蛋白

After binding with ligands, the RTKs would dimerize, simultaneously autophosphorylated and activated and then trigger the downstream signaling pathway.

大量工作表明，配体与受体结合，引起受体的二聚化并同时激活受体后，受体可结合大量的信号蛋白分子，如接头蛋白、入坞蛋白或各种调节蛋白等。

I. Design and selection of thc high affinity DNA ligands for singIe-chain reprcssorSingle-chain repressor RRn., is a derivative of bacteriophage 434 repressor,which contains covalent1y dimerized DNA-binding domains (axnino acids 1-69) of thephage 434 repressor.

一。设计和筛选单链阻遏蛋白的高亲和力DNA结合序列单链阻遏蛋白RR_是噬菌体434阻遏蛋白的衍生物，它是噬菌体434阻遏蛋白的N端DBD（1—69位氨基酸）组成的共价二聚体。

Molecular dynamics calculations were performed to monomers and dimers of three kinds of metallothioneins in water solution.

对三类金属硫蛋白（大鼠金属硫蛋白亚型II，兔肝金属硫蛋白亚型I和兔肝金属硫蛋白亚型II）的单体和二聚体进行了水溶液条件下的分子动力学模拟。

Dorsalis by salting out, ion exchange chromatography, and gel filtration chromatography. By salting out the egg homogenate, SDS-PAGE analysis revealed that the Vns were mainly precipitated in a 50~70% ammonium sulfate solution. The salted-out proteins were further purified by ion exchange chromatography, and the result showed that Vns were mainly eluted with a 0.3~0.5 M sodium chloride gradient. Finally, the elutes of ion exchange chromatography were refined by running them through gel filtration chromatography to obtain purified Vns. SDS-PAGE separated the 48- and 51-kDa purified Vns, and used them as antigens to raise polyclonal antibodies.

利用硫酸铵盐析法初步分离果实蝇卵中的蛋白质，以SDS-PAGE蛋白质电泳分析得知，卵黄蛋白主要在硫酸铵溶液浓度为50~70%时被沉淀出；盐析沉淀所得之卵黄蛋白，再以离子交换层析法进一步纯化显示，卵黄蛋白主要会在0.3~0.5 M氯化钠的浓度范围间被析出来；再将析出液浓缩后，经凝胶过滤层析法做最后的纯化，即获得纯化之卵黄蛋白。

There was no difference between breast cancer and fibro adenoma tissues in ERαexpression.The expression of ERαin breast cancer tissues was not associated with histological types,age,clinical stage,lymph node metastases state,HER-2 and P53 protein expression.

结论1、乳腺癌组织ERα蛋白表达与乳腺纤维腺瘤组织比较无显著差异；乳腺癌组织中ERα蛋白表达与患者年龄、病理类型、临床分期、淋巴结转移、P53、HER-2蛋白无关，与PR蛋白表达正相关。

MyosinⅩ, a vertebrate-specific member of myosin super-family, can be detected in most tissues and cells, such as kidney, liver, brain, and so on. Full-length MyosinⅩcan stimulate the formation or stabilization of filopodia. As an actin-based motor protein, MyosinⅩplays a key role connecting cell membrane proteins and cytoskeleton.

肌球蛋白Ⅹ是脊椎动物所特有的细胞骨架蛋白，在脑、肝、肾等大多数组织中都有表达，它的显著特点是定位于细胞丝足的顶端和片状伪足的边缘，MyosinⅩ作为一个动力蛋白分子，起到连接细胞膜蛋白与细胞内骨架蛋白的桥梁作用，因此推测它可能在SVZa成神经细胞的迁移过程中起到重要作用。

After the sequence and open reading frame were confirmed by DNA sequencing, the GADes gene was expressed in E.coli. SDS-PAGE analysis suggested that the bacteria BL21 produce a kind of fusion protein of 92KD and the expressed GADes fusion protein shows expected immunoreactivity by Western Blot.

SDS-PAGE电泳和Western Blot分别鉴定融合蛋白大小和免疫活性后，对表达条件进行优化，对包涵体进行分离、溶解和复性处理，以提高可溶性融合蛋白的产量，使融合蛋白达总蛋白的50％，相当于1.495g/每升培养物。

For all of GAS toxicity factors, M protein is the most important toxicity factor. M protein is a membrane protein which have α-helical coiled coil structure on the plasma membrane. It helps GAS to attack the host and have resistance to the immune system.

在A型链球菌所有的毒性因子中，M蛋白是最重要的毒性因子。M蛋白是A型链球菌上的一种膜蛋白，在A型链球菌细胞膜上形成α-helical coiled coil结构。M蛋白帮助A型链球菌侵入宿主并且逃避宿主的免疫系统攻击。

Through optimization of thick mash fermentation conditions, suitable fermentation conditions for improving solubility of corn protein were determined, so as to improve the solubility of corn protein favorable for digestion of protein by animals, so that the utilization rate of the corn gluten meal as a feed could be increased.

通过甘浓醪发酵条件的优化研究，确定适宜的改善玉米蛋白溶解性的发酵条件，提高玉米蛋白的溶解性，有利于动物对蛋白的消化吸收，从而提高玉米蛋白作为饲料的利用率。

Protein phosphatases are crucial for cell living. Protein phosphatase 2A (PP2A) is an important member of serine/theronine protein phosphatase family, which is involved in almost all biological processes in eukaryotic cell. The 3D structure of PP2A core enzyme and holoenzyme was solved in 2006. These results are significant instructions for us to further understand PP2A structure, interactions between PP2A subunits, and also interactions between PP2A and its binding proteins.

蛋白磷酸酶在细胞的生命活动中起着十分重要的作用，蛋白磷酸酶2A(protein phosphatase 2A， PP2A)作为蛋白磷酸酶家族中十分重要的一员，它几乎与所有真核细胞的生命活动都有密不可分的关系。2006年，PP2A核心酶和全酶晶体结构的陆续破解对于深入了解PP2A自身的结构和亚基之间的相互作用，以及其与结合蛋白作用的机制都有重大的影响。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。