蒸气的

There were equally excellent opportunities for vacationists in the home island, delightful sylvan spots for rejuvenation, offering a plethora of attractions as well as a bracing tonic for the system in and around Dublin and its picturesque environs even, Poulaphouca to which there was a steamtram, but also farther away from the madding crowd in Wicklow, rightly termed the garden of Ireland, an ideal neighbourhood for elderly wheelmen so long as it didn't come down, and in the wilds of Donegal where if report spoke true the coup d'oeil was exceedingly grand though the lastnamed locality was not easily getatable so that the influx of visitors was not as yet all that it might be considering the signal benefits to be derived from it while Howth with its historic associations and otherwise, Silken Thomas, Grace O'Malley, George IV, rhododendrons several hundred feet above sealevel was a favourite haunt with all sorts and conditions of men especially in the spring when young men's fancy, though it had its own toll of deaths by falling off the cliffs by design or accidentally, usually, by the way, on their left leg, it being only about three quarters of an hour's run from the pillar.

在故乡的岛屿度假的人们也有同样的良机。这里有令人赏心悦目、有助于恢复青春的森林地带，都柏林市内外以及风光绮丽的近郊，不仅富于无上魅力，而且还能促进身体健康。有一条蒸气火车铁轨一直铺设到噗啦呋咔瀑布。还有威克洛那越发远离尘嚣、对"爱尔兰庭园"这一称谓当之无愧的所在。只要不下雨，那一带是供年长的人们骑自行车的理想田园，再有就是多尼戈尔的荒野，倘若传闻属实，景色也极为壮观。不过，由于最后提到的这一地区交通不便，尽管此行可获益匪浅，前往的游客毕竟有限，收入也微不足道。相形之下，霍斯山凭借绢骑士托马斯、格蕾斯。奥马利和乔治四世留下的遗迹，以及遍布于海拔数百英尺高处的杜鹃花，使它成为男女老少不分贫富，人人爱去的地方。由纳尔逊纪念柱乘车前往，只消三刻钟就可到达。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

TyPe II collagen induced arthritisln the rat ank1e joint andoVathumin as antigen induced arthritis WA in the rabbit knee joint wereestab1ish2 Qualitative evaluation of me in skin, muscle, synovium, cedilagearound joint and blood was performed by OMA3 The CIA rats were treated on day 7 after hind paw swelling and erythemaAnimals were injected intravenously with ase at a dose of 10mg/kg,tWenty minuots 1ater, one ankle of the rats random1y assigned was exPosedlaser irradiation at l00J/cm fOr l000 seconds, and another ankle wasM grouP wihout laser The other two groups is unmanipulatedcontrol group and untreated CIA group Bimaleolar ankle widthmeasuremellts were taken in all animals every tWo days using amicrometer The histopathology of the ank1e Joint was assessed at day 21after disease onset4 The pro1iferating cell nuclear antigen WCNA of CIA treated by PDT andthe HMME group without laser was doterdrined by immunohistochemiStry5 The AfA rabbits were treated on day 7 after knee swelling and erythemaThe theraPy invo1ved lntravenous injection of l0mg/kg HMME, fOl1owedby 20 minues period in dim light, and transdermal light treatment with\l00 J/cm2 fOr l000 seconds The inner sides of the treated Anees wereirradiated at first, and then the outer side did 24 hours later, the synovialtissue of the Anees joint were removed and in situ cel1 aPoptosis wasdetCCted With tednal deoxync1eotidyl transferase-mediated dUTP nickend labelingR6suIt8:l The pathologic changes of CIA and AIA include subsynovial inflammation,opovial hyPerplasia, pannus formation, cartilage and bone destructionresemble RA.2 The studies demonstrated that there are different uptake of HMME withinskin, muscle, synovium, cartilage and b1ood, and the synovium cou1draPidly uPtake more ase than skin and cartilage at the firSt 30 minuesaller intravenous injection of HMME3 The bimaleolar anke width had no different among PDT treated group,H group withollt 1aser and untreated CIA group But hlstologicalevaluation showed statiStical1y significallt reductions in synovialhyperplasia, pannus formation and cart1lage reosion, bone destruction andtotal score in PDT treated group4 Image analysis showed that the ratlo bforeen the areas of the coufltedobect to that of the entire area in PDTtreated grOup is lower than that in conirol group, but the integrated oPticaldensity had no different between the two groups5 Imape analysis showed that the ratio between the area of the countedobject to that of the e

治疗组在大鼠出现踝关节红肿后1周，炎症达到高峰时进行PDT治疗。随机治疗大鼠一侧的踝关节，另。2。一一侧作单纯HMME 对照。治疗方法是大鼠麻醉后尾静脉注入 HMME10ngkg，20分钟后踝关节照光，激光波长627.sum，功率密度 100mwcm'，照射时间1000秒，能量密度100）／。治疗后避光喂养72 小时。隔日一次测量大鼠的踝关节左右横径，治疗后两周取关节进行病理d 观察。 4。大鼠CIA模型用上述方法进行PDT治疗后，治疗组和单纯HMME 组用兔疫组化SP法检测石蜡切片的核增殖抗原。 5。兔AIA模型在关节炎出现第七天进行PDT治疗，随机治疗一侧膝关节，另一侧作自身对照。兔耳静脉注入I'arrainrelomg／Kg，20分钟后，膝关节用金蒸气激光照射，激光能量密度100）儿旷。24 ／J'时后取膝关节滑膜作病理检查，并用脱氧核昔酸末端转移酶介导的缺口末端标记法原位检测凋亡细胞。结果： 1。模型观察：CIA大鼠炎症高峰期滑膜下炎细胞浸润明显，滑膜细胞明显增殖，炎症达到高峰后二周，血管缀形成，并侵蚀和破坏软骨和骨， CIA模型病理改变与人类RA相似。兔AIA模型膝关节滑膜病理可见滑膜细胞增生，滑膜下炎细胞浸润，也与人类RA滑膜改变相似。 2。关节周围组织中光敏剂含量的测定结果表明，各组织对HMME 的吸收速度和吸收量不同，荧光值一时间曲线不同，滑膜组织比皮肤和软骨对 HMME的吸收多，在 2 0分钟时即有明显差异。 3.PDT对CIA模型的治疗结果表明：PDT治疗后关节炎组、单纯 HMME组和治疗组踝关节左右横径统计学检验差异没有显著性，但病理评分PDT治疗组滑膜增生、血管资形成及软骨破坏、骨破坏和总分比关节炎对照组和HMME对照组好，统计学检验差异有显著性。。3＿军医进修学院硕士学位论文中文摘要 4.PDT治疗组PCNA阳性细胞较对照组少，图像分析结果表明面密度（阳性染色的面积总和与统计视野面积的比值）治疗组小于对照组，统计学检验差异有显著性。。 5.PDT治疗组凋亡阳性细胞较对照组明显增多，图像分析结果单位视野内阳性细胞数和面密度PDT治疗组高于对照组，统计学检验差异有显著性。凋亡细胞核直径PDT治疗组较小，与对照组相比，统计学检验差异有显著性。结论：二。CIA、AIA的病理改变类似人类RA，可作为研究RA病因、发病机制、检查及治疗方法的模型。 2。各组织对HMME的吸收速度和吸收量不同，滑膜组织比皮。

There were equally excellent opportunities for vacationists in the home island, delightful sylvan spots for rejuvenation, offering a plethora of attractions as well as a bracing tonic for the system in and around Dublin and its picturesque environs even, Poulaphouca to which there was a steamtram, but also farther away from the madding crowd in Wicklow, rightly termed the garden of Ireland, an ideal neighbourhood for elderly wheelmen so long as it didn't come down, and in the wilds of Donegal where if report spoke true the coup d'oeil was exceedingly grand though the lastnamed locality was not easily getatable so that the influx of visitors was not as yet all that it might be considering the signal benefits to be derived from it while Howth with its historic associations and otherwise, Silken Thomas, Grace O'Malley, George IV, rhododendrons several hundred feet above sealevel was a favourite haunt with all sorts and conditions of men especially in the spring when young men's fancy, though it had its own toll of deaths by falling off the cliffs by design or accidentally, usually, by the way, on their left leg, it being only about three quarters of an hour's run from the pillar.

在故乡的岛屿度假的人们也有同样的良机。这里有令人赏心悦目、有助于恢复青春的森林地带，都柏林市内外以及风光绮丽的近郊，不仅富于无上魅力，而且还能促进身体健康。有一条蒸气火车铁轨一直铺设到噗啦呋咔瀑布。还有威克洛那越发远离尘嚣、对&爱尔兰庭园&这一称谓当之无愧的所在。只要不下雨，那一带是供年长的人们骑自行车的理想田园，再有就是多尼戈尔的荒野，倘若传闻属实，景色也极为壮观。不过，由于最后提到的这一地区交通不便，尽管此行可获益匪浅，前往的游客毕竟有限，收入也微不足道。相形之下，霍斯山凭借绢骑士托马斯、格蕾斯。奥马利和乔治四世留下的遗迹，以及遍布于海拔数百英尺高处的杜鹃花，使它成为男女老少不分贫富，人人爱去的地方。由纳尔逊纪念柱乘车前往，只消三刻钟就可到达。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。