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METHODS: Seralbumin, serum creatine, blood sugar, total cholesterol, total triacylglycerol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol were detected after more than 12 hours of fasting.

测定过夜空腹12 h以上血白蛋白、血肌酐、血糖、总胆固醇、总三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇;用放射免疫法测定早晨第1次尿中白蛋白

Objective To explore the concentration changes of the serum acute-phase proteins,including α1-acid glycoprotein,α1-antitrypsin,haptoglobinand cernloplamin in patients with rheumatoid arthritis and seronegative spondyloarthropathy in active stage and their clinical significance.

目的 观察活动期类风湿性关节炎和血清阴性脊柱关节病患者血清4种急性时相反应蛋白α1-酸性糖蛋白、α1-抗胰蛋白酶、触珠蛋白和铜蓝蛋白浓度的变化及其临床意义。

The composition and structure of Soybean Protein Isolate are first introduced,Then the functional modification of SPI via chemical ways which include the reaction of amino group,carboxyl group and sulphydryl group,via enzymic ways which include the modification using transglutaminase enzyme and papain,via physical ways which include blending and heat treatment are reviewed.

首先介绍了大豆分离蛋白的基本组成与结构,然后分别从化学改性、酶改性和物理改性三个方面对大豆分离蛋白改性进行了综述。其中,在化学改性方面,针对大豆分离蛋白中含有的氨基、羧基、巯基等不同活性基团的改性原理及研究现状进行了介绍。在酶改性方面,主要介绍了谷胺酰胺转胺酶、木瓜蛋白酶等对大豆分离蛋白的改性作用。在物理改性方面,介绍了共混、加热改性等目前研究较多的方法。

Beta-thalassemia major is a serious hereditary hemolytic anemia which does great harm to human being.It is a monogenic disease characterised by a reduced synthesis of beta-globin chains,which dues to the Doint mutation or deletion of beta-globin gene and its control region.It not only decreases the hemoglobin level,but breaks the balance of alpha/beta synthesis ratio.The major cellular pathogenetic mechanisms in beta thalassemia are based primarily on the deleterious effects produced by the accumulation of the excess alpha globin chain.These excess alpha-globin chains cannot form stable tetrameric structure and deposit in erythroid cells.They cause large ineffective erythropoiesis on their own,accelerate red cells destruction,and lead to hemolysis.

地中海贫血是一种对人类健康危害严重的遗传性溶血性贫血病,是因β-珠蛋白基因及其调控序列的点突变或缺失致使β-珠蛋白肽链合成减少或完全停止,这不仅使患者血红蛋白水平降低,而且使原来在数量上与之持平的α-珠蛋白肽链相对过剩,这些相对过剩的游离α-珠蛋白肽链并不能形成稳定的四聚体,它们沉积在红细胞中导致了大量无效红细胞生成和红细胞寿命缩短,引起了严重的溶血性贫血。

SGK is a novel member of serine/threonine protein kinase family that is transcriptionally regulated. As an adaptor or scaffold protein, 14-3-3 protein plays important role in a variety of signal transduction pathways.

SGK是丝氨酸/苏氨酸蛋白激酶家族的一个新成员。14-3-3蛋白作为榫头蛋白或支架蛋白,在许多信号传导途径中起重要作用。

One of them was identified as aldehyde dehydrogenase 2, and the others were both identified as mouse RIKEN cDNA 2900053E13, suggesting that the protein might be post translationally modified.

通过对差异蛋白的胶内酶解和高效液相色谱/离子阱质谱鉴定,发现其中一个蛋白点为乙醛脱氢酶2(aldehyde dehydrogenase 2),另外两个差异点鉴定结果为同一个蛋白RIKEN cDNA 2900053E13,提示该蛋白在正常和心肌缺血心肌的线粒体内修饰状态发生了变化。

The results indicated that immobilized E2 protein remarkably raises the level of phosphorate-tyrosine in Raji cells , but the soluble E2 protein hasn't obvious impact to phosphorylation of tyrosine of Raji cell. It was presumed that immobilized E2 protein was resembled to natural viron enveloped E2 protein and may more efficiently interact with CD81 molecule.

这可能是因为固相化的E2蛋白类似天然的病毒颗粒,在构象上要更接近于天然的病毒颗粒表面E2蛋白,而且固相化后的E2蛋白在单位体积的密度要高于可溶性的E2蛋白单位体积的密度,使其能够更充分的与细胞表面的CD81分子相互接触作用。

At present, the research mainly concentrates on vitellin purification and characterization, vitellogenin synthesis and hormonal regulation, vitellogenin absorption and processing.

目前,对蜱类卵黄发生的研究主要包括卵黄蛋白的纯化与鉴定,卵黄原蛋白的合成与调控,卵母细胞对卵黄原蛋白的摄取以及转变为卵黄蛋白的分子过程。

Using indirect ELISA, the contents of vitellin in the fat body, haemolymph and ovary of P. japonica were detected. The results suggested that the vitellogenin was synthesized in fat body at the 2nd day after eclosion. The contents of vitellogenin in fat body, haemolymph and ovary increased quickly on the 4th day after eclosion and reached the peak stage approximately on the 8th day in adult stage.

采用间接竞争ELISA法,系统测定了龟纹瓢虫成虫期脂肪体、血淋巴和卵巢中卵黄蛋白的动态变化,结果表明:脂肪体是卵黄原蛋白合成的场所,卵黄原蛋白的合成始于羽化后第2天;脂肪体、血淋巴和卵巢中卵黄原蛋白的滴度在羽化后第4天开始迅速上升,至成虫期的第8天左右达到高峰期。

Yolk protens of Bombyx mandarina are also composed of vitellin, egg specific proteins and 30K proteins on the base of SDS-PAGE of egg subsracts of Bombyx mori and Bombyx mandarina.

通过家蚕与野桑蚕卵的内容物的SDS-PAGE,分析了野桑蚕卵黄蛋白主要有卵黄磷蛋白,卵雌异性蛋白和30K蛋白组成。

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