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Thischaracteristic of Atriplex canescens is consistent with that of many local species. TheLCP of Atriplex canescens in different treatments (light, medium, no and heavy watersoil stress) are 314.8μmolm~(-2)s~(-1)、272.5μmolm~(-2)s~(-1)、176.7μmolm~(-2)s~(-1)、143.1μmolm~(-2)s~(-1), respectively, which indicates that the LCP of Atriplex canescens is reducingwith the escalation of soil water stress. This tendency is favorable for Atriplexcanescens under drought stress to maintain certain photosynthesis even in weaksunlight so as to ensure the survival of itself, and keep balance between maintainingwater content inside and its photosynthesis.

四翅滨在不同土壤水分胁迫下的光补偿点:轻度土壤水分胁迫、中度土壤水分胁迫、无土壤水分胁迫和重度土壤水分胁迫下的光补偿点分别为,314.8μmolm~(-2)s~(-1)、272.5μmolm~(-2)s~(-1)、176.7μmolm~(-2)s~(-1)、143.1μmolm~(-2)s~(-1),说明四翅滨的LCP随着土壤水分胁迫的加重而呈现降低的趋势,这种变化有利于处在干旱胁迫中的四翅滨在光强较弱的条件下仍然能维持一定的光合作用,以满足植物自身生命活动所需,同时也可以使植物在保持体内水分和进行光合作用之间寻找平衡。

The colony forming and the cell cycle were analyzed with flow cytometry, and the mRNA expression of bcl-2, c-myc gene were determined with RT-PCR method. Results Resveratrol could markedly inhibit proliferation of human gastric cancer cells compared with that of the blank control group. The cell cycle was blocked to G0/G1. The mRNA level of bcl-2 and c-myc gene were decreased compared with that of the blank control group.

结果 白芦醇可抑制胃癌细胞增殖和集落形成能力,低、高剂量组的抑制率分别为6.9%,17.6%和27.98%,63.39%,差异均有统计学意义(P.05);白芦醇能使胃癌细胞周期阻滞于G0/G1期,明显下调胃癌细胞bcl-2、c-myc基因mRNA表达水平(P.05);用药24 h后,白芦醇高剂量组bcl-2和c-myc基因相对表达量分别为(0.54±0.04),(0.58±0.05),与空白对照组(0.91±0.05),(0.85±0.03)比较,差异均有统计学意义(P.05)。

The expression level of metabolitic enzyme (CYP1A1, CYP1B1) and its correlation with cell differentiation /apoptosis were studied as well. The influence of resveratrol in cell cycle was determined with FCM. Results 1 Resveratrol not only suppresses the growth of medulloblastoma cells in a dose and time related fashion but also induces cell differentiation and apoptosis. Resveratrol promotes differention of UW228-1,-2 cells to forward glia, UW228-3 and Med-3 cells to neuron. A treatment of resveratrol in the concentration of 100μM for 48hrs comit most of cells die of apoptosis. 2 Among the four cell lines Fas and Caspase-3 were constantly expessed, whereas FasL was absent irrespective to the drug treatment. 3 ICC, RT-PCR, Western-blot hybridization and EROD enzymatic activity assay demonstrated that expression of CYP1A1 is different after treatment with resveratrol in four cell lines, up-regulated in three UW228 cell lines in a dose dependent manner but down-regulated in Med-3 cells. Suppressive effect of resveratrol on CYP1B1 expression is the same among four medulloblatoma cell lines. 4 Cell cycle distribution of UW228-3 was greatly changed after treatment.

结果:1、白芦醇以时间和剂量依赖性方式抑制髓母细胞瘤细胞生长,同时促进细胞的分化和凋亡:白芦醇促使UW228-1、-2靶细胞向神经胶质细胞方向成熟分化,UW228-3、Med-3细胞向神经元细胞方向成熟分化,以100μM作用48小时效果显著;2、四个细胞系均有Fas和Caspase-3表达,但无Fas-L基因表达和蛋白活性产生,因此难以形成Fas相关性死亡通路;3、白芦醇处理前后CYP1A1基因的表达在各细胞系略有不同:UW228-1,-2,-3三个细胞系白芦醇以剂量相关性方式上调CYP1A1的表达,而Med-3细胞系,白芦醇却抑制CYP1A1的表达;四个细胞系在白芦醇处理后CYP1B1的表达均受到抑制;4、UW228-3细胞系经白芦醇作用后细胞周期有较大改变,表现在G0/G1期在整个细胞周期中所占的比例增加,而其它时期则相应减少。

The results indicated that resveratrol content in different producing areas varied during the fermentation and it increased gradually along with the fermentation and the addition of pectase and it reached the highest level up to chief fermentation, then it decreased and maintained a certain level till malolactic fermentation.

结果表明,尽管不同产地在发酵过程中生成的白芦醇含量有所不同,但都随着果胶酶添加和酵母发酵的进行,白芦总醇含量逐渐上升,至主发酵时白芦总醇含量达到最高,进入苹果酸-乳酸发酵后,白芦醇含量有所下降并维持一定的水平。

ReMV causes necrotic lesions on hosts such as Nicotiana glutinosa, Nicotiana rustica, Datura stramonium, Chenopodium quinoa, Chenopodium amaranticolor and Tetragonia expansa and causes systemic mosaic on hosts such as Nicotiana tabacum L, Nicotiana tobacum CV. K326, Lycoporsicon .esculentum and Brassica pekinensis.

对ReMV在寄主植物上的症状表现进行了测定,结果表明,ReMV可以侵染茄科、番杏科、十字花科和科等4科10种植物,其中在茄科的心叶烟、黄花烟、蔓陀罗;番杏科的番杏;科的苋色和昆诺上表现为局部枯斑症状。

Amino-6-methoxy Benzothiazole 99%、2-Amino Benzothiazole 99%、Veratraldehyde 99%、Veratric Acid 99%、1,2- Dimethoxy Benzene 99%、6-Nitroveratric Acid 97%、2,5-Dichlorobenzoic Acid 99%、4-Chloro-3-Nitrobenzoic Acid 99%、 3-Nitrobenzole Acid 98%、p-Methoxybenzoie Acid 99%、Anisole 99.5%、p-Methoxyacetophenonc 99%、(2'-Chloroethyl) Benzene 99%、(2'-Bromoethyl) Benzene 99%、N,N-Diethyi-m-Toiuamide 99%、Aminoacetonitrile Hydrochloride 98%、 Alkyl Diphenyl Phosphate Estrel 98%、5-Chloro-2-benzothiazolone 99%、6-bromo-2-naphthol 98%、6-Nitro-Veretric Acid Estrel 97%、4-chloro phenoxyaeetic 99

2-氨基-6-甲氧基苯骈噻唑、2-氨基苯骈噻唑、芦醛、芦酸、芦醚、6-硝基芦酸、2,5-二氯苯甲酸、3-硝基-4-氯苯甲酸、3-硝基苯甲酸、对甲氧基苯甲酸、苯甲醚、对甲氧基苯乙酮、β-氯代苯乙烷、β-溴代苯乙烷、N,N-二乙基间甲苯甲酰胺、氨基乙腈盐酸盐、磷酸二苯月桂酯、5-氯-2-苯骈噻唑酮、6-溴-2-萘酚、6-硝基芦酸甲酯、4-氯苯氧乙酸

To establish a MPLC method for the content determination of resveratrol in Polygonum cuspidatum Sieb. Et. Zucc, Analytical column SOURCE 5RPC ST 4.6/150 was used with a mixture of acetonitrile+water (30+70,volume ratio ) as mobile phase ,with a flow rate of 1ml/min and the UV detection wavelength was 306nm, The linear range was 0.6-1.6mg/ml (r=0.9919). The average recovery of prodatin was 103.0%-90.4%(RSD=7.21%). The method is convenient and accurate. Result indicated a reverse phase chromatography media based o n polystyrene/divinyl benzene has excellent resolution for resveratrol in cuspidatum. P rovide theoretical support for process development.

色谱条件采用SOURCE 5RPC ST 4.6/150预装柱,以乙腈/水(体积比30/70)溶液为流动相,流速1ml/min、检测波长为308nm,结果表明,白芦醇在0.6~1.6mg/ml的浓度范围内峰面积积分值与浓度的相关系数r=0.9919;所测定的虎杖饮片中白芦醇平均含量为0.304%,平均回收率为103.0%~90.4%;实验表明,以聚苯乙烯为基架的反相介质对于虎杖中自芦醇具有良好的分辨率,适合其有效成分的检测、分离,对虎杖中白芦醇制备的科学研究过渡到工艺开拓有很好的借鉴。

Oxidation veratryl alcohol by LAC/NHA or HBT was 0 degree reaction, but the coefficient of the reaction with NHA is bigger than that with HBT, which means that NHA is more efficient for LMS delignification than HBT.

此外,还比较了NHA和HBT作为介体的LMS体系氧化非酚型木素模型化合物芦醇的动力学规律,结果表明芦醇的氧化与时间呈直线关系,此氧化反应为0级反应,不过使用NHA和HBT作为介体时,芦醇的氧化速度常数有所不同,使用NHA时,芦醇氧化的速率常数大,反应速度快。

The DRe differences among plants could be well differentiated by TP. The DRe., i.e. water retention, represented by TP, was used as a major index to identify the ability of plant drought tolerance. It was showed that C.arborescens Ecotype Wu was generally the most drought tolerant one except lower than C.arborescens Ecotype XJ which was the maximum in May, bore comparison with Zygophyllum xanthoxylum by estimating seasonally; C.latens Ecotype NX was significantlly lower than C.arborescens Ecotype Wu, the result was contrary to being known; C.arborescens Ecotype Kerqin was significantlly lower than Wu; XJ, the maximum in May, showed no else great difference to NX; C.lanata was high in April and not high afterwards, however its phenological phase presented visible changes as compared with the original life patterns in western America.

结果表明,华北驼绒(C.arborescens Ecotype Wu)总体上表现出最强的耐旱性;宁夏驼绒(C.latens Ecotype NX)耐旱性显著低于华北驼绒;科尔沁驼绒(C.arborescens Ecotype Kerqin)耐旱性显著低于华北驼绒;新疆驼绒(C.arborescens Ecotype XJ)5月耐旱性表现最强,其他时间与宁夏驼绒差别不大;北美驼绒4月拐点很高,4月以后耐旱性降低,其物候期与原分布区相比发生了很大变化。

The resveratrol has pharmacologic action of antioxidant、anti-free radical and effecting the metabolismof arachidonic acid. The method which was used for quantitating the resveratrol of Smilax glabra byRP-HPLC was established,furthermore, the content of resveratrol from different areas were compared, thecontent of resveratrol from hunan xiangtan was the highest.

芦醇具有抗氧化、抗自由基及影响花生四烯酸代谢的药理作用,本实验建立了土茯苓中白芦醇含量的RP-HPLC测定方法,并对不同产地土茯苓中自芦醇含量进行比较,其中湖南湘潭白芦醇含量最高。

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