蛋白

Extraction of the existing animal protein, plant protein extract, pharmaceutical-grade peptone, protein, small peptides and small protein peptide complex modern production lines, raw materials, the use of high efficiency, no pollution, the main product have: 1, protein categories: including animal protein and plant protein, animal protein which include the skin collagen, bone collagen protein, beef protein, beef extract, casein protein.

现有动物蛋白提取、植物蛋白提取、医药级蛋白胨、蛋白小肽和复合蛋白小肽等现代生产线，原材料使用效率高、无污染，主要产品有：1、蛋白质类：包括动物蛋白和植物蛋白，其中动物蛋白包括皮胶原蛋白、骨胶原蛋白、牛肉蛋白、牛肉浸膏、酪蛋白等。

Now we have known that SARS-CoV encoded 11 varietal proteins. They have 4 constitutive proteins: spike protein, membrane protein, envelope protein and nucleocapsid protein. Spike protein trimer form spike and exsertion? virus peplos on the surface. Spike protein is archi-surface antigen component of virus, it has acceptor binding and membrane fusion activity. Spike protein has important effect at tissue tropism, cell-fusion and toxicity. Nucleocapsid protein can bind genome RNA to shape neucleocapsid. Membrane protein and envelope protein are necessary constituent to shape virus peplos. Spike protein is I-type membrane glycoprotein, it mediate virus binding with host cell epimembranal acceptor and to fuse host cell membrane.

SARS-CoV属于巢状病毒目、冠状病毒科，为单正链RNA病毒，基因组全长约29.7kb，已知编码11种蛋白，其中包括4种结构蛋白，分别是刺突蛋白、包膜蛋白、小包膜蛋白和核蛋白或核壳体蛋白。S蛋白三聚体形成突起，伸出包膜表面。S蛋白是病毒的主要表面抗原成分，具有受体结合和膜融合活性，在组织嗜性、细胞融合和毒力等方面起重要作用。N蛋白与基因组RNA结合形成核衣壳。M蛋白和E蛋白是包膜形成所必需的成分。

In its protein has the albumin,谷蛋白, mellowly dissolves theprotein, the amino acid and so on, always tonic which prolongs thelife by our country people achievement.

其蛋白质中有白蛋白、谷蛋白、醇溶蛋白、氨基酸等，历来被我国人民作为延年益寿的补品。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Finally confirmed that,The thighbone and the osteoporosis morbidity is possibly connected protein 6, respectively are: The lactoferrin light chain, membrane association protein A3, the enolase, ATP gather the enzyme, the acetyl coenzyme A reductases, the myo calcium protein; The lumbar vertebra and the osteoporosis morbidity is possibly connected protein 5, respectively are: The actin, the keratin, the enolase, ATP gather the enzyme, the myosin; The thighbone and the strong bone valuable curative effect is possibly connected protein 9, respectively are: The enolase, ATP gather the enzyme, the myo-calcium protein, the creatine activating enzyme isozyme, the phosphoglyceric acid change flavor the enzyme, the myosin, the lactoferrin light chain, the pyruvic acid activating enzyme isozyme, the crown protein; The lumbar vertebra and the strong bone valuable curative effect are possibly connected protein 8, respectively are: The carbonic anhydrase, the actin, the αB-crystal protein, 3-phospho-glycerol aldehyde oxidase, the serum albumin, ATP gather the enzyme, the myosin, the enolase.

最后确认：股骨与骨质疏松发病可能相关的蛋白6个，分别为：乳铁蛋白轻链、膜联蛋白A3、烯醇化酶、ATP合酶、乙酰辅酶A还原酶、肌钙蛋白；腰椎与骨质疏松发病可能相关的蛋白5个，分别为：肌动蛋白、角蛋白、烯醇化酶、ATP合酶、肌球蛋白；股骨与强骨宝疗效可能相关的蛋白9个，分别为：烯醇化酶、ATP合酶、肌钙蛋白、肌酸激酶同工酶、磷酸甘油酸变味酶、肌球蛋白、乳铁蛋白轻链、丙酮酸激酶同工酶、冠蛋白；腰椎与强骨宝疗效可能相关的蛋白8个，分别为：碳酸酐酶、肌动蛋白、αB-晶体蛋白、3-磷酸甘油醛脱氢酶、血清白蛋白、ATP合酶、肌球蛋白、烯醇化酶。

In this study, 105 TDFs were in silico mapped in the rice high-density linkage map. Nineteen TDFs were mapped to the quantitative trait loci regions for root growth under water-limited conditions in, at least, two of three rice populations derived from three crosses with the same parent of Azucena (Bala×Azucena, IR64×Azucena and IR1552×Azucena). Four of 19 genes (T37, L16, T17 and T7) were mapped based a RIL population of IR1552×Azucena by southern blot analysis. Five genes encode putative or hypothetical protein. Other 14 genes were similar with known genes in databases including expansin (OsEXP2), late embryogenesis-abundant gene , an SR-related protein essential for spliceosome assembly (SART1), autophagocytosis protein , bHLH protein, fruit-ripening protein similar to ASR, nickel-binding protein 2A, DNA-binding protein, pyruvate dehydrogenase kinase , stomatin-like protein, SR1 induced by sucrose starvation, vacuolar protein sorting protein (VSP33a), gibberellin action negative regulator and retroelement.

根据核苷酸序列将105个基因电子定位到水稻的高密度连锁图谱上，其中19个差异表达基因定位在Bala×Azucena、IR64×Azucena和IR1552×Azucena中至少两个群体共同的与根生长相关的QTLs区间，并用Southern杂交将其中的4个定位到IR1552×Azucena群体遗传连锁图谱相应的位点上。19个基因中的5个编码推断的未知功能蛋白质，其余14个编码已知功能蛋白，分别为膨胀素（Os-EXP2）、胚胎后期丰富蛋白、剪接体安装必需的SR相关蛋白（SART1）、自吞噬蛋白、碱性螺旋-环-螺旋转录因子、与ASR相似的果实成熟蛋白、镍结合蛋白、DNA结合蛋白、丙酮酸脱氢酶激酶、stomatins类蛋白、蔗糖调节蛋白SR1、液泡蛋白分类蛋白（VSP33a）、GA负调节因子、逆转座元件。

The main components in soy protein isolate are 7S and 11S globulins. Studiesof papain on 7S, 11S and mixture of the two proteins suggested that 11S is themain component in SPI to form coagulation. 7S coagulation required higher papainaddition than 11S coagulation, while 11S gel's elastic modular was higher than7S gel. The elasticity of mixture depended on the proportion of 11S. Two proteinases were separated from papain by SP-sepharose cation-exchangechromatography.

首先分离出大豆蛋白中的主要组分7S和11S蛋白，将木瓜蛋白酶分别作用于两种蛋白的溶液和不同7S/11S比例的蛋白溶液，结果表明11S蛋白是大豆蛋白中形成酶促凝胶的主要组分；7S蛋白形成凝胶所用的酶浓度高于11S蛋白所用的酶浓度，但11S蛋白形成的凝胶的弹性强度却远高于7S蛋白；在两种蛋白的混合溶液中11S蛋白的比例对凝胶的弹性强度起决定作用。

In this experiment, a new method was used to purify the toxin protein produced by Verticillium dahliae. The supernatant of fungus culture was frozen and dried with Lyophilizer first, and then dialysed by Dialysis Membranes (MWCO 1000) after dissolved in distilled water. This method can eliminate the salt and sucrose in culture medium and reserve the protein component almost completely. VD-toxin were analysed using sodium dodecyl sulfate polyacrylamide gel analysis. The results indicated that the protein components were very complex, and included glycoprotein within 35.8kDa-83.2kDa. Furthermore, treatments on glycoproteins with high temperature, conA and proteinase alleviated, not abolished, their activities.NO and H_2O_2 production were assayed in two cultivars of cotton cells which were treated with VD-toxin.

本实验首先确定了用冷冻浓缩后透析的方法初步提纯棉花黄萎病菌毒素，该方法能有效去除粗提毒素中的蔗糖和盐离子并能最大程度的保留黄萎病菌分泌的蛋白成分；对毒素蛋白成分鉴定结果表明蛋白条带多，每种蛋白的含量少，小分子量蛋白含量多，糖蛋白染色结果显示，毒素蛋白中分子量在35.8kDa-83.2kDa之间的蛋白大多数为糖蛋白，分子量小于35.8kDa的蛋白多为非糖蛋白；性质鉴定结果显示，毒素中有活性的蛋白成分具有部分可耐高温高压，且毒素蛋白中的蛋白成分和糖基成分都具有致萎活性。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失