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蛋氨酸

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In the first part, chemical synthesis, including by acraldehyde, malonic ester, ami no lactone and others were discussed and synthesis by hydantoin was emphasized.

第一部分讨论了DL-蛋氨酸及其衍生物的化学合成,包括丙烯醛法、丙二酸酯法,氨基内酯法等,并着重介绍了海因法。

Methods Normal group,HepG2 model group,groups of HepG2 cells treated with compound methionine and choline bitartrate at different concentrations,L02 model group,and model groups of L02 cells treated with drug at different concentratrions were set up.

目的 对比油酸诱导肝癌细胞株HepG2、正常肝细胞株L02建立的肝细胞脂肪变性模型,通过检验复方蛋氨酸胆碱的防治作用,寻找简便、稳定的体外药物筛选模型。

Four nitrogen sources were used in the experiments, i.e. urea, casein, lysine and methionine.

主要选用了四种氮源:尿素、酪蛋白、赖氨酸和蛋氨酸

We detemined the crystal structures of the editingdomain of E. coli LeuRS in both apo form and complexes withmethionine and isoleucine, respectively.

我们解析了大肠杆菌亮氨酰-tRNA合成酶编校结构域本身和其分别与蛋氨酸和异亮氨酸复合物的晶体结构。

An amino acid screen of blood and urine shows elevated methionine and homocysteine levels.

一个氨基酸筛选血液和尿液中显示高架蛋氨酸和半胱胺酸的水平。

The identified proteins were divided into two following categories: one is regulatory proteins, for example, caffeic acid 3-O-methyltransferas, HSP70, glyoxalase I , actin, and so on. They are involved in protein biosynthesis and processing, cellular structural organization and stress responses. The other is function proteins, for instance, ascorbate peroxidase, cytoplasmic malate dehydrogenase, cytosolic phosphoglycerate kinase, S-adenosyl methionine synthetase, ATP synthase, and so on. They are involved in photosynthesis, photorespiration, redox homeostasis, and metabolisms.

通过分析可以将这些差异蛋白分为两大类:第一类是调节性蛋白,如咖啡酸三氧转甲基酶,热激蛋白70,醛酮变位酶I,肌动蛋白等,它们主要参与蛋白质的合成和加工、胁迫响应、细胞骨架稳定等过程;第二类是功能性蛋白,如抗坏血酸过氧化物酶,细胞质苹果酸脱氢酶,细胞质磷酸甘油酸激酶,S-腺苷蛋氨酸合成酶,ATP合酶等,它们则参与氧化还原平衡、光合作用、光呼吸和各种代谢等过程。

S-adenosylmethionine is a naturally occurring molecule, which widely distributes throughout tissues and fluids. It has been one of the very important pharmaceuticals because of its essential role in transmethylation, transsulphuration and transaminopropylation.

S-腺苷蛋氨酸是一种广泛存在于各种组织和细胞中的生物小分子,具有转甲基、转硫和转氨丙基等重要生理作用,已成为治疗肝损伤、胆汁郁积和抑郁症等疾病的重要药物。

With chicken farming maggot, maggot breeding earthworm manure, earthworm dung plant grass, fly maggots, earthworms, grass used to raise chickens, produced by environmentally friendly eco green chicken meat is not only more delicious, and nutrients become more abundant, rich required by the human body with various vitamins, protein, amino acid, lysine, methionine and other nutrients, Chicken Egg yellow up to 11 degrees more than ordinary for the 5-6 Yellow Egg, egg white more thick, mouthfeel more sun are well received by consumers.

用鸡粪养殖蝇蛆,蝇蛆粪养殖蚯蚓,蚯蚓粪种植牧草,蝇蛆、蚯蚓、牧草用来养鸡,生产出来的绿色无污染生态土鸡不仅肉质更加鲜美,而且营养成份更加丰富,富含人体所需要的各种维生素、蛋白质、氨基酸、赖氨酸、蛋氨酸等多种营养成份,土鸡蛋蛋黄色度达11以上,而普通蛋蛋黄为5-6,蛋清更加浓稠,口感更加淳正,深受消费者的欢迎。

By carefully manipulating the balance of amino acids, both lifespan and fertility were maximised.

而一种特别的胺基酸—蛋氨酸—可以在不影响生育的情况下延长寿命。

Results were shown as followings:(1) Sodium selenite at 0~2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5~2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下:(1)0~2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力;而对SMMC-7721细胞的作用不显著;(2)该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度;而对SMMC-7721细胞的作用均不显著;(3)高浓度硒(5μmol/L以上)显著性抑制L-02细胞生长、致细胞过氧化,灯盏花素能拮抗硒所致过氧化、降低硒毒性;(4)0.5~2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧,同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著;(5)富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白,其结构和功能有待研究;(6)通过已有的含hTERT基因质粒,成功构建hTERT反义RNA表达质粒,转染SMMC-7721细胞后对其生长具有抑制作用。

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