藜芦碱

The effects of phenytoin (2.5, 5, 10 and l5 μmol/L) and gabapentin (2.5, 5 and l0μ mol/L) on the epileptiform activity were observed under the volt age- clamp configuration. and the current changes for 1 hour in CA I neurons was also observed. Results Nine-sixteen minutes after veratridine perfusion, the huge.

分别在电压钳模式下观察2.5、5、10、15μmol/L苯妥英和2.5、5、10μmol/L加巴喷丁对这种癫痫活动的影响；测定藜芦碱灌流后CA1锥体神经元Ih电流的变化规律。

However, the role of inactivation gate of sodium channel is not clear. In present study, single fibre recording in vivo from dorsal root on chronically compressed DRG model was used. We analyzed the characters of ISI series of oscillation firing of type A neurons induced by veratridine, an inhibitor of inactivation gate of sodium channel.

本研究在大鼠背根节慢性压迫模型上，利用在体单纤维记录方法，观察与分析Na通道失活门抑制剂藜芦碱引起受损背根节A类神经元放电ISI序列发生的变化特征，为了解Na通道失活门与放电型式的关系以及进一步探索放电时间型式与疼痛的关系奠定基础。

Low-dosage veratridine may induce runs of PDS like epileptiform activities on rat CA1 pyramidal neurons.

小剂量藜芦碱可在大鼠脑海马CA1区锥体神经元上诱发出阵发性去极化漂移样癫痫活动。

Recertly, the intrinsic nature of PDS has received more attention.OBJECTIVE: To observe the characteristics of epileptiform activities of rat hippocampal CA1 pyramidal neurons induced by low-dosage veratridine and investigate its possible ion mechanism.

近年来，阵发性去极化漂移的内因机制得到进一步关注和重视。；目的：观察小剂量藜芦碱诱发大鼠脑海马CA1区锥体神经元产生癫痫样活动的特征，探讨其可能的离子机制。

Furthermore, the roles that veratridine plays in the emergence of the parabolic bursting, namely inhibiting the inactivation of sodium channels and eliciting the slow sodium influx, are clarified.

进而阐明了藜芦碱引起这一放电形式所起的作用，即抑制钠通道失活引发慢变钠内流。这种利用非线性动力学理论的分析方法可能会为分析药物的药物动力学提供一种新的途径。

RESULTS: After perfusion of 0.5 μmol/L veratridine, the rat pyramidal neurons in CA1 area displayed relatively fixed-mode of runs of PDS bursting, followed by the hyperpolarization of cell membrane.

小剂量藜芦碱可在大鼠脑海马CA1区锥体神经元上诱发出阵发性去极化漂移样癫痫活动。

BmK IT2 associateed with and dissociated from its binding sites on insect synaptosomes quickly in two phase kinetic manner, and its specific binding was independence of membrane potential, but could be significantly inhibited by native BmK IT2, BmK AS and BmK AS-1 in a dose-dependent manner, and only partially by BmK Ⅰ or veratridine at high concentration.

BmK IT2能以两相动力学方式快速与昆虫突触体结合和解离，且其结合不依赖于膜电位，可分别被天然毒素BmK IT2，BmK AS和BmK AS-1剂量依赖地抑制，另也可被高浓度BmK Ⅰ和藜芦碱部分抑制。

Methods By means of whole-cell patch clamp technique, the epileptiform discharge model of rats hippocampal CA1 neurons was constructed with extracellular perfusion of 0.5 μmol/L veratridine, and the model should be regarded as successfully estabilshed if bursting discharge emerged within 30 min perfusion.

通过膜片钳全细胞记录技术，胞外灌流0.5μmol/L藜芦碱，制作大鼠海马CA1锥体神经元癫痫样放电模型，如果在灌流30min内出现振荡样放电，即认为造模成功。

Radiolabeled pharmacological binding assay showed: 1 BmK AS bound specifically to a single class of non-interacting binding sites on both rat brain and cockroach nerve cord synaptosomes with high affinity and low low capacity. The association of BmK AS with both synaptosomes was quick to reach equilibrium, but the dissociation seemed to be only partial. The specific binding of BmK AS was independent of membrane potential, and couldn't be modified by veratridine. Moreover, the binding of BmK AS could be inhibited by native BmK AS, BmK AS1, BmK IT2 and BmK IT on both synaptosomes, but not or partially inhibited by BmK Ⅰ and suggesting BmK AS type neurotoxins might bind to a distinct receptor site on mammal and insect sodium channels with a similar manner from β-scorpion toxins.

二。蝎毒素多肽配体与哺乳动物和昆虫靶离子通道受体的药理结合特性及其靶结合位点的研究：经放射性标记结合和竞争抑制实验表明：1）BmK AS在大鼠脑和蟑螂神经索突触体上有单一非协同性高亲和力低结合容量结合位点，能快速结合并达到平衡，但仅能部分解离，其结合不依赖于膜电位，也不受藜芦碱调节，天然的BmK AS，BmK AS-1，BmK IT2和BmK IT可抑制〓AS与两种突触体的特异结合，而BmK Ⅰ无或仅有部分抑制效应。

Objective To observe the effects of low dose of veratrine on the discharges of rat hippocampus pyramidal neurons, and to elucidate its possible cytological mechanism.

目的 观察小剂量藜芦碱对大鼠海马CA1锥体神经元放电的影响，并对其诱发产生癫痫样放电的离子机制进行初步探讨。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新