蔗糖

The results showed that: different concentrations of sucrose and boric acid had significant impact on pollen germination, but different concentrations of calcium had no significant impact on pollen germination. It was most appropriate for tube elongation when sugar 150 gL^(-1) and boric acid 100 gL^(-1). Lack of sucrose would lead to the accumulation of callose in top of pollen tube and hold back its growth. The distribution of callose was regular in pollen tube when the medium lacked boric.

结果表明：蔗糖、硼酸浓度间对花粉萌发有显著影响，钙浓度间对花粉萌发的促进作用不明显；蔗糖150 gL^(-1)、硼酸100 mgL^(-1)时最适宜花粉管伸长；蔗糖的缺乏会导致胼胝质在花粉管顶端积累，使其生长停滞，缺硼时胼胝质在花粉管内分布正常。

Insecticidal sucrose octanoate and sucrose caproate were synthesized by transesterification method or reaction of acid chloride with sucrose.

分别以酯交换法和酰氯法合成了辛酸蔗糖酯和己酸蔗糖酯两种杀虫活性蔗糖酯，其中酯交换法产物单酯含量高，而酰氯法反应速度快。

Firstly,saponifying of caustic potash and fatty acid ether esters to convert partial neutral fatty acid soap to emulsifier,and adding sucrose together as emulsifier,making sucrose and fatty acid ether esters come to moloten state at lower temperature,further making interesterification turns out sucrose fatty acid esters at homogeneous phase .

首先使苛性钾与脂肪酸乙酯反应生成部分中性皂作为乳化剂，并加入一定量的蔗糖，使蔗糖和脂肪酸乙酯在较低温度下既可达到相溶状态，进而在均相下发生酯交换反应合成蔗糖酯。

It was seen that strain 6055 generated three kinds of dextransucrases whose molecular weights were 151 kDa,142kDa and 117kDa,respectively.Among them,the former two ones were found both in supematants and cell wall,while the later was only found in the fermentation liquor.Strain DM 1-2 synthesized two kinds of dextransucrases,and their molecular weights were 183kDa and 142kDa,respectively.The former existed both in cell wall and out of cell,but the later only was located on cell surface.Strain PC 13 produced three kinds of dextransucrases,and their molecular weights were 148kDa, 138kDa and 115kDa,respectively.The former two ones were ectoenzyme while the later was linked to cell.Resembled to strain 6055,strain L4 produced two types of dextransucrases coexisting in supernatants and cell wall with molecular weights of 145 kDa and 136kDa,respectively,but the dextransucrase of 115kDa was only found in free-cells supernatants.

结果显示，菌株6055可产生分子量分别为151kDa、142kDa和117kDa的三种葡聚糖蔗糖酶，其中前两者同时存在于上清液中和细胞表面，而后者只存在于上清液当中；菌株DM1-2可以产生两种葡聚糖蔗糖酶，分子量分别为183kDa和142kDa，前者同时存在于上清液中和细胞表面，而后者仅能存在于细胞表面；菌株PC13可以产生分子量分别为148 kDa、138 kDa和115kDa的三种葡聚糖蔗糖酶，前两者属于胞外酶，而后者与细胞相连；与菌株6055相似，菌株L4可以产生两种同时存在于上清液和细胞表面的葡聚糖蔗糖酶，分子量分别为145 kDa和136kDa，而分子量为115kDa的葡聚糖蔗糖酶只存在于上清液中。

The strawberry fruit were sampled from 1 to 8 weeks after full blossom. The contents of sucrose, glucose and fructose and the activities of sucrose phosphate synthase, sucrose synthase, acid invertase, neutral invertase, hexokinase and fructokinase were measured in developing strawberry fruits.

采集花后1~8周的果实，分别测定果实中葡萄糖、果糖、蔗糖的含量和蔗糖磷酸合酶、蔗糖合酶、可溶性酸性转化酶、中性转化酶、己糖激酶及果糖激酶等蔗糖和己糖代谢酶的活性。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Fruits of mango cultivar Yuexi No.1(Mangifera indica L.) at the mature stage were collected and detected in terms of starch, glucose, fructose and sucrose, as well as acid invertase, neutral invertase, sucrose synthase and sucrose phosphate synthase.

以粤西1号为试材研究了芒果果实成熟阶段淀粉、葡萄糖、果糖和蔗糖的含量以及蔗糖代谢相关酶-酸性转化酶、中性转化酶、蔗糖合成酶和蔗糖磷酸合成酶的活性变化。

SUC2 mainly encodes sucrose invertase. The invertase could hydrolyse sucrose into glucose and fructose which are carbon source for yeast growth. When sucrose is used as the main carbon source, invertase is necessary for the growth of yeast. Accordingly, it is of great significance for yeast glycometabolism to study the sequence, transcription and regulation of sucrose invertase.

蔗糖转换酶基因(SUC2)编码蔗糖转换酶，通过降解酵母细胞外的蔗糖成果糖和葡萄糖来提供酵母生长所需的碳源，在以蔗糖为主要碳源的培养条件下，蔗糖转换酶对于酵母生长必不可少，因此研究蔗糖转换酶基因序列、转录、调控等对酵母蔗糖代谢具有重要意义。

The research on the tissue culture and cell suspension culture showed that thesuitable culture medium for inducing bud was MS supplemented with 1.5mg/L 6-BAand 0.1mg/L NAA, and for the generation-continuing multiplication was MSsupplemented with 1.5mg/L 6-BA and 0.2mg/L NAA. The rooting medium was1/2MS supplemented with 0.5mg/L IBA and the rooting rate was 45.0%. Plantletsurvival after transfer to sand was 52.5%.The induction rate of calli was66.7%～86.6% and the optimum medium was MS medium with 0.5mg/L 6-BA and2.0mg/L 2,4-D. The calli became smallest partical size, friable and had gooddispersion ability after 3 times successive transfer culture, the optimum medium wasMS medium with 0.2mg/L 6-BA and 2.0mg/L 2,4-D. Culturing these particles on sixkinds of MS liquid media with different hormone contents, the optimum medium wasselected basing on he change of the density of single-cell, the density of cellaggrefate and the mass of cell.

对蒜头果进行的组织培养与细胞悬浮培养研究结果表明：MS+6-BA1.5mg／L+NAA0.1mg／L+蔗糖3％激素组合能够较好地诱导芽的初始分化和增殖，适宜的芽苗继代增殖培养基为MS+6-BA1.5mg／L+NAA0.2mg／L+蔗糖3％；采用1／2MS+IBA0.5mg／L+蔗糖3％为生根培养基，生根率为45.0％；移栽到河沙的生根苗成活率为52.5％；愈伤组织诱导率为66.7％～86.6％，其中以MS+6-BA0.5mg／L+2，4-D2.0mg／L+蔗糖3％的培养基最佳，其诱导出的愈伤组织具有较强的增殖能力和较好的脆散结构，最佳继代培养基为MS+6-BA0.2mg／L+2，4-D2.0mg／L+蔗糖3％，且培养基中的6-BA与2，4-D浓度的比值越小，愈伤组织生长越快，结构越脆散，增殖率越高；将继代后的愈伤组织转入6种含不同激素浓度组合的MS液体培养基中进行振荡培养，在综合分析各培养基的单细胞密度，细胞团块密度，细胞生物量增长率等指标后，初步筛选出MS+6-BA0.2mg／L+2，4-D2.0mg／L培养基为较好的液体培养基。

Compared with the ck, the sugar accumulation increased extremely significant by grafting with the wild watermelon 2, but that of the other treatments all decreased and the sugar accumulation of loofah/Zaojia was the lowest. The sucrose invertase activity decreased; the activitives of sucrose phosphate synthase and net for sucrose-metabolism enzymes all increased during the development of own-rooted and grafted watermelons.

与对照相比，野生西瓜2号／早佳组合的果实糖积累极显著增高，而其他嫁接组合的糖积累均降低，丝瓜／早佳组合的果实糖含量最低；自根和嫁接西瓜果实发育过程中蔗糖转化酶活性降低，蔗糖磷酸合酶活性升高，蔗糖合酶在西瓜果实发育后期主要起合成作用，促进果实蔗糖积累，蔗糖代谢相关酶净活性值增大。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。