蛋白

Methods Proteins existed in adult Schistosoma japonicum membrane were extracted by Triton X-100 and purified through reverse-phase high performance liquid chromatography,and the main protein peaks were then collected separately. 125 I-LDL of human plasma as the ligand, through the radioautography and radioligand binding assay, the protein which can bind human serum 125 I-LDL specifically was identified.

自人工感染家兔的肠系膜下静脉取日本血吸虫成虫，用非离子去污剂TritonX 10 0抽提出日本血吸虫成虫表膜蛋白，反相高效液相色谱进行分离纯化后，收集各主要分离蛋白峰，以12 5I标记的人血浆低密度脂蛋白作为放射性配体，经放射性自显影及放射性受体配体结合分析鉴定能与人血浆12 5I LDL特异结合的蛋白。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

The result showed that : soybean protein and corn protein which were primarily improving the capability of mulsification were selected as the appropriate protein material for protein bar, 10% of saccharose was appended and 20% of moisture was adjusted, perfect protein bar was available by extrusion .

结果表明采用乳化性大豆蛋白和乳化性玉米蛋白为蛋白原料，添加10%的蔗糖和调整含水量为20%，利用挤压膨化可得到较为理想的蛋白棒。

The central issue of this paper is the study of DNA-binding proteins interacted with DNA replication origin. The paper presented here includes there parts which are related as well as independent of each other:(1). Identification and partially purification of DNA-binding proteins interacted with eukaryotic DNA replication origin;(2) Specific binding of Hela cell proteins to Samian Virus 40 replication origin with the cruciform structure (Stem-Loop structure);(3) The study of DNA-binding protein as a tumor marker.

本文对DNA结合蛋白的研究主要包括三个即有关联，又相对独立的方面：（1）猿猴肾脏细胞复制起始区DNA结合蛋白的鉴定和部分纯化；（2）Hela细胞中DNA结合蛋白对含茎环结构的SV40复制起始区DNA的特异性结合；（3）DNA结合蛋白做为肿瘤征兆物的研究。

In order to establish a sensitive immuno-PCR assay for detecting serai anti-P53antibodies, investigate the relation of anti-P53 antibodies in patients with breast carcinoma to P53 protein expressions in tissue and clinical significances and afford a practical tool in diagnosis of breast carcinoma clinically. SaHIgG was coupled to PNCa DNA by chemical cross-linker PEI and SaHIgG-DNA probe was constructed.

本研究建立了血清抗P53蛋白抗体免疫聚合酶链反应(Immuno-polymerase chain reaction，免疫PCR)检测方法，探讨了乳腺癌患者血清中抗P53蛋白抗体与组织中P53蛋白表达之间的关系及临床意义，旨在为临床乳腺癌P53蛋白检测提供实用的替代工具。

The results showed that Shenfukang can eliminate urine protein, increase the levels of serum protein, albumin, globulin and A/G ratio, and decrease the contents of serum cholesterol, creatinine and urea nitrogen.

结果表明，肾复康可消除C-BSA肾炎模型大鼠尿蛋白，升高C-BSA肾炎模型大鼠血清总蛋白、白蛋白含量及白蛋白/球蛋白比值，降低C-BSA肾炎模型大鼠胆固醇含量、血清肌酐和尿素氮含量，对肾功能具有保护作用。

Methods: The traditional method of chronic serum sickness was modified by adding followings: one kidney removed, intraperitoneal inject LPS etc., and the effect of Shenhua Tablet on 24h proteinuria, blood biochemical indices, kidney function, pathology and immunofluorescence changes was observed compared with Fosinopril, and and the effect of Shenhua Tablet on the ED-1 positive cell number in the glomerulus, protein and mRNA expression and activation of Stat3 and protein and mRNA expression of TIMP-1 in the kidney tissue, and a-SMA positive mesangial cells were observed compared with Fosinopril using western blot, northern blot and immunohistochemistry methods.

对传统的大鼠慢性血清病肾炎模型制作方法进行以下改良：切除大鼠一侧肾脏、腹腔注射大肠杆菌内毒素、以及改尾静脉注射为腹腔注射。与西药福辛普利作对照，观察中药肾华对改良慢性血清病肾炎大鼠尿蛋白、血生化、肾功能、肾脏病理变化的影响。并通过免疫组化、Western蛋白印迹及Northern杂交方法，观察中药肾华对改良慢性血清病肾炎大鼠肾组织肾小球内炎细胞浸润、Stat3蛋白与基因表达及活化、以及其下游调控的TIMP-1蛋白与基因表达、系膜细胞增殖的影响。

Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Cortis tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.

结果①HE常规染色大体观上了解了C57BL／6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像；②获得了各种听功能密切相关的结构的成熟时间点，基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14)；③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点：④Calbindin在E16以后的螺旋神经元没有表达，但是在scarpa神经元持续表达。

Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Corti\'s tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.

结果①HE常规染色大体观上了解了C57BL／6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像；②获得了各种听功能密切相关的结构的成熟时间点，基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14)；③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点：④Calbindin在E16以后的螺旋神经元没有表达，但是在scarpa神经元持续表达。

Results: In contrast to negative expression in normal oral mucosa, survivin was expressed in 42 of 50 (84%) cases of oral squamous cell carcinoma samples. There was no significant correlation between survivin expression and age and sex. In squamous cell carcinoma tissues, the expression of Survivin gene stepped up following malignant degree, but the positive rate of Survivin was not statistically significant.

结果：Survivin蛋白在10例正常口腔粘膜组织中呈阴性，而在50例口腔鳞癌组织中有42例阳性，占84%，差异有极显著性(P.01)；COX-2蛋白在10例正常口腔粘膜组织中呈阴性，但在口腔鳞癌组织中的阳性率为86%(43/50)，差异有极显著性(P.01)；Survivin蛋白阳性率与年龄和性别无相关性，而与COX-2蛋白表达阳性呈正相关(P.05)。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。