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It was decreased to 2.15×104 J/mol for micronized corn flour.

液化温度对细微玉米粉的葡萄糖收率没有显著影响。

In batch cultures, glucose, xylose and arabinose in cornhusk dilute acid hydrolysate after detoxification were consumed by Actinobacillus succinogenes NJ113, when the total sugar concentration was 50 g/L in medium, the final succinic acid concentration of 34.2 g/L was obtained, resulting in a succinic acid yield of 0.68 g/g and a succinic acid productivity of 0.83 g/. When the total sugar concentration was 68.2 g/L in medium, the succinic acid concentration was 42.3g/L, the yield was 0.62g/g, and productivity was 0.98 g/.

经过脱毒处理后,产琥珀酸放线杆菌 NJ113均能利用水解液中的葡萄糖、木糖、阿拉伯糖,培养基总糖浓度为50 g/L时,丁二酸分批发酵的质量收率可达0.68 g/g,浓度可达34.2 g/L,生产强度达0.83 g/,总糖浓度为68.2 g/L时,丁二酸质量收率仍可达0.62 g/g,浓度42.3 g/L,生产强度0.98 g/。

The optimal technology will provide some useful information for us to further develop Cornus officinalis.

优选的α-葡萄糖苷酶抑制剂的提取最佳工艺可以为进一步开发山茱萸提供依据。

OBJECTIVE To study the optimal extractive technology of a-glucosidase inhibitor from Cornus officinalis.

目的 研究提取山茱萸中α-葡萄糖苷酶抑制剂的最佳工艺。

Net ileal absorption of glu- cose and glutamine in the early postprandial period was decreased.

在餐后的早期时间里,大肠对葡萄糖和谷氨酸盐的吸收是下降的。

Under the optimized conditions as follows: ratio of apple pomace and cottonseed meal 1︰1, urea content 2.6%, KH2PO4 content 0.09%, glucose content 2%, initial moisture content 62.9%, fermented at 30℃ for 60 h, 5662 and 30000 U/g of xylanase and cellulase were produced, respectively.

在最佳条件(基料棉粕和苹果渣比例为1︰1,第2氮源尿素2.6%,无机盐KH2PO4 0.09%,速效碳源葡萄糖2%,含水率62.9%,30℃培养60 h)下,分别获得了5662、30000 U/g的木聚糖酶和纤维素酶的高酶活发酵干曲。

The inner diameter of b-CD cone is 0.65nm and the extra is 1.53nm. The hydroxyl groups of C(6)(the sixth carbon atoms of glucose unit and the same as follows) are located on the smaller opening of the cone with counterclockwise direction, while the hydroxyl groups of C(2) and C(3) are located on the larger opening of the cone with reverse direction.

环糊精外型是内空的圆台形,其中β-环糊精内空腔的直径为0.65 nm,外径为1.53nm.C(6)(葡萄糖单元上第六个碳,下同)上的羟基位于空腔的细口端指向逆时针方向,C(2)、C(3)上的羟基位于空腔的宽口端指向相反的方向。

Gingers seldom flowers and have seeds, so it is difficult to cultivate new variety through crossbreeding.

蔗糖比葡萄糖更利于根状茎的诱导,活性炭对根状茎诱导没有明显作用。

Objective: To study the effects of glucose, mannose and galactose on CAP10 promoter activity of Cryptococcus neoformans capsule-associated gene.

目的:研究葡萄糖、甘露糖、半乳糖对新生隐球菌荚膜相关基因CAP10启动活性的影响。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a,构建重组质粒 pET-20b-bglA 和 pET-28a-bglA,然后转化不同大肠杆菌 E.coli 宿主,Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达,重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA),可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA),比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA),这些结果表明,E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因,分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别,有助于提高该酶在 E.coli 中的表达;进一步优化诱导条件,重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养,IPTG 浓度由 1000 μM 降至 25 μM,目的蛋白可溶性表达由 12.3%增至 32.8%,提高 1.7 倍,16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达,较 37 ℃下诱导培养提高 1.2 倍。

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