碱性亚基、谷蛋白57 kD前体多肽和13 kD醇溶蛋白多肽。

And the mouse prions were able to change the hamster proteins into a new kind of prion that infected both healthy hamsters and mice.

老鼠朊蛋白能把仓鼠蛋白变为一种新的朊蛋白,这种新的朊蛋白能感染健康的仓鼠和老鼠。

These results suggested that the function of each function domain in HIV 1 LTR exhibited distinct characteristics on gag gene expression in vaccinia virus:(1) The function of intact LTR on gag gene expression varied with the upstream poxvirus promoter;(2) NR sequence neither decreased nor increased the Gag protein exprssion level;(3) Enhancer sequence might not be recognized by recombinant vaccinia virus expression system;(4) TAR sequence enhanced Gag protein expression effectively;(5) U5 region and its downstream non translated sequence had little effect on

免疫印迹和免疫酶试验检测均表明,6株重组病毒的Gag蛋白表达量因LTR不同而有明显差异,表明HIV1的LTR及其下游基因置于痘病毒启动子控制下,在痘苗病毒中表达时有下述特点:(1)不同的痘苗病毒启动子与全长LTR相互作用,对gag基因表达有显著不同的调控效果;(2)NR序列对Gag蛋白表达没有明显影响;(3)EN序列不能被重组痘苗病毒表达系统识别;(4)TAR序列可提高Gag蛋白的表达量;(5)U5区及下游非翻译序列不影响Gag蛋白的表达。

When stained with TRICT-conjugated phalloidin, both the nuclei and nuclear matrix specimens were found to give off specific, red fluorescent signals which represent the location of F-actin, and the pattern of TRITC signals distribution in the nuclei and nuclear matrix showed similarity with that of FITC signals.

在荧光显微镜下观察到:代表肌动蛋白的特意性荧光分布在细胞核和细胞骨架中,说明肌动蛋白是细胞核和核骨架的固有成分;代表F-肌动蛋白的特异性荧光存在于细胞核和核骨架中,说明细胞核和核骨架含有F-肌动蛋白

Northern blotting analysis indicated that 5 genes, nitrate transporter gene, nitrite reductase gene, ammonium transporter gene, ATP-binding cassette transporter gene, and purine permease gene, were significantly up-regulated under nitrogen starvation.

Northern blotting验证其中5个基因,包括硝酸盐转运蛋白基因、铁氧化还原蛋白亚硝酸还原酶基因、铵盐转运蛋白基因、结合ATP盒的转运蛋白基因和嘌呤透过酶基因,在缺氮诱导条件下转录水平有明显上调。

Conclusions-This is the first study to demonstrate that intestinal TRL apoB-48 production is increased after short-term elevation of plasma FFAs in humans in the fed state, similar to the well-described stimulation of hepatic TRL apoB100-containing particles by FFAs.

结论-这项研究首次表明人类在进食状态下血浆游离脂肪酸短期升高会促进肠内载脂蛋白B-48甘油三酯脂蛋白的合成,与多项研究证实的游离脂肪酸可促进肝脏载脂蛋白B-100甘油三酯脂蛋白的合成相似。

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