蛋白

Methods: The hospitalized cases with EH and HS were collected in a 3-A grade hospital of Xi'an city (from Jun. 2001 to Mar. 2005) according to the includible standard and excludible standard. Unified questionnaires were filled in. The items of questionnaires included: sex, age, inhospital days, death in hospital, first-measured blood pressure Systolic blood pressure (SBP, Diastolic blood pressure, Pulse pressure, blood lipid total cholesterol (TC, Triglyceride, High density lipoprotein cholesterol, Low density lipoprotein cholesterol, Apolipoprotein A and B.

检索西安市某三级甲等医院2001年6月－2005年3月期间EH合并HS住院病例，按照纳入标准和排除标准筛选病例资料，填写统一调查表，调查项目包括：性别、年龄、住院天数、死亡情况、首测血压、血脂（总胆固醇、甘油三酯、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、载脂蛋白A以及载脂蛋白B）等指标，并按性别与年龄分层进行回顾性分析和研究。

In addition to the two axial ligations provided by His18 and Met80, the hydrogen bond and hydrophobic interactions between the heme and the protein polypeptide, the heme also covalently linked to the protein matrix through two thioether bonds which are formed between vinyl groups of the heme and cysteine residues of a classic Cys-Xaa-Xaa-Cys-His heme-binding motif of the polypeptide chain.

Cyt c是血红素蛋白中电子传递蛋白的典型代表，其辅基heme与蛋白肽链的结合除了来自heme轴向配体组氨酸(His18)和甲硫氨酸(Met80)的配位作用、氢键和疏水相互作用等非共价键作用方式外，血红辅基heme的两个乙烯基还与蛋白肽链上的结构片段域Cys-Xaa-Xaa-Cys-His中的两个半胱氨酸形成硫醚键以共价键方式相结合。

The results showed that under the cold stimulation, the types of blood protein both changeed, cold shock proteins was generated. Since the different genes which control CSPs need different intensity and degree of stimulation, the same specie of Loaches had the differences in the time and new protein generation under the different temperature treatment. Since two types of Loaches have different adaptability, the time and type of CSPs generation are also different under the same temperature treatment.

结果表明，泥鳅和大鳞副泥鳅的血液蛋白质发生了变化，即产生了冷激蛋白；同种泥鳅，控制产生冷激蛋白的特定基因在一定的刺激强度和刺激程度下，产生冷激蛋白的时间和种类不同；不同种泥鳅，在相同低温处理条件下，产生冷激蛋白的时间和种类也不一样。

Eur J Haematol, 1996, 57:121～127.［3］ Enzan H, Takahashi H, Kawasaki M et al.Light and electron microscopic observation of hepatic hematopoiesis of human fetuses.

BFU-E在增殖分化过程中高表达周期蛋白E，并轻度上调周期蛋白D3，分化末期周期蛋白E和周期蛋白D3的表达迅速下调［14］。

At concentration of 2% PEG8000, 30% microsomal protein containing 10% of total P450 was precipitated. At concentration of 5% PEG8000, 55% microsomal protein containing 20% of total P450 was precipitated. At concentration of 8% PEG8000, 78% microsomal protein containing 30% of total P450 was precipitated. At concentration of 11% PEG8000, 85% microsomal protein containing 70% of total P450 was precipitated.

当PEG的浓度为2％时，约30％的微粒体蛋白和10％的P450被沉淀；PEG为5％时，约55％的微粒体蛋白和20％的P450被沉淀；PEG为8％时，约78％的微粒体蛋白和30％的P450被沉淀；PEG为11％时，约85％的微粒体蛋白和70％的P450被沉淀。

In prophase, it localized on the microtubule organizing-center. In metaphase, it co-localized with α-tublin completely on spindle. In anaphase, it co-localized with α-tublin in aster and mid-zone. In telophase, it co-localized with α-tublin in cytoplasmic bridge connecting the two daughter cells.

在分裂前期，hSMP-1蛋白位于微管组织中心区域；在分裂中期，hSMP-1蛋白与组成纺锤体的微管呈完全共定位；在分裂后期，hSMP-1蛋白与星体和纺锤体中区的微管呈共定位关系；在分裂末期，hSMP-1蛋白则与两个子细胞之间的胞质桥完全共定位。

Single mucin or either two mucins were sensitive and specific in the diagnosis of gastric cancer. For single mucin detection, MUC1 was the most sensitive and specific, with a sensibility of 81.5% and a specificity of 75.8%, respectively. Mucins in combination (at least 2 positive) had a sensibility of 96.0% and a specificity of 82.9%, respectively.

各单一黏蛋白以及黏蛋白两两之间联合用于胃癌诊断的敏感性及特异性均较高，其中单一黏蛋白中， MUC1的的敏感性和特异性最好，分别达到81.5%、75.8%，联合诊断(3种黏蛋白中至少2种阳性)的敏感性和特异性达到96.0%、82.9%。

The monoclonal antibody was produced by the method of inoculating BALB/c mice intraperitoneally with the hybridoma cells, and purified with protein A-Sepharose 4B affinity chromatography.3 Establishing of ELISA protocol to detect hBLySTwo kind of different ELISA protocols were tried to study the experiment condition of detecting hBLyS level. Streptavidin was used to indirect coating in protocal 1, but biotin-avidin system was used to enlarge the final reaction signal in protocal 2. The optimal reaction condition was chosen by the experiments. The sensitivity of protocal 1 was poor, but the linear relation of protocal 2 standard curve was excellent when the concentration of hBLyS was between 3.2~400 ng/mL. So a protocal was established which sensitivity can reach to nanogram.

建立检测hBLyS蛋白含量的ELISA方法选用2种ELISA方案对hBLyS蛋白含量的测定方法进行研究，方案一用链霉亲和素做间接包被，方案二用生物素-亲和素系统做终反应放大；优化选择2种检测方法的反应条件；方法一的敏感度较低：方法二在hBLyS蛋白浓度为3.2～400 ng/mL范围内，标准曲线的线性关系良好；从而建立了一种可检测hBLyS蛋白浓度为ng级水平的ELISA方法。

VP6 is encoded by genome segment 6 and is the major structural protein in virus particles, itconstitutes approximately 50% of the virion by weight.

VP6蛋白是轮状病毒第六个基因编码的内壳蛋白，而且也是主要的结构蛋白，约占病毒蛋白的50％。

It is suggested that six OMPs ofV.parahaemolyticus ompV, ompW, ompK, ompU, psuA and pvuA, are immunogenicduring in vivo infection, and may be of great significance for vaccine development.Especially, fish group immunized with recombinant ompK achieved a high protection of90%. For the wide expression of this conserved protein among vibrio species, ompKmust be paid enough attention for vaccine development against vibriosis.

研究结果显示了6种副溶血弧菌外膜蛋白ompW、ompV、ompK、ompU、psuA和pvuA具有良好的免疫原性，是宿主免疫应答的保护性抗原；尤其是ompK单个蛋白免疫组获得90％的保护率，由于该蛋白是弧菌中广泛存在的保守性蛋白，极有可能作为制备亚单位疫苗等高效弧菌疫苗的候选抗原。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。