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葡萄糖

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Objective To compare the effects of hypertonic electrolyte glucose solution with isosmotic electrolyte glucose solution as different resuscitation fluids on volume of fluid replacement and pulmonary vasopermeability at early stage of burn shock.

作者:沈小鹏,胡森,柴家科,邹晓防,高瑗目的比较应用1.8%的高渗氯化钠葡萄糖溶液和0.9%的等渗氯化钠葡萄糖溶液进行烧伤早期抗休克治疗对补液量及肺血管通透性的影响。

Results: Within-run CV 、day-to-day CV and total CV were 1.33%、1.42% and 2.01% respectively by iterant test; average recovery rate was 96.6%; anticoag-ulant、septic、uric acid and bilirubin under a certain concentration also did not interfere with the glucose re-sult in this method. Compared with HK method, the pertinence was Y =1.038X-1.172,r =0.994. The linearity studies showed the method was linear up to 20mmol/L.

结果: 重复性试验批内 CV 值为1.33%,批间CV 值为1.42%,总 CV 值为2.01%;回收试验中回收样品I、Ⅱ、Ⅲ的回收率分别为105%,93.5%和91.4%,平均为96.6%;干扰试验表明一般浓度的抗凝剂、防腐剂、尿酸、胆红素对葡萄糖的测定不产生干扰;比较试验,与HK法比较其相关性为Y =1.038X-1.172,r =0.994;线性范围试验表明葡萄糖浓度在20 mmol/L以下呈现良好的线性。

Results: Within-run CV 、day-to-day CV and total CV were 1.33%、1.42% and 2.01% respectively by iterant test; average recovery rate was 96.6%; anticoag-ulant、septic、uric acid and bilirubin under a certain concentration also did not interfere with the glucose re-sult in this method.

结果: 重复性试验批内 CV 值为1.33%,批间CV 值为1.42%,总 CV 值为2.01%;回收试验中回收样品I、Ⅱ、Ⅲ的回收率分别为105%,93.5%和91.4%,平均为96.6%;干扰试验表明一般浓度的抗凝剂、防腐剂、尿酸、胆红素对葡萄糖的测定不产生干扰;比较试验,与HK法比较其相关性为Y =1.038X-1.172,r =0.994;线性范围试验表明葡萄糖浓度在20 mmol/L以下呈现良好的线性。

These compounds were identified as follows:chrysophanol (FP-1),physcion(FP-2),eriosematin(FP-3),scoparone(FP-4),lupeol(FP-5),betulinic acid(FP-6),3\',4\'-Dihydroxy-trans-cinamic acid octacosyl ester(FP-7),β-sitosterol (FP-8),flemiphilippinone A(FP-9),monopalmitin(FP-10),emodin(FP-11),islandicin (FP-12),salicylic acid(FP-13),p-methoxyphenylpropionic acid(FP-14),trideca-1, 3-diene(FP-15),lupinifolin(FP-16),flemichin D(FP-17),flemiphilippinin A(FP-18), auriculasin(FP-19),erythrinin B(FP-20),6-C-prenylluteolin(FP-21), 8-(1,1-dimethylallyl) genistein(FP-22),flemiphilippinin E(FP-23),flemiphilippinin F (FP-24),5,7,3\',4\'-tetrahydroxy-6,8-diprenylisoflavone(FP-25),flemiphilippinin D (FP-26),dorsmaninsⅠ(FP-27),osajin(FP-28),6,8-diprenyleriodictyol(FP-29), lupinalbin A(FP-30),genistein(FP-31),3\'-O-methylorobol(FP-32),orobol(FP-33), 5,7,2\',3\',4\'-pentahrdroxyflavone(FP-34),the mixture of biochanin A and prunetin (FP-35 and 36),genistin(FP-37),sophororicoside(FP-38),3\'-O-methylorobol-7-glucoside(FP-39),the mixture of sissotrin and prunetin 4\'-O-β-D-glucoside(FP-40 and 41),adenosine(FP-42) and luteoloside(FP-43,mixture).

这些化合物分别为大黄酚(FP-1)、大黄素甲醚(FP-2)、eriosematin(FP-3)、滨蒿内酯(FP-4)、羽扇豆醇(FP-5)、白桦酸(FP-6)、咖啡酸二十八烷酯(FP-7)、β-谷甾醇(FP-8)、蔓性千斤拔酮A(FP-9)、单棕榈酸甘油酯(FP-10)、大黄素(FP-11)、islandicin(FP-12)、水杨酸(FP-13)、对甲氧基苯丙酸(FP-14)、十三烷-1,4-二烯烃(FP-15)、lupinifolin(FP-16)、千斤拔素D(FP-17)、蔓性千斤拔素A(FP-18)、auriculasin(FP-19)、erythrinin B(FP-20)、6-C-异戊烯基木犀草素(FP-21)、8-(1,1-二甲烯丙基)-染料木黄酮(FP-22)、蔓性千斤拔素E(FP-23)、蔓性千斤拔素F(FP-24)、5,7,3′,4′-四羟基-6,8-双异戊烯基异黄酮(FP-25)、蔓性千斤拔素D(FP-26)、dorsmaninsⅠ(FP-27)、osajin(FP-28)、6,8-双异戊烯基圣草素(FP-29)、lupinalbin A(FP-30)、染料木黄酮(FP-31)、3\'-O-甲基香豌豆苷元(FP-32)、奥洛醇(FP-33)、5,7,2′,3′,4′-五羟基黄酮(FP-34)、鹰嘴豆素甲和樱黄素的混合物(FP-35和FP-36)、染料木苷(FP-37)、槐属苷(FP-38)、7-葡萄糖基-3\'-O-甲基香豌豆苷(FP-39)、印度黄檀苷和樱黄素4′-O-β-D-葡萄糖苷的混合物(FP-40和FP-41)、腺嘌呤核苷(FP-42)和木犀草苷(FP-43,混合物)。

China rich Blueberry anthocyanins, Blueberry anthocyanins material in the pigment, anthocyanin mainly-2-glucosidase, anthocyanins-3-xylosidase, anthocyanins-3-Fu-glucoside, flowers Green-2-rhamnoside, D.-3-rhamnoside, D.-5-glucosidase, 3-o-original flower-Chui, Malvaceae pigment 3, 5-and two glucosidation 3, 3-such as stripping the original flower.

中国越橘富含花青素,越橘花青素重的色素物质,主要为花青素-2-葡萄糖苷、花青素-3-木糖苷、花青素-3-半乳糖苷、花青素-2-鼠李糖苷、翠雀花-3-鼠李糖苷、翠雀花-5-葡萄糖苷、3-O-酰原花翠素、锦葵色素-3,5-二糖甙和3,3-二-酰原花萃素等。

By the analysis of SDS-PAGE electrophoresis, CXJZ95-198 secrets exocellular proteins bands in media of glucose, mannose ,xylan, mannosan and pectin respective were 32 bands, 33 bands,35 bands, 36 bands and 21 bands respectively .

葡萄糖、甘露糖、木聚糖、魔芋粉及果胶五种不同碳源培养基中的发酵液在SDS-PAGE电泳中分别分泌出32条,33条,35条,36条和21条蛋白带;多数可比较的蛋白质电泳谱带的颜色深浅呈魔芋粉>甘露糖>木聚糖>葡萄糖>果胶的趋势。

A pectinase from CXJZ95-198 was purified. Results have made know:(1) The changes of protein content secreted by CXJZ95-198 in the five media glucose mannose xylan mannosan and pectin were 1.28mg/ml, 1.17mg/ml, 0.94mg/ml, 0.75mg/ml and 0.73mg/ml.The pectinase activity was maximum in the mannose (79.39U/ml) with the order of glucose(73.33 U/ml) mannosan(24.25 U/ml) xylan(17.45 U/ml) and pectin(15.98 U/ml).

在本研究条件下,得到如下结论:(1) CXJZ95-198菌株在以葡萄糖作为碳源的培养基中蛋白质含量变化最大,达到了1.28mg/ml,其次依次为甘露糖、木聚糖、魔芋粉和果胶培养基,分别为1.17 mg/ml,0.94 mg/ml,0.75 mg/ml,0.73 mg/ml;在这五种培养基中,以甘露糖培养基中果胶酶活性最高,达到了79.39 U/ml,其次依次为葡萄糖培养基,魔芋粉培养基,木聚糖培养基,最后为果胶培养基,分别为73.33 U/ml,24.25 U/ml,17.45 U/ml,15.98 U/ml。

It is not enough to consider separately the potential energy loss as a result of starch fermentation in the rumen for evaluating the efficiency of utilization about starch in the small intestine. Therefore, it is indispensable to evaluate the contribution of glucose absorbed from the small intestine to metabolizable glucose and the potential energy loss and healthy benefit as a result of starch fermentation in the large intestine.

评价淀粉在小肠的利用效率,不能只考虑瘤胃淀粉发酵造成的潜在能量损失,而需要评定小肠葡萄糖吸收对代谢葡萄糖的贡献及大肠淀粉发酵的能量损失和对反刍动物健康的影响。

The contents and inhibitory effects on α-glucosidase activity of saponin were significantly different among different Momordica charantia L. varieties and were higher in pulp than in seed.

不同苦瓜品种的皂苷含量及对α-葡萄糖苷酶的抑制率存在显著差异,且同一苦瓜品种果肉中的皂苷含量及对α-葡萄糖苷酶的抑制率高于籽粒。

By using β-Glucuronidase,free hesperintin and naringenin were obtained from the hesperetin-glucronide naringenin-glucronaide in plasma,the contents of the two compounds in plasma was determined by HPLC.

利用葡萄糖醛酸酶使血浆中的苷元与葡萄糖醛酸游离,用HPLC方法,测定血浆中柚皮素及橙皮素的含量,并用3p97软件进行数据分析。

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