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Under two levels of earing fertilization (N0 and N2), compared with 25℃ treatment, heat stress (35℃) accelerated the grain filling and resulted in less grain weight, and also reduced the two glutelin subunits content of both fertilization treatments 12.1% and 5.7% respectively, and reduced the prolamin content of them 6.7% and 4.9% respectively. Simultaneously, the polypeptides above 57 kD was increased 18.8% and 11.7% respectively, and grain crude protein content was also increased 0.56% and 0.35% respectively.

在N0和N2两个穗肥水平下,35℃高温处理均提高了子粒灌浆速度,明显降低了子粒单粒重;35℃高温处理还使谷蛋白的两个亚基的含量较25℃处理分别降低12.1%和5.7%,使醇溶蛋白含量分别降低6.7%和4.9%,同时却使谷蛋白组分中57kD及以上多肽的积累分别增加18.8%和11.7%,且子粒的粗蛋白含量也分别增加0.56%和0.35%。

Western blot and the immunoblot results indicate that the antibodies not only can react with silicon-binding proteins of rice, but also can cross react with the proteins of other silicon-accumulated graminaceous plants, while it does not react with the proteins of non-silicon accumulated dicotyledonous plants and BSA. These findings indicate that the homologous proteins of SBP117 are widely existed in the graminaceous plants.

蛋白质印迹和免疫印迹检测表明,SBP117的抗体不仅能识别水稻硅结合蛋白,而且与其他累积硅的禾本科植物中提取的硅结合蛋白有交叉反应,但它不识别不累积硅的双子叶植物番茄叶中的蛋白质以及牛血清蛋白,说明与SBP117同源的硅结合蛋白广泛存在于禾本科植物之中。

The GFAP is a specific marker of astrocyte, its expression is more higher in the activity astrocyte, and finally the GFAP become the main composition of scar formations. The S -100 is a kind of acidity, dissolubilites, low molecular quantity and calcium hydronium conjugated protein, and it is mainly existed in neuroglial cell and schwann cell. It can promote the growth of axon, glial hyper-plasia and nerve divide and calcium's stability inside of cell, thus regulatin g the shape and metabolism of astrocyte . The quantity of S -100 protein and the degree of ischemia have direct proportion .

胶质纤维酸性蛋白是星形胶质细胞的特异性标记物,在活性星形胶质细胞中GFAP的表达相对更高,且最后GFAP成为胶质疤痕的主要成份。S-100蛋白是一种酸性、可溶性、低分子量的钙离子结合蛋白,主要存在于胶质细胞和雪旺细胞中,它可促进轴突生长、胶质增生、神经分化和细胞内钙的稳定,从而调节星形胶质细胞的形态和代谢。S-100蛋白与缺血的程度是成正比的。

Change of expression of AQP4 protein was the same like the change of expression of AQP4 mRNA. We observed it in model group was statisticsly higher than that of control group. The quantity of AQP4 protein in intervenor groups was remarkably lower than that in model group.But the quantity of AQP4 protein in intervenor groups obviously increased compared with control group.

5AQP4蛋白表达与AQP4 mRNA表达呈现相同变化趋势,与对照组相比较,模型组各时间点的AQP4蛋白的含量均显著升高,以造模后第24h最明显;AQP4干预组和HO-1干预组在各时间点的AQP4蛋白的含量均显著高于对照组,但明显低于模型组AQP4蛋白的含量。

Results The GRK5 protein level in Parkinson's disease α-synuclein transgenic mice was increased to different degrees compared with that in non-transgenic littermate control mice, and the protein and mRNA expressions of GRK5 in 9-month old A53T mutant mice were higher than those in NLC mice, but there were no significant changes in the 3 month and 6 month old A53T mutant mice.

结果 各组帕金森病α-synuclein转基因小鼠与阴性对照小鼠相比,GRK5蛋白表达水平均有不同程度的增加,并且随着转入的h-α-syn蛋白表达水平的高低而有所变化。3月龄和6月龄帕金森病转基因模型小鼠与同月龄阴性对照组小鼠相比,GRK5的mRNA和蛋白水平没有变化;而9月龄帕金森病转基因模型小鼠与同月龄阴性对照组小鼠相比,GRK5的mRNA和蛋白水平都有所增加。

The result showed that crude protein content in food residue could be increased by compound culture of microzyme and mildew ,and crude protein content rose with reaction time increasing.

结果表明:酵母菌和霉菌联合作用于食物垃圾可以提高其粗蛋白含量,且粗蛋白含量随反应时间的延长而增加;康宁木霉3.2774与发酵水解液废液酵母2.570联合作用时效果最好,反应48h后,食物垃圾样品的粗蛋白含量增加了5.09%,相对增量达34.56%,因此可以选择这2种菌种作为食物垃圾固态发酵生产微生物蛋白饲料的优选复合菌种。

There was a stastical difference of the expression of hTRT protein among well differentiated adenocarcinoma, poor differentiated adenocarcinoma and mucoid carcinoma. And there was a highly significant positive correlation between the expression of hTRT mRNA and hTRT protein. However, the expression of hTRT mRNA and its protein in GC were not related with other clinicopathological parameters including gender, age, location and size of neoplasm, wall invasion, lymph node metastasis and clinical stage.

结果:1。在53例GC中hTRTmRNA及hTRT蛋白表达均显著高于癌旁组织,hTRT蛋白表达在粘液癌与高分化腺癌和低分化腺癌间表达有显著性差异,hTRTmRNA与hTRT蛋白的表达呈显著正相关,而hTRTmRNA、hTRT蛋白的表达与各临床病理参数包括性别、年龄、肿瘤大小、部位、淋巴结转移、浸润深度及临床分期均无相关性; 2。

The recombinant baculovirus was transfected into sf-9 cells by CellFectin reagent. SDS-PAGE and Western-blot analysis showed that the expressed protein was 37ku in molecular mass and exhibited specific immunological activity. These results provide foundation for development of a subunit vaccine with the expressed σC protein against Muscovy duck reovirus infection.

在脂质体介导下将rBacmid-σC转染sf-9昆虫细胞,获得重组杆状病毒rBV-σC;SDS-PAGE和Western-blot分析表明,在sf-9细胞中表达了分子质量约37ku的σC蛋白,该蛋白能与原核表达的σC蛋白免疫小鼠血清发生特异性免疫反应,这为以表达的σC蛋白为抗原的亚单位疫苗的研制奠定了基础。

In search of databases, the deduced products of sanP, sanQ, sanR, sanS, sanT and sanU show highest similarity to those of nikP2, nikQ, nikR, nikS, nikT and nikU of S. tendae respectively. In comparison with the proteins of identified function, it is indicated that sanP encodes a thioesterase, sanQ encodes a cytochrome P450, sanR encodes a uracil phosphoribosyltransferase, sanS encodes a carboxylase, sanT encodes a histidinol-phosphate aminotransferase and sanU encodes a mutase.

蛋白数据库中比较结果表明,sanP、sanQ、sanR、sanS、sanT和sanU基因编码的蛋白分别和唐德链霉菌的尼可霉素生物合成基因nikP2、nikQ、nikR、nikS、nikT、nikU六个基因编码的蛋白同源性最高;根据和已知功能蛋白的比较,推测sanP基因编码的是硫酯酶,sanQ基因编码的是细胞色素P450,sanR基因编码的是尿嘧啶磷酸核糖转移酶,sanS基因编码的是羧化酶,sanT基因编码的是组氨醇磷酸氨基转移酶,sanU基因编码的是一种变位酶。

Myofibril fragmentation index, contents of collagen (total collagen, heat soluble collagen, heat insoluble collagen) and muscle fiber diameter were used to evaluate the histology characteristics, and meat tenderness of Chinese mitten crab from 4 different locations was analyzed by using the method of combination index.

中文摘要:摘要:采用肌原纤维小片化指数、胶原蛋白(总胶原蛋白、热溶性胶原蛋白、热残留胶原蛋白)和肌纤维直径3种评定方法,分别测定了4地区中华绒螯蟹肌肉的组织特性,并初步探讨了采用综合指数法比较肉质的嫩度。

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