藏花

The method of solvent together with macroporous resin extracting and separating crocin and geniposide was investigated firstly in China.

国内首次研究了溶剂浸提与大孔树脂相结合的方法对藏花素、栀子甙的提取和分离的影响，结果表明：Ⅰ。

Objective: To investigate the proliferation, apoptosis and mechanisms on T24 cell of transitional cell carcinoma of bladder by crocin.

中文摘要：目的：研究藏花素对膀胱移行细胞癌增殖、凋亡的影响及作用机制。

MTT assay was adopted to determine the inhibition rate for selecting the best effect time and concentration of Crocin.

采用MTT法测定不同浓度及不同时间藏花素对T24细胞抑制率，选出最佳药物浓度和最佳作用时间。

Objective: To investigate the changes of gene expression profile in transitional cell carcinoma of bladder T24 cell after crocin treatment, in order to find the possible crocin targets.

中文摘要：目的：应用基因表达谱芯片研究藏花素对膀胱移行细胞癌T24细胞基因表达谱的影响。

Supercritical fluid carbon dioxide can extract crocin and geniposide, but only under the condition of high temperature, high pressure and adding a great deal of modifier, the extracting rate can be up to above 90％.Ⅱ.

超临界CO〓对藏花素和栀子甙具有一定的萃取能力，但需在高温高压及加大量夹带剂的情况下，萃取率才可达90％以上。Ⅱ。

Objective: To study the effect of Crocin on the expression of VEGF-C in transitional cell carcinoma of bladder T24 cell. Methods: 24 cells were treated with Crocin.

目的：研究藏花素对膀胱移行细胞癌T24细胞血管内皮生长因子C(Vascular endothelial growth factor， VEGF-C)表达水平的影响。

Crocin inhibits the proliferation of T24 cells and the mechanism might be decreasing tumor angiogenesis by down-regulating the expression of VEGF-C.

藏花素抑制T24细胞增殖，可能与下调VEGF-C的表达，从而减少肿瘤血管的生成有关。

Result: The growth of T24 cells was inhibited remarkably following a marked positive correlation between crocin concentration, time and inhibitor rate.

结果：MTT实验结果显示3 mmol·L-1藏花素作用T24细胞48 h的抑制率与对照组相比有显著意义( P 。05)。

RT-PCR showed that VEGF-C mRNA expression in Crocin treated group was significantly lower than that of the control group. The cDNA microarray analysis was coincided well with the RT-PCR data.

RT-PCR显示藏花素组VEGF-C表达明显减少，基因芯片扫描结果中藏花素组VEGF-C表达也显著下调。

Results: The growth of T24 cells was inhibited significantly P.05 at 3mmol/L Crocin when treated for 48h. And there was a marked positive correlation between Crocin concentration, time and inhibition rate.

结果：MTT显示藏花素抑制T24细胞生长具有时间和剂量依从性，3mmol/L藏花素作用48h对T24细胞的抑制率最高。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。