蛋白

Objective: Establish mucins' protein chip and test the serum levels of mucinl,...

目的：应用粘蛋白抗体制备粘蛋白检测用蛋白芯片，并对胃肠道癌症患者血清进行粘蛋白MUC1、MUC2、MUC5AC及CEA的检测，与健康人群进行对照，以揭示粘蛋白在胃肠道癌症患者血清中的表达规律及可能预示的临床意义，探讨该芯片用于胃肠道癌症筛查和血清学诊断的可行性。

And objective parameters (texturized degree, color, hardness, springiness, chewing, water absorption, yield, etc.) were investigated in the high moisture soy protein extrusion process, and the statistical models of system and objective parameters were built, using the step-by-step regression analysis; A comprehensive evaluation of the products, and the process optimization of high moisture extrusion were obtained,using factor analysis; Secondly, residence time distribution and soy isoflavones losses kinetics were studied in the high moisture extrusion; Finally, chemical bonding process, the micro-structure and protein secondary structure changes were investigated in the texturization of soy protein, then the mechanism assumptions of texturization soy protein by moisture extrusion were proposed.

本文对大豆蛋白高水分挤压组织化技术和机理进行了研究，研究内容包括：首先采用系统分析法，研究了大豆蛋白高水分挤压过程中，挤压机操作参数(螺杆转速、物料水分、喂料速度和机筒温度)对系统参数(系统压力、扭矩、单位机械能等)、目标参数(组织化度、色泽、硬度、弹性、咀嚼性、吸水率、产量等)的影响规律，建立了各因变参数的统计模型；采用因子分析法对产品进行了综合评价，并对高水分挤压组织化工艺进行了优化；其次，研究了大豆蛋白高水分挤压中的停留时间分布以及大豆异黄酮的损失动力学；最后，研究了大豆蛋白组织化过程中化学键、微观结构和蛋白质二级结构的变化规律，提出了大豆蛋白高水分挤压组织化的机理假设。

In mammals the Notch gene family comprise four related genes, Notch1-4, as the Notch ligands that encode transmembrane proteins. Ligand-receptor interaction mediated Notch activation ultimately leads to the conversion of effectors-CSL CBF1, Su(H, Lag-1 proteins, from repressors to transcriptional activators, and subsequent up-regulation of downstream targets.

在哺乳动物中，Notch基因家族包含了4个相关的基因，分别为Notch-1至-4，和Notch受质蛋白一样能被转译成膜蛋白；受质-受体间相互作用所引起Notch受体蛋白的活化会促使作用蛋白-CSL CBF1， Su(H， Lag-1 蛋白扮演抑制分子或者是转录活化分子上的角色改变，并且进一步地正向调节下游的标的基因。

BST16 was also proved to encode gene of PSII lOkD protein by protein prosite and conserve domain analysis. The protein encoded by BST16 contained a conserve domain PsbR of PSII lOkD protein from 48 to 140 and its carboxy terminal had an ADH_SHORT prosite of Short-chain dehydrogenases/reductases family signature from 94 to 122, which showed the protein encoded by BST16 would be a reductases.

对该基因可读框架编码的蛋白进行功能位点和结构功能域的分析，同样证明了该基因为PSⅡ 10kD蛋白编码基因，其氨基酸摘要序列 48刁位含有一个保守的光合系统＊ 10kD蛋白结构域 PSbR，在蛋白的猿基端有一个保守的短链脱氢酶／还原酶家族特征序列ADHSHORT（Short－chain dehydrogenases／reductases family signature），位于94－122位，说明了该蛋白可能具有还原酶活性。

The difference in the above indices between chalky and non chalky milled rice in Zhaiyeqing 8 was not remarkable. Crude protein content of chalky milled rice in Zhaiyeqing 8 was significantly lower than that of non-chalky rice, hot there was not remarkable changes between chalky and non chalky milled rice in Gangyou 527. Glutelin content fell significantly, albumin, globulin, prolamine and lysine contents did not change remarkably in chalky milled rice compared with non chalky milled rice in the two varieties.

窄叶青8号垩白米与非垩白米的上述各指标间无显著差异窄叶青8号垩白米的粗蛋白含量显著低于非垩白米，而冈优527垩白米的粗蛋白含量与非垩白米相比无显著变化2个品种垩白米的清蛋白、球蛋白、醇溶蛋白、赖氨酸含量与非垩白米间无显著差异，但谷蛋白含量显著低于非垩白米。

Five expressed genes contributed to the flowering process and embryonic development such as prolamine, gene for allergenic protein, chalcone synthase, putative peptide methionine sulfoxide reductase and acyl carrier protein Ⅱ gene were chosen to hybridize with a 1510-RNA-blot (time-point phenotypes) array. The 1510 RNA blots were prepared into an RNA array for the purpose of conferring and validating global transcriptional profiles on booting, flowering and filling quantitatively and qualitatively at a statistic level. The results verified: Prolamine and allergenic protein were high-expressed in filling grains after being regulated by development, Chalcone synthase and methionine sulfoxide reductase were high-expressed in the leavies induced by light, in the root stressed for nitrogen deficiency.

为了全局性分析基因表达谱以及进一步验证它们与开花过程的相关性，选取参与水稻开花过程和胚胎发育过程的基因：查尔酮合酶、酰基载体蛋白Ⅱ、醇溶蛋白、S-腺苷基甲硫氨酸还原酶、过敏反应蛋白等为探针，与1500个水稻RNA斑点阵列进行杂交时，结果证实：醇溶蛋白和过敏反应蛋白受发育调节在乳熟成穗中高表达，查尔酮合酶和S-腺苷基甲硫氨酸还原酶在叶片中受光诱导、在根中受缺氮胁迫高表达。

The 94-kDa GLase activity was Ca++ dependent, showed maximal activity in the pH 6-8, their activity was inhibited in the presence of 1,10-phenanthroline, and their proenzymes were cleaved and activated after incubation with organomercurial compounds and trypsin.

72kDa蛋白脢，出现於所有检体，包括未被感染的老鼠，94kDa蛋白脢只出现於广东住血线虫感染老鼠脑部。94kDa蛋白脢活性是一种钙离子依赖性，在pH值6-8时具最大活性，在 1，10-phenanthroline存在下，其活性受到抑制，此蛋白酵素元会被有机汞类及胰蛋白脢切断并活化。

The genome of wheat rosette stunt virus, a plant rhabdovirus, is a single negative strand RNA.

小麦丛矮病毒是在中国发现的一种植物弹状病毒，病毒基因组是由一条单链负链RNA组成并编码 5种病毒结构蛋白质：表面糖蛋白G、膜基质蛋白M、核衣壳蛋白N、大蛋白L和所谓非结构蛋白NS。

According to result of group I, II, HI, relationship of dry matter intake digestible energy intake , crude protein intake , ruminal degradable protein or ruminal undegradable protein with average daily gain of stall-feeding growing Boer goats were analyzed.

根据Ⅰ、Ⅱ、Ⅲ组试验数据得出波尔山羊干物质采食量、粗蛋白质采食量、瘤胃降解蛋白、瘤胃非降解蛋白及消化能采食量与羔羊日增重的回归关系如下： DMI=2.540△W+659.7(r=0.953，p.05) CPI=0.70△W+40.492(r=0.955，p.05) RDPI=0.213△W+65.06(r=0.856，p.05) UDPI=0.488△W-24.56(r=0.984，p.05) DEI=68.56△W+1074.9(r=0.924，p.05)根据回归方程，本研究提出了波尔山羊(3～5月龄)日增重0～250g的每日每只营养需要建议参数：即当预期日增重分别为0g、50g、100g、150g、200g、250g时，消化能日供给量分别为1.08MJ、4.50M3、7.93M5、11.36MJ、14.79MJ、18.21MJ，粗蛋白日供给量分别为40g、75g、110g、145g、180g、215g，瘤胃降解蛋白日供给量分别为409、759、869、979、1089、1189，瘤胃非降解蛋白日供给量分别为09、09、249、489、729、979。

METHODS: The subcutaneous adipose tissue was obtained from adult New Zealand rabbits under aseptic condition, and cultured in vitro with collagenase digestion. All cells were divided into 3 groups: in the BMP-2 group, cells were cultured with medium containing 0.1 g/L vitamin C, 10 mmol/Lβ-sodium glycerophosphate and 10μg/L BMP-2 for 10 minutes, followed by 4-14 days inoculation with density of 18×104 cells per pore. In the BMP-7 group, cells were cultured with BMP-7 with the same methods as BMP-2 group. The cells were cultured with simple culture medium in the control group.

无菌切取成年新西兰大白兔皮下脂肪，胶原酶消化法体外分离培养脂肪干细胞，分为3组：骨形成蛋白2组加入含0.1 g/L维生素C、10 mmol/L β-甘油磷酸钠、10 μg/L骨形成蛋白2的诱导培养基培育15 min，然后按18×104个细胞/孔接种，再培育4~14 d；骨形成蛋白7组培养方法基本相同，仅将骨形成蛋白2更换为骨形成蛋白7；对照组同法加入单纯培养基进行培育。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。