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Our study designed two pairs of specific primers and cloned two molecular weight of 63ku from Schizaphis graminum, and Rhopalosiphum maize utilizing PCR technique and have done the prokaryotic expression, the main results are as follows:(1)Amplified the Buchnera groEL of Schizaphis graminum, and Rhopalosiphum maize Yangling Biotype at first time all over the world ,and sequenced the full lenghth gene.

本研究设计了特异性引物,利用PCR技术从玉米蚜和麦二叉蚜中克隆出蚜虫Buchnera groEL基因并进行原核表达研究,主要结果如下:(1)从杨凌生物型玉米蚜和麦二叉蚜中在国内外首先扩增出Buchnera groEL基因,测定了全长基因序列,并对相关的同源基因核苷酸进行了比较。

The relationship between aphid arid Buchnera is a typical mutualism.

蚜虫-巴克纳氏菌之间是一种典型的互利共生关系,两者相互依存,缺少一方,另一方便不能生存。

To further explore the phylogenetic relationships between the aphid Myzus persicae and its endosymbiotic bacterium Buchnera, COⅠ/COⅡ, 12S/16S rRNA and EF-l alpha genes in four populations of M. persicae were amplified. Through phylogenetic trees constructed by the COⅠ/COⅡ, 12S/16S rRNA and EF-l alpha genes in M.

为进一步明确桃蚜与其内共生菌Buchnera之间系统进化关系,参照前人研究蚜虫系统进化所用的COⅠ/COⅡ、12S/16S rRNA、EF-1 alpha的3对引物,对桃蚜的COⅠ/COⅡ、12S/16S rRNA、EF-1 alpha基因进行了扩增,并构建了桃蚜COⅠ/COⅡ、12S/16S rRNA、EF-1 alpha基因序列系统进化树。

Bunchy top disease can spread from plant to plant and is also spread by aphids.

束顶病能从一颗植物传播到另外一棵植物,而且也能通过蚜虫传播。

Bunchy top disease can spread from plant to plant and is also spread by aphids.

束顶病能从一棵植株传到另一棵植株,而且也能通过蚜虫传播。

Bunchy top disease can spread from plant to plant and is also spread by aphids.

聚头病可以从植物传播到植物也可以通过蚜虫传播。

Bunchy top disease can spread from plant to plant and is also spread by aphids.

束顶病会在植株之间传播,也会通过蚜虫传播。

Bunchy top disease can spread from plant to plant and is also spread by aphids.

集束病可以在植株间传播并且可以通过蚜虫传播。

Bunchy top disease can spread from plant to plant and is also spread by aphids.

束顶病除了植株间相互感染外,还能通过一种蚜虫传播。

Adults and larvae are a variety of aphids, such as food canarium.

成虫和幼虫均以多种蚜虫、木虱等为食。

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