蛋白

Clau (5, 10, 20〓) was bound to rat plasma a greater extent than clau, while in rabbit plasma the reverse was the case. By Scatchard plots using bovine serum albumin, clau had a higher equilibrium constant and the greater numbers of binding sites than its antipode.

在与大鼠血浆蛋白的结合中，clau的结合率平均为28.37±0.31（n=3，三个剂量5，10，20μM），clau的结合率平均为38.05±2.61（n=3），clau结合率低于clau；而在与兔血浆蛋白的结合中，clau的结合率平均为47.21±4.91（n=3，三个剂量），clau的结合率平均为41.43±5.21（n=3），clau的结合率略高于clau；在与牛血清白蛋白的结合中，clau的结合率也高于clau，立体选择方向与兔血浆蛋白结合相同，而与大鼠血浆蛋白结合相反，提示存在种属差异；在与系列浓度的牛血清白蛋白结合中，通过Scatchard作图法求得蛋白结合点数和平衡常数；在clau，N和K分别为90.02±35.76和5.326±1.196（n=4）；在clau，N和K分别为59.92±20.39和4.203±1.250（n=4），两者的比值分别为1.50和1.27；进一步说明clau对映体的蛋白结合存在立体选择性差异。

The female salivary gland proteins were influenced by JH Ⅲ. Treatment on d2 after feeding, 10μg dose increased SG protein level and restrained 136kD protein gene expression. Treatment on d0 after engorgement, 10μg, 50μg and 100μg doses significantly increased the d2 SG protein and 100μg dose made 35kD protein gene expression, 200μg treatment made the 136kD and 192kD proteins absent. This is the first found that JHs regulate arthropoda SG activity.

保幼激素对雌蜱唾液腺蛋白及成分有影响，吸血后2天处理，10 μg剂量使唾液腺蛋白含量明显增加，抑制136kD蛋白的基因表达；饱血当天处理，10μg、50μg和100μg显著提高饱血后2天唾液腺蛋白含量，100μg使35kD蛋白表达，200μg使136kD和192kD的蛋白缺矢；饱血当天处理，1μg和10μg剂量均能显著提高饱血后4天雌蜱唾液腺蛋白含量，高剂量（100μg）抑制136kD蛋白的表达，该结果首次报道了保幼激素对节肢动物唾液腺的调控作用。

In addition this paper,"Bio-Rad method," two parasitic wasps of the post-embryonic development the process of protein accumulation in young bees and the host Plutella xylostella in this process in vivo haemolymph protein content were measured and found that over time, was gifuensis parasitic larvae of the diamondback moth haemolymph protein content showed an upward trend in nearly a straight line, Ichneumonidae parasitic For example, in the early parasitic diamondback moth haemolymph protein content also showed the trend of slow growth, but in the mid-and late parasitic young bee about to be completed in the development of the host body when to stop feeding, a significant downward trend却呈, and significantly lower than the third day of its own protein content; on the same day of the different species of the diamondback moth parasitic wasp hemolymph protein content compared Ichneumonidae Parasitism of diamondback moth have hemolymph protein content than in the third and fourth day after the parasitic Braconidae hemolymph content to be high, but very different in the case of the fifth day, as evidenced by her side after the parasitic bee moth haemolymph protein content is cocoon of the parasitic bee hemolymph protein content of 0.7 times.

另外本文采用"Bio-Rad法"对两种寄生蜂胚后发育过程中幼蜂蛋白积累及寄主小菜蛾在此过程中体内血淋巴蛋白含量进行了测定，结果发现随时间的推移，被茧蜂寄生小菜蛾幼虫的血淋巴蛋白含量是呈近直线增加趋势，而对于姬蜂寄生来说，在寄生初期小菜蛾血淋巴蛋白含量也呈缓慢增长趋势，但在寄生中后期，幼蜂即将完成在寄主体内的发育取食停止时，却呈大幅下降趋势，且明显低于其自身第三天的蛋白含量；对同一天的被不同种寄生蜂寄生的小菜蛾血淋巴蛋白含量进行比较，姬蜂寄生过的小菜蛾血淋巴蛋白含量在第三天和第四天均比茧蜂寄生后的血淋巴含量要高，但在第五天的情况大不相同，表现为被姬蜂寄生后的小菜蛾血淋巴蛋白含量是被茧蜂寄生的血淋巴蛋白含量的0.7倍。

Based on electro-characteristic and double electric layers models, directional mechanisms of soy protein molecular are analyzed for the first time on DC heating condition. After the main factors which effect soy protein gels transparency and strength being studied on DC heating condition, the different affects on soy protein gels transparency and strength by DC heating and water bath heating are analyzed and compared, and the transparent mechanisms of soy protein gels are further explored. In addition to temperature action as water bath heating, DC heating is added as electric field directional action. As a results, heating rate is increased. Directional range degrees of soy protein molecules are raised. These advantage the increase of soy protein gels transparency and strength. Microstructures of soy protein gels forming on DC heating condition are observed by SEM and the results showed that ordered structure of transparent soy protein gels on DC heating condition is more ordered than that of transparent soy protein gels on water bath heating condition.

首次从大豆蛋白的电特性和双电层模型上分析了直流电加热条件下，大豆蛋白分子定向的机理；通过对直流电加热条件下影响大豆蛋白凝胶透明性和强度主要因素的研究，分析和比较了直流电加热与水浴加热对大豆蛋白凝胶透明性和凝胶强度的不同影响，又进一步探讨了大豆蛋白凝胶透明的机理；直流电加热除了水浴加热中的温度作用以外，又增加了直流电场的定向作用，提高了加热速率，提高了大豆蛋白分子定向排列的程度，有利于蛋白质凝胶透明性和强度的提高；利用SEM对电场下大豆蛋白凝胶微观结构的观察，进一步证明了直流电加热比水浴加热形成的透明大豆蛋白凝胶微观结构具有更高的有序性。

Peripheral plasma membrane proteins extracted from the spermatozoa from caput,corpus and cauda epididymis as well as vas deferens were probed with four lectins—Arachis hypogaea agglutinin、Canavalia ensiformis agglutinin、Dolichos biflorus agglutinin、Triticum vulgaris agglutinin.The results indicated that the 104 and 95kD AHA binding glycoproteins,and the 117、114、104、87、76、70kD ConA binding glycoproteins were synthesized and modified in the testis;the 120 and 65kD ConA binding glycoproteins,and the 54kD DBA binding glycoprotein were modified or lost during epididymal maturation;the 109kD ConA binding glycoprotein,the 74、70、45kD DBA binding glycoproteins,and the 57kD TVA binding glycoprotein were modified during epididymal maturation.

结果表明：与花生凝集素结合的104kD和95kD糖蛋白，与伴刀豆凝集素结合的117、114、104、87、76、70kD糖蛋白在睾丸中已完成了蛋白合成及修饰过程；与伴刀豆凝集素结合的120kD和65kD糖蛋白，与双花扁豆凝集素结合的54kD糖蛋白在精子附睾成熟过程中被修饰成其它种类的蛋白或被丢失；与伴刀豆凝集素结合的109kD糖蛋白，与双花扁豆凝集素结合的74、70、45kD糖蛋白，以及与麦胚凝集素结合的57kD糖蛋白是在附睾中完成其修饰过程的。

There are some isoforms of PAPs showing inhibitory ability to virus infection in various organs and at different stages of development. Three different species of the protein can be isolated from leaves of pokeweed. Proteins were precipitated with 50-80% ammonium sulfate from leaves in spring, summer and autumn respectively. Proteins were purified on SP cation-exchange then on MQ anion-exchange and finally on MS cation-exchange or on MQ anion-exchange. We found that some new pokeweed antiviral proteins separated from these leaves were different from PAP, PAPII and PAPIII.

对美洲商陆春季、夏季、秋季采集的叶片，用50-80％饱和度硫酸铵沉淀出来的蛋白样品，经过SP阳离子柱后，上MQ阴离子柱，然后把活性蛋白峰再上MS阳离子柱或MQ阴离子柱分离，从春季叶片中分离出四个不同的抗病毒蛋白峰；从夏季叶片中分离出二个不同的抗病毒蛋白峰，这两个蛋白峰与春季叶片中分离出的活性蛋白峰都能重叠上；但从秋季叶片中只分离出来一个抗病毒蛋白峰，它和春季、夏季叶片中分离出的活性蛋白峰都重叠不上。

The results showed that the water retentiveness of soybean separation protein was the best, followed by casein, and pH value, metal ions such as Ca2+, Mg2+, Zn2+, and Na+, etc. had some effects on the water retentiveness, with effects differed greatly among these factors; the emul...

结果表明：大豆分离蛋白的持水性能较好，其次为酪蛋白，pH值、金属离子Ca2+、Mg2+、Zn2+、Na+等对持水性有一定的影响，呈现较大差异；大豆分离蛋白的乳化性能较好，其次为酪蛋白、乳清蛋白，动物蛋白较差，在影响乳化性能中金属离子以多价离子为主要因素；大豆蛋白的热稳定性较差，酪蛋白和动物蛋白较好。

In addition this paper,"Bio-Rad method," two parasitic wasps of the post-embryonic development the process of protein accumulation in young bees and the host Plutella xylostella in this process in vivo haemolymph protein content were measured and found that over time, was gifuensis parasitic larvae of the diamondback moth haemolymph protein content showed an upward trend in nearly a straight line, Ichneumonidae parasitic For example, in the early parasitic diamondback moth haemolymph protein content also showed the trend of slow growth, but in the mid-and late parasitic young bee about to be completed in the development of the host body when to stop feeding, a significant downward trend却呈, and significantly lower than the third day of its own protein content; on the same day of the different species of the diamondback moth parasitic wasp hemolymph protein content compared Ichneumonidae Parasitism of diamondback moth have hemolymph protein content than in the third and fourth day after the parasitic Braconidae hemolymph content to be high, but very different in the case of the fifth day, as evidenced by her side after the parasitic bee moth haemolymph protein content is cocoon of the parasitic bee hemolymph protein content of 0.7 times.

另外本文采用&Bio-Rad法&对两种寄生蜂胚后发育过程中幼蜂蛋白积累及寄主小菜蛾在此过程中体内血淋巴蛋白含量进行了测定，结果发现随时间的推移，被茧蜂寄生小菜蛾幼虫的血淋巴蛋白含量是呈近直线增加趋势，而对于姬蜂寄生来说，在寄生初期小菜蛾血淋巴蛋白含量也呈缓慢增长趋势，但在寄生中后期，幼蜂即将完成在寄主体内的发育取食停止时，却呈大幅下降趋势，且明显低于其自身第三天的蛋白含量；对同一天的被不同种寄生蜂寄生的小菜蛾血淋巴蛋白含量进行比较，姬蜂寄生过的小菜蛾血淋巴蛋白含量在第三天和第四天均比茧蜂寄生后的血淋巴含量要高，但在第五天的情况大不相同，表现为被姬蜂寄生后的小菜蛾血淋巴蛋白含量是被茧蜂寄生的血淋巴蛋白含量的0.7倍。

The identified proteins are involved in a variety of cellular process including several zinc finger proteins relevant to transcription regulation, such as zinc finger 198, 263, 14, 224, zf6, novel protein similar to transcriptional represser CTCF, and kruppel-like zinc finger protein; two members of the ADAMs (a disintegrin and metalloprotease domain) family; two members of integrin family; several proteins involved in the signal transduction, cell-cycle control, chromatin remodeling and transcription repression; and also some proteins of cell skeleton and some with unknown functions.

鉴定出 的蛋白包括多个与转录调控相关的锌指蛋白，如锌指蛋白198、263、14、224、zf6、转录抑制因子cTcF样蛋白、幻肚pple样锌指蛋白等：两个含金属蛋白酶结构域和整合素结合结构域的家族成员ADAM28和ADAM17；两个整合素家族成员pZ整合素和含十个EGF样结构域的整合素；与细胞信号转导通路有关的蛋白；与细胞周期调控有关的蛋白；与染色体重塑和基因转录抑制有关的蛋白；细胞骨架蛋白以及其他功能未明的蛋白等。

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制，酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力，酶蛋白亚基间的相互作用，酶蛋白的折叠以及内运动状态与酶活力的关系，酶蛋白发挥功能时的结构特性；运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性，热稳定性机制；确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位；确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构；确定了一个新型的染色质蛋白Cren7的溶液三维结构；正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失