藜芦

Furthermore, the roles that veratridine plays in the emergence of the parabolic bursting, namely inhibiting the inactivation of sodium channels and eliciting the slow sodium influx, are clarified.

进而阐明了藜芦碱引起这一放电形式所起的作用，即抑制钠通道失活引发慢变钠内流。这种利用非线性动力学理论的分析方法可能会为分析药物的药物动力学提供一种新的途径。

RESULTS: After perfusion of 0.5 μmol/L veratridine, the rat pyramidal neurons in CA1 area displayed relatively fixed-mode of runs of PDS bursting, followed by the hyperpolarization of cell membrane.

小剂量藜芦碱可在大鼠脑海马CA1区锥体神经元上诱发出阵发性去极化漂移样癫痫活动。

BmK IT2 associateed with and dissociated from its binding sites on insect synaptosomes quickly in two phase kinetic manner, and its specific binding was independence of membrane potential, but could be significantly inhibited by native BmK IT2, BmK AS and BmK AS-1 in a dose-dependent manner, and only partially by BmK Ⅰ or veratridine at high concentration.

BmK IT2能以两相动力学方式快速与昆虫突触体结合和解离，且其结合不依赖于膜电位，可分别被天然毒素BmK IT2，BmK AS和BmK AS-1剂量依赖地抑制，另也可被高浓度BmK Ⅰ和藜芦碱部分抑制。

In the same neuron, veratridine (1.5～5.0 μmol/L) caused slow wave oscillations of interspike intervals, while aconitine (10～200 μmol/L) caused tonic firing.

结果发现，在同一神经元，藜芦碱(1.5～5.0 μmol/L)可以引起放电峰峰间期的慢波振荡，即峰峰间期由大逐渐减小，然后又逐渐增大，形成重复的振荡波形，每个振荡持续约数十秒至数分钟；而乌头碱(10～200 μmol/L)则引起强直性放电，即峰峰间期逐渐减小，然后维持在一个稳定的水平。

Methods By means of whole-cell patch clamp technique, the epileptiform discharge model of rats hippocampal CA1 neurons was constructed with extracellular perfusion of 0.5 μmol/L veratridine, and the model should be regarded as successfully estabilshed if bursting discharge emerged within 30 min perfusion.

通过膜片钳全细胞记录技术，胞外灌流0.5μmol/L藜芦碱，制作大鼠海马CA1锥体神经元癫痫样放电模型，如果在灌流30min内出现振荡样放电，即认为造模成功。

As noted above,membrane-stabilizers unexpectedly improved the heart function andprevented ER stress both in vivo and in cultured cells.We know that the membranestability depends on the sodium homeostasis in fact.To investigate whether ER stress isassociated with sodium channel action,which determins the membrane stability,wepretreated H9c2(2-1) cells with veratridine,a sodium channel activator.

上面的研究发现膜稳定剂在体内体外均抑制了内质网应激，并改善了心脏功能，而膜稳定作用实际上是维持细胞钠离子稳态的结果，为了研究内质网应激反应与钠离子活动的关系，我们用一种钠通道激活剂藜芦定刺激H9c2(2-1)细胞，30分钟时GRP78表达增加；24小时时PERK磷酸化和CHOP蛋白表达显著增加，说明钠离子通道过度激活诱发了内质网应激反应。

Radiolabeled pharmacological binding assay showed: 1 BmK AS bound specifically to a single class of non-interacting binding sites on both rat brain and cockroach nerve cord synaptosomes with high affinity and low low capacity. The association of BmK AS with both synaptosomes was quick to reach equilibrium, but the dissociation seemed to be only partial. The specific binding of BmK AS was independent of membrane potential, and couldn't be modified by veratridine. Moreover, the binding of BmK AS could be inhibited by native BmK AS, BmK AS1, BmK IT2 and BmK IT on both synaptosomes, but not or partially inhibited by BmK Ⅰ and suggesting BmK AS type neurotoxins might bind to a distinct receptor site on mammal and insect sodium channels with a similar manner from β-scorpion toxins.

二。蝎毒素多肽配体与哺乳动物和昆虫靶离子通道受体的药理结合特性及其靶结合位点的研究：经放射性标记结合和竞争抑制实验表明：1）BmK AS在大鼠脑和蟑螂神经索突触体上有单一非协同性高亲和力低结合容量结合位点，能快速结合并达到平衡，但仅能部分解离，其结合不依赖于膜电位，也不受藜芦碱调节，天然的BmK AS，BmK AS-1，BmK IT2和BmK IT可抑制〓AS与两种突触体的特异结合，而BmK Ⅰ无或仅有部分抑制效应。

Objective To observe the effects of low dose of veratrine on the discharges of rat hippocampus pyramidal neurons, and to elucidate its possible cytological mechanism.

目的 观察小剂量藜芦碱对大鼠海马CA1锥体神经元放电的影响，并对其诱发产生癫痫样放电的离子机制进行初步探讨。

Veratrine has anticarcinogenic chemical activity in the key stages of the development of cancer cells. It can kill the cancer cells and has no harm to the healthy cells.

白藜芦醇在癌细胞的起始，促进，发展三个重要阶段均有化学防癌的活性，能杀死癌细胞而对正常的细胞无毒害作用。

Methods : Using direct pressure carotid tail artery and indirect pressure , duodenum and gavage to Veratrine . Determination of its normal cats , blood pressure in hypertensive rats and the impast of rapic tolerance .

采用颈动脉直接测压法和尾动脉间接测压法，十二指肠和灌胃给藜芦生物碱，测定了其对正常猫，高血压大鼠血压的影响及其快速耐受性。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。