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Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖,琼脂0.7%,pH5.8 培养基中25℃暗培养20d 后继代一次,诱导产生Ⅱ型胚性愈伤组织,用镊子将其夹碎成2mm2大小的小块,置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中,侵染时间为45min,共培养3 天后,转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天,再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中,遮光培养20d后,置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化,卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%;同时以甘蔗心叶愈伤组织材料,通过循环水式真空泵,产生负压,设定不同的负压值,在农杆菌菌液中感染5min 后,在负压条件下继续侵染15min 后,筛选出抗性愈伤组织并获得转化植株,其中在负压为-0.05 时转化率达到最大值,其卡那霉素抗性愈伤组织获得率平均为37.5%。

Systematic isopiestic determinations have been made at the temperature 298.15 K for the mixed nonelectrolyte aqueous solution saturated with an electrolyte and a nonelectrolyte {water (A1)+sodium chloride〓(A2)+glycine〓(A3)+sorbitol+ sucrose}, the mixed nonelectrolyte solution saturated with two electrolytes {water (A1)+sodium chloride〓(A2)+barium chloride〓(A3)+sorbitol +sucrose}, and the mixed electrolyte solution saturated with another electrolyte and a nonelectrolyte {water (A1)+barium chloride〓(A2)+mannitol〓(A3)+ sodium chloride +ammonium chloride}, taking sodium chloride or calcium as the reference standard.

以NaCl或CaCl〓为参考物,系统地测定了298.15K时一种电解质和一种非电解质饱和的混合非电解质溶液{H〓O(A1)+NaCl〓(A2)+甘氨酸〓(A3)+山梨醇+蔗糖}、两种电解质饱和的混合非电解质溶液{H〓O(A1)+NaCl〓(A2)+BaCl〓(A3)+山梨醇+蔗糖}以及一种电解质和一种非电解质饱和的混合电解质溶液{H〓O(A1)+BaCl〓(A2)+甘露醇〓(A3)+NaCl+NH〓Cl}的等压平衡。

Results showed that B3(IBA+NAA, 300mg/L+300mg/L) was the best treatment in quantity and length of new roots, rate of radication and survival, distributing of roots length; Treatment B4(IBA+NAA, 500mg/L+500mg/L) took the second place; The following treatments in turn was D3 (NAA+VB1+cane sugar, 100mg/L+80mg/L+2%), D4(NAA+VB1+cane sugar, 300mg/L+80mg/L+2%), B2(IBA+NAA, 100mg/L+100mg/L), B1(IBA+NAA, 50mg/L+50mg/L).

结果表明:在生根数量,生根长度,根长分布及成活率和生根率上以处理B3(IBA+NAA,300mg/L+300mg/L)最好,其次是处理B4(IBA+NAA,500mg/L+500mg/L)。然后是处理D3(萘乙酸+维生素B1+蔗糖,100mg/L+80mg/L+2%)、D4(萘乙酸+维生素B1+蔗糖,300mg/L+80mg/L+2%)、B2(IBA+NAA,100mg/L+100mg/L)、B1(IBA+NAA,50mg/L+50mg/L)。

This paper reviews the development of fresh cut flowers industry, development trends and the prospect of fresh cut flowers on a different preservative on Fire Lily of fresh cut flowers effect, results showed that: the preservation of all the bottles to extend Lily Cut Into life, increase the squid fresh weight, increasing Huajing, spent long cut to improve the water situation in which to 2 percent sucrose +200 mg/L8-HQC +200 mg / LSTS +225 mg / L resveratrol deal with the best formula , Followed by two percent sucrose +200 mg/L8-HQC +200 mg / LSTS +500 mg/LAL2 (SO4) 3 +115 mg / L SNP formula.

本文综述了鲜切花产业的发展现状、发展趋势以及切花保鲜的前景,研究了不同保鲜剂对火百合切花的保鲜效应,结果表明:各保鲜剂均能延长百合切花的瓶插寿命,增加花枝鲜重,增大花径、花长;改善切花的水分状况;其中以2%蔗糖+200mg/L8-HQC+200mg/LSTS+225mg/L白藜芦醇配方处理效果最佳,其次是2%蔗糖+200 mg/L8-HQC+200mg/LSTS+500mg/LAL2(SO4)3+115mg/L硝普钠配方。

Different preservative on the fresh cut flower lily effect Abstract: This paper reviews the development of fresh cut flowers industry, development trends and the prospect of fresh cut flowers on a different preservative on Fire Lily of fresh cut flowers effect, results showed that: the preservation of all the bottles to extend Lily Cut Into life, increase the squid fresh weight, increasing Huajing, spent long cut to improve the water situation in which to 2 percent sucrose +200 mg/L8-HQC +200 mg / LSTS +225 mg / L resveratrol deal with the best formula , Followed by two percent sucrose +200 mg/L8-HQC +200 mg / LSTS +500 mg/LAL2 (SO4) 3 +115 mg / L SNP formula.

不同保鲜剂对百合切花的保鲜效果摘要:本文综述了鲜切花产业的发展现状、发展趋势以及切花保鲜的前景,研究了不同保鲜剂对火百合切花的保鲜效应,结果表明:各保鲜剂均能延长百合切花的瓶插寿命,增加花枝鲜重,增大花径、花长;改善切花的水分状况;其中以2%蔗糖+200mg/L8-HQC+200mg/LSTS+225mg/L白藜芦醇配方处理效果最佳,其次是2%蔗糖+200 mg/L8-HQC+200mg/LSTS+500mg/LAL2(SO4)3+115mg/L硝普钠配方。

Adding the threonine has significant impact on the results of acetaldehyde in all of LAB.

Mn2+、蔗糖、Vc的添加对乳杆菌生成乙醛有明显的促进作用。

Other Ingredients: Glucose Syrup, Sucrose, Gelatin, Citric Acid, Lactic Acid, Colors (Black Carrot Juice Concentrate, Annatto and Turmeric), Natural Flavors, Fractionated Coconut Oil and Beeswax to Prevent Sticking.

其他成分:葡萄糖浆,蔗糖,明胶,柠檬酸,乳酸,颜色(黑色胡萝卜浓缩汁,胭脂树和姜黄),天然香料,分次椰子油和蜂蜡防止坚持。

Other Ingredients: Glucose Syrup, Sucrose, Gelatin, Citric Acid, Lactic Acid, Colors (Annatto, Black Carrot Juice Concentrate and Turmeric), Natural Flavors, Fractionated Coconut Oil and Beeswax to Prevent Sticking.

其他成分:葡萄糖浆,蔗糖,明胶,柠檬酸,乳酸,颜色(胭脂树,黑胡萝卜浓缩汁和姜黄),天然香料,分次椰子油和蜂蜡防止坚持。

Glucose Syrup, Sucrose, Gelatin, Fractionated Coconut Oil, Natural Flavors, Citric Acid, Colors (annatto, black carrot and turmeric), Lactic Acid and Beeswax.

葡萄糖,蔗糖,凝胶,椰子油,天然口味,柠檬酸,天然颜色(胭脂树,黑胡萝卜和姜黄),乳酸,蜂蜡。

Sucrose, Glucose Syrup, Apple Pectin, Fumaric Acid, Natural Flavors and Colors (annatto, black carrot juice concentrate, berry juice concentrate and tumeric). L'il Critters Groovy Calcium Gummy Bears are sprinkled with fumaric acid, citric acid and sucrose crystals for a delicious sour flavor.

蔗糖,葡萄糖糖浆,苹果果胶,富马酸,天然风味和颜色(红木,黑色浓缩胡萝卜汁,浆果浓缩汁等) L'il 小熊钙片表面撒满反丁烯二酸类的酸,柠檬酸和蔗糖晶体,因此具有美妙的酸甜口味。

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Mom and Dad better bone up on these not so ordinary competitions like polo, yachting and synchronized swimming!

爸爸妈妈更好的骨落实这些不是普通的比赛一样,水球,帆船和花样游泳!