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The plant expression vectors were constructed and two genes were transferred into A. thaliana and N. tabacum, antikanamycin plants were obtained.4. The trials of adventive bud regeneration directly and callus induction was conducted to select out the optimal explants and formula. The results revealed that double scale can produce 13~16 adventive buds per centimeter in MS medium added 10.0 mg/L 6-BA, 1.0 mg/ L NAA, 40 g/L sugar and 7.5 g/L agar. Scale, leaves, scape and different tissue of flower can be induced to produce callus in appropriate medium, but the filament with anther was the optimized explant.

构建了两个基因的pBI121和pCAMBIA1300植物表达载体,并通过农杆菌将上述基因转入烟草和拟南芥中,得到了卡那抗性的再生植株。4、以中国水仙不同部位外植体进行了不定芽直接分化和愈伤组织诱导分化试验,结果表明,双鳞片外植体在MS0+6-BA 10.0 mg/L+NAA 1.0 mg/L+蔗糖40 g/L+琼脂7.5 g/L培养基上可以大量地直接再生,再生芽数量平均可达13~16个/cm双鳞片,比传统的鳞茎盘之间分化提高30~50%;鳞片、叶片、花葶及花部不同组织在适宜的配方中均可不同程度地诱导出愈伤组织,但以带花药的花丝诱导出的愈伤组织最好。

The callus induction best culture medium is WPM ZT 1.5mg/L (above inculture medium sugar is 2.0%, agar-agar is 0.8%, the PH value is 5.6), its induction leads reaches as high as 88.0%.

愈伤组织诱导的最佳培养基为WPM ZT 1.5mg/L(以上培养基中均加入蔗糖2.0%,琼脂0.8%,PH值为5.6),其诱导率高达88.0%。

The rhizosphere soil microorganism and rhizosphere soil enzyme activity werepositive correlated with each other.Except aerobic bacteria and actinomycetes andfungi did not achieve significant or extreme significant correlation with urease,theother factors all achieved significant or extreme significantcorrelation.Besides,through a correlation analysis between rhizosphere agrobiologyand rhizosphere soil nutrient,except rapidly available K was negative correlation with urease and catalase,the other agrobiology factors were positive correlation with soilnutrient, aerobic bacteria and sucrase and catalase were extreme significantcorrelation with alkaline hydrolytic N and organism matter and rapidly available P;actinomycetes was extreme significant correlation with all soil nutrient factors;fungiwas extreme significant correlation with alkaline hydrolytic N and organism matterand rapidly available P, significant correlation with rapidly available K;urease wasextreme significant correlation with alkaline hydrolytic N, significant correlation withrapidly available P.

本试验所测定的根系土壤微生物与根系土壤酶活性间均呈正相关,除了好、气性细菌、放线菌、真菌与脲酶间未达到显著或极显著相关外,其余因子间均呈显著或极显著相关;此外,通过根系土壤生物学因子与根系土壤养分相关分析,发现除了速效K与脲酶、过氧化氢酶呈负相关外,其余生物因子与土壤养分因子间均呈正相关,其中,好气性细菌、蔗糖酶、过氧化氢酶与水解N、有机质和速效P极显著相关;放线菌与所有养分因子间均极显著相关;真菌与水解N、有机质和速效P极显著相关,与速效K显著相关;脲酶与水解N极显著相关,与速效P显著相关。

The stability of aloin decreased with the increase of temperature and heating time. However, the absorbency of aloin did not change with time under room temperature (20℃). The aloin was sensitive to light, and the aloe juice showed a yellowish green sediment in 2 hours after exposed to sunlight. The stability of aloin declined when the pH value arose. The aloe juice was more stable under lower concentration of sodium chloride and sucrose.

结果表明:加热的温度越高,时间越长,芦荟苷的热稳定性越差,而在室温下(20℃)芦荟苷的吸光度基本不随时间改变;芦荟苷具有光敏性,在太阳光照射下,2小时后便出现黄绿色沉淀物;芦荟溶液随着pH的升高,其稳定性下降;低浓度的氯化钠和蔗糖对芦荟汁液稳定性的维持比高浓度的效果明显。

By comparing the activity of theα-amylase and the soluble acid invertase in the kernel and the pedicel, the trend of theα-amylase activity in the kernel and the pedicel showed a peak, theα-amylase activity was high in the early stage, it could promoted amylolysis, supply the nutrition for the kernel development; theα-amylase activity was low in the rapid filling stage, it was in favorable to the starch accumulation.

通过比较籽粒和小穗柄中α-淀粉酶和可溶性酸性蔗糖转化酶的活性表明,籽粒和小穗柄中α-淀粉酶活性均呈单峰变化趋势,籽粒发育早期α-淀粉酶活性较高,可以促使淀粉转化,为籽粒的早期的发育提供营养物质;籽粒发育中期为快速灌浆期,淀粉酶活性较低,利于淀粉的积累。

Intravenous iron sucrose is an effective and safe agent for the treatment of iron deficiency in anemic hemodialysis patients.

蔗糖铁是治疗伴有缺铁的血液透析患者肾性贫血的一种安全而有效的药物。

The optimum making-up prescription of Angeleno and almond compoundprotein beverage is that: the ratio of Angeleno juice/almond slurry is 10:11, mixedemulsifier (span 60/tween 40=25:11) 0.13%, mixed stabilizer (xanthan gum/carrageenan=1:2) 0.12%, sugar 10%.

安哥诺李、杏仁复合蛋白饮料最佳配方:安哥诺李汁和杏仁浆的配比为10:11,复合乳化剂(HLB=8)(司盘60:吐温40=25:11)为0.13%,复合稳定剂(黄原胶:卡拉胶=1:2)为0.12%,蔗糖为10%。

Other Ingredients: Glucose Syrup, Sucrose, Gelatin, Citric Acid, Lactic Acid, Colors (Black Carrot Juice Concentrate, Annatto and Turmeric), Natural Flavors, Fractionated Coconut Oil and Beeswax to Prevent Sticking.

其他成分:葡萄糖浆,蔗糖,明胶,柠檬酸,乳酸,颜色(黑色胡萝卜浓缩汁,胭脂树和姜黄),天然香料,分次椰子油和蜂蜡防止坚持。

Other Ingredients: Glucose Syrup, Sucrose, Gelatin, Citric Acid, Lactic Acid, Colors (Annatto, Black Carrot Juice Concentrate and Turmeric), Natural Flavors, Fractionated Coconut Oil and Beeswax to Prevent Sticking.

其他成分:葡萄糖浆,蔗糖,明胶,柠檬酸,乳酸,颜色(胭脂树,黑胡萝卜浓缩汁和姜黄),天然香料,分次椰子油和蜂蜡防止坚持。

Glucose Syrup, Sucrose, Gelatin, Fractionated Coconut Oil, Natural Flavors, Citric Acid, Colors (annatto, black carrot and turmeric), Lactic Acid and Beeswax.

葡萄糖,蔗糖,凝胶,椰子油,天然口味,柠檬酸,天然颜色(胭脂树,黑胡萝卜和姜黄),乳酸,蜂蜡。

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