蛋白

Now the mechanism of action of heat shock protein is not quite sure to us.Understanding the biological function and its transplantation immunity of HSP will be helpful in investigating the relationship of HSP and graft ischemical reperfusion injury,graft rejection,HSP gene transfer,cardiac graft vascular disease.

目前，还不能确定热休克蛋白在上述反应过程中的直接作用的机制，了解热休克蛋白的生物学功能及其在移植免疫中的作用，有助于进行热休克蛋白与缺血/再灌注、移植排斥反应，以及热休克蛋白转基因治疗移植物血管病关系的研究。

The rice bran protein can be separated by GP-HPLC with AUC buffer solution. 40 gliadin components and 20 low-molecular weight glutenin subunit could be separated by HPCE with the buffer solution of alkaline sodium borate from wheat. Therefore the differences between varieties can be identified.

用AUC缓冲液和排阻色谱能有效分离米糠蛋白；用毛细管电泳和碱性硼酸钠缓冲液分离小麦蛋白，可得到40多个醇溶蛋白组分和20多个低分子量谷蛋白亚基，以此可鉴别品种差异。

Patatin is the trivial name for a family of glycoproteins that accounts for up to 40% of the soluble protein in potato Solanum tu...

在马铃薯块茎可溶性蛋白中，有一组分子量约为40kD的糖蛋白，占块茎总可溶性蛋白的40％左右，该组糖蛋白称为patatin。

In this article, we used automotive immunohistochemical stainer to check the expressions of HBsAg and HCV antigens in 100 cases of HCC and their adjacent tissues, mean while, we checked their histological activity index and hepatic fibrosis degree. We also used radioimmunoassay to detect serum levels of 4 serum fibrosis markers and serum AFP levels in these cases. Immunofluorescence labeling and flow cytometry were used to check the T-cell subpopulations and the activity of NK cells of these 100 cases. Then the correlations among these factors were studied.

本文采用全自动免疫组化仪对100例肝癌组织及癌旁组织中的HBsAg、HCV抗原表达进行了标记，同时对其进行肝组织活动指数、纤维化分期；同时采用放射免疫测定方法检测同期血清透明质酸、Ⅲ型前胶原蛋白、层粘连蛋白和Ⅳ型胶原蛋白等四项血清肝纤维化标志物和血清甲胎蛋白水平；采用免疫荧光标记技术和流式细胞仪对T细胞亚群及NK细胞活性测定，对各项指标的相关性进行系列对比研究。

According to the fluorescence measurement results from mixed solution, the association constant estimated for thioridazine hydrochloride-BSA binding and the number of the binding site with Scatchard analysis were 1.2×104 L/mol and 0.98, respectively.

将盐酸硫利达嗪与牛血清白蛋白以不同比例混合并在37 ℃下培养，以流动注射透析采样技术从混合液中分离出游离盐酸硫利达嗪，在线氧化为强荧光化合物后测定其浓度；应用Scrathard方程分析所测数据，求得盐酸硫利达嗪和牛血清白蛋白的结合常数为1.2×104 L/mol，结合位点数为0.98；根据热力学参数推断，盐酸硫利达嗪与牛血清白蛋白之间以疏水作用力为主；进一步基于荧光猝灭现象和Fster非辐射能量转移理论，得到盐酸硫利达嗪小分子与牛血清白蛋白之间的能量转移效率( E =0.497)和结合距离( r 0=3.27 nm)。

In this paper, we studied M. meretrix ferritin, cathepsin B and caspase genes, which are involved in clam larval shell formation, nutrition, metabolism and apoptosis, respectively. We have cloned the three genes, investigated the temporal and spatial expression profile both at gene and protein level in trochophore (L1), D-veliger (L2), pediveliger (L3) and postlarvae (L4). The potential roles of these proteins were analyzed with specific inhibitors during larval development. Firstly, embryos were found developed into trochophore-like larvae with no shell if cultured at gastrula stage in artificial seawater without iron. Shell-like structures were formed only in the presence of iron. The larvae which had been transferred at L1 stage into ASW developed normal shell. This indicated that iron and iron associated protein are important for larval shell formation. The EST sequence which is homologous with ferritin, which is a principal iron metabolic protein, was selected from the M. meretrix cDNA library. The full-length of ferritin subunit cDNA was cloned by RACE. The results of real-time PCR revealed that the MmeFer mRNA expression changed before and after the larval shell formation.

本论文以文蛤幼虫为研究对象，分别对文蛤幼虫发育过程中贝壳形成相关的铁蛋白、营养及变态相关的组织蛋白酶B及变态过程中细胞凋亡相关的caspase三个基因进行了克隆，分析了基因及编码蛋白在担轮幼虫期(L1)、D形幼虫期(L2)、壳顶幼虫期(L3)和稚贝期(L4)的时空表达特征，解析了其可能的功能，并研究了相应酶类的特异性抑制剂作用对幼虫发育过程的影响，进行了目标蛋白的功能验证，详述如下：研究结果显示，在文蛤胚胎发育到原肠胚时放入不含铁离子的人工海水中培养，发育成无壳的畸形，随着人工海水中铁离子添加浓度的升高，幼虫长出壳状组织接近正常状态；而发育到L1期幼虫放入不含铁离子的人工海水中培养却可以发育出正常的壳，推测铁和铁代谢相关蛋白在幼虫贝壳初始形成有重要的作用。

Some approved aneugens Trip terygiuwlIypogaucum (evelHutch, colchicine, thiabendazole, a tropine sulfate,d-ubocurarine was added into the reactive system respectively and the changes of their absorption values were taken down during different periods under the conditions of 37 0C and 350nm .

本研究通过多步聚合和解聚合反应从猪脑中分离纯化微管蛋白，建立微管蛋白的体外聚合和解聚合反应平衡体系，并将已证实的非整倍体诱发剂（昆明山海棠根部水抽提物、2-（4′-噻唑）苯丙咪唑、硫酸阿托品、d-筒箭毒碱）分别加入此反应平衡体系中，于37℃、350nm条件下，记录此体系不同时期的吸光值变化，与阳性对照物和溶剂对照处理平衡体系后的吸光值在相同时间点作比较，以此评估这几类非整倍体诱发剂对离体条件下的微管蛋白聚合的影响，寻求这几类非整倍体诱发剂是否可通过抑制微管蛋白聚合作用途径而诱发非整倍体。

Quantitative analysis of protein adsorption: The PS suspension containing lysozyme was subjected to ultracentrifuge (15 000 r/min) to collect clear aqueous solution. The lysozyme concentration in the clear solution was measured by spectrophotometry at 280 nm. The amount of protein adsorbed on PS surface was calculated based on the decrease in the protein concentration in the supernatant solution.

蛋白吸附定量分析：采用高速离心(15 000 r/min)法从含溶菌酶蛋白的聚苯乙烯悬浮液中分离出上清液，用紫外(280 nm)分光光度法测定经聚苯乙烯微球表面吸附后，液相蛋白质量浓度的下降值，由此推算蛋白在聚苯乙烯表面的吸附量。

The results showed that about610 protein spots could be visualized on the two dimensional electrophoresis map bycoomassie brilliant blue staining within Mr 9.0～100.0kD and pH 4～7,and about 94 spotswere differential expressed between male-sterile line and its maintainer line by PDQuestsoftware.Among those differential proteins,about 49 spots and 40 spots were differentialexpressed between uninucleate anther stage and binucleate stage respectively;Meanwhilecomparing with the 2-DE maps of the total proteins from the binucleate anther stage anduninucleate stage,15 differential spots were detectable at male-sterile line;and 16 differentialspots were detectable at its maintainer line.

在分子量9.0～100.0 kD、等电点4～7线性范围内，可识别约610个蛋白质点，PDQuest软件分析结果表明：在单核期不育系和保持系之间存在40个差异蛋白质点，二核期两者共有49个差异点；不育系在单核期和二核期两者之间存在有15个差异点，保持系在两个不同时期有16个差异点；不育系与保持系间在表达量上的差异蛋白质点以及不育系与保持系间特异表达或特异缺失的蛋白质点很可能与小麦雄性不育有关。3。

objective to study gastrodia elata blume to effect tau protein,sod,mda expression in gyrus hippocampi and cerebral cortex of experimental mice.methods to inject okadaic acid to mice ventriculus lateralis and to measure tau protein level,sod activity and lipid superoxide mda content of sod control group,oa injection group,oa and gastiodia elata injection solution group.results tau protein of experimental group(p＜0.05/p＞0.05),sod was lower than model group(p＜0.001) and was higher than control group(p＜0.05).conclusion gastrodia elata blume can increase sod activity and reduce tau protein expression and superoxide lipid forming in brain tissue of experimental dementia mice caused by oa.it can prevent and treat ad.

目的 观察天麻对痴呆模型大鼠海马、皮质神经元微管相关蛋白、超氧化物歧化酶和脂质过氧化物丙二醛表达的影响，探讨其治疗阿尔茨海默病(alzheimer disease，ad)的作用机制。方法用冈田酸(okadaic acid，oa)注射大鼠侧脑室造模，测定模型组、实验组、对照组海马和皮质tau蛋白、sod、mda的含量。结果实验组tau蛋白低于模型组(p＜0.001)，高于对照组(p＜0.05)；sod高于模型组(p＜0.001)和对照组(p＜0.05)；mda低于模型组(p＜0.001)，高于对照组(p＜0.05)。结论天麻可增强oa致实验性痴呆大鼠脑组织sod活性，降低mda蓄积和tau蛋白生成，具有防治阿尔茨海默病的作用。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。