蛋白

The tick ovary development and proteins were affected by JHⅢ, 0.50 ng/μl and 1.00 ng/μl dosages could significantly promote the total protein concentration, 0.50 ng/μl JH Ⅲ increased vitellin quantity and ovary absorption for vitellogenin.

JHⅢ对卵巢发育和卵巢蛋白有影响，0.5ng/μl和1.0ng/μl显著提高卵巢总蛋白水平，0.50 ng/μl JH Ⅲ使卵黄蛋白合成量增加、促进卵巢对卵黄原蛋白的摄取。

The reacting tite with NDPK-A was 1:800 and theantisera was specific.Conclusions: The purified rhNDPK-A protein exists mainly in the form of hexamer;rhNDPK-A protein can promote the survival of spleen cell and may take part in theadjustment of cell growth by the way of changing cell cycle.

ELISA法检测所制备的抗体的反应效价为：1：800，western blotting试验结果证实，该抗血清具备较好的特异性，能与rhNDPK-A蛋白发生反应，结论：rhNDPK-A蛋白可能主要以六聚体形式存在，直接观察的蛋白结构与文献报道的X衍射结果相似；rhNDPK-A蛋白对正常小鼠的脾细胞的存活有促进作用，推测很可能以信号转导的方式调控细胞周期从而达到对细胞生长的调节作用。

However, higher concentration of CRP may increase the expression of protein and mRNA of inflammatory factor in endothelial cells; 2 Higher concentration of CRP may participate in the development of inflammatory process in endothelium by the mechanism that CRP induce cells apoptosis and activate signal pathway involved in cell proliferation. 3 CRP is the member of pentraxins family, in which two faces of pentamer form a special bi-phase structure: one face is ligand recognition phase; the other face is effector phase.

总结： 1CRP是一种保守蛋白，在正常生理水平下对内皮细胞没有明显的诱导炎症发生的作用，仅在高浓度条件下有可能诱导细胞炎症相关蛋白及mRNA的表达； 2高于生理水平的CRP对内皮细胞的致炎作用主要通过诱导细胞凋亡及激活细胞增殖相关蛋白信号通路的表达； 3CRP是一种双面五聚体蛋白，结构具有特殊的二相性，能够分别结合内皮细胞和E-LDL。

These proteins can be used to preserve cells, tissues and organs from desiccating at low temperatures, thus AFPs could be used in the organ transplantation.

昆虫抗冻蛋白在空间结构方面不同于植物抗冻蛋白和鱼类抗冻蛋白，其热滞活性远远高于这两种蛋白。

Complete genome sequences of 20 viruses in Bunyaviridae loaded from NCBI were analyzed by two biological softwares of DNAMAN and DNASTAR. The results indicated great variability in both nucleic acid sequence and protein structure between plant viruses and animal viruses:(1) only plant viruses could encored NSm in M genome;(2) the length of nucleic acid sequence and protein sequence was different;(3) GC content of nucleic acid of animal viruses was higher than plant viruses of that;(4) protein topology analysis by online software SMART discovered that there was significant difference for the structure of glycoproteins G(subscript nG between animal-infecting and plant-infecting viruses;(5) usually, protein of plant-infecting virus had more complicated structure, more low compositional complexity and had N-Signal peptides except INSV.

本文从NCBI数据库下载具有完整基因组序列的布尼亚科病毒的5个属20种病毒的序列，用生物学软件DNAman，DNAstar进行比对分析，发现布尼亚科病毒属中的植物病毒在核酸序列及蛋白结构上与动物病毒有很大差异：(1)只有植物病毒的M基因组能编码NSm运动蛋白；(2)植物病毒和动物病毒在核酸序列和蛋白质序列长度均有差异，表明该科病毒是进化速度较快的病毒；(3)植物病毒核酸序列的GC含量低于动物病毒；(4)通过SMART网络软件进行蛋白质拓扑结构分析发现植物病毒和动物病毒在糖蛋白GG的结构上存在显著差异；(5)植物病毒糖蛋白结构较为复杂，有较多的紊乱区域，除INSV外，其他病毒都具有N端信号肽。

Methods and materials A549 cells were collected and lysed for total protein after treated with metformin (5 mM) for 0, 15, 30 and 60 minutes. Western blot analysis was performed to detect the expressions of pan- and pho- AMPK, JNK and p38 proteins. Cleaved Caspase-3, 8, 9 proteins were also detected.

以5mmol/L的二甲双胍对A549细胞进行体外干预，在干预的第0、15、30和60min时，分别抽提细胞总蛋白，以Western blot技术检测细胞内AMPK、JNK、p38 MAPK的总蛋白和磷酸化蛋白的表达；在干预后第0、12、24和48h，检测剪切后的Caspase-3、8、9活性蛋白的表达水平。

The shorter variant mHemk2 is 1696 bp in length encoding a putative mouse protein of 14.7 kDa, consisting of 138 amino acid residues.In protein data bank annotations, Hemk has previously been classed among methyltransferases methylating N6-adenine or N4-cytidine in DNA because of the presence of S-adenosyl-L-methionine binding motif and of an NPPY motif in the protein.

在蛋白数据库的注释中，由于在Hemk蛋白中存在S-腺苷-L-甲硫氨酸结合基序和一个NPPY基序，因此，在蛋白库的说明中已经将Hemk蛋白归类于能甲基化DNA中N6-腺嘌呤或N4-胞嘧啶的甲基转移酶。

Moreover, the nitrated tau showed a significantly decreased binding activity to taxol-stabilized microtubules in vitro.

为阐明tau蛋白的硝基化在AD的病理过程中如何发挥作用，我们在体外用PN处理纯化的人重组tau蛋白，结果显示PN浓度依赖性地诱导tau蛋白发生硝基化和寡聚化，而且硝基化tau蛋白与微管的结合活性显著下降。

Enzyme activity assay demonstrated that the Trx-BTGase expressed in E. coli could be functional in polymerizing other proteins, and no influence on the biological activities of BTGase to polymerize BSA was found in case of the thrombin cleavage of this fusion protein.

酶活性分析表明表达的Trx-BTGase融合蛋白具有交联蛋白的活性，并发现Trx-BTGase融合蛋白和经凝血酶酶切后得到的BTGase单体都能催化牛血清白蛋白的聚合反应。

We also strongly suggest that the recently reported chaperone acitivity of periplasmic PDI-ase and PPIase assayed by traditional substrate should be reexamined using periplasmic proteins as substrate due to the significant difference between those two classes of proteins, the possible chaperone fuctions of PDIase and PPIase in vivo should be reappraised.

同时，由于本实验结果显示膜间质蛋白与常用的分子伴侣测活底物蛋白在聚集行为上存在极显著的区别，我们建议对最近报导的膜间质蛋白二硫键异构酶和脯氨酸顺反异构酶分子伴侣活性以膜间质蛋白为底物再进行验证，对其在膜间质是否发挥分子伴侣功能重新慎重考虑。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。