葡萄糖

Their structures were elucidated on the basis of chemical and spectral analysis. RESULTS: Twelve compounds were isolated from the 95% ethanol extract of the aerial parts of L.rotata. Their structures were identified as luteolin (1), luteolin 7-O-β-D-glucopyranoside (2), apigenin (3), apigenin 7-O-β-D-glucopyranoside (4), apigenin7-O-6-(E-p-coumaroyl-β-D-galactopyranoside (5), tricin (6), acacetin (7), genkwanin (8), lamiophlomiol A (9), lamiophlomiol B (10), shanzhiside methyl ester(11)and syringic acid (12), respectively.

结果：从独一味地上部分的乙醇提取物中分离得到了12个化合物，分别鉴定为木犀草素(1)、木犀草素7-O-β-D-吡喃葡萄糖苷(2)、芹菜素(3)、芹菜素7-O-β-D-吡喃葡萄糖苷(4)、芹菜素7-O-（6-反式－对－香豆酰基）－β-D-半乳糖苷(5)、小麦黄素(6)、刺槐素(7)、芜花素(8)、独一味素A(9)、独一味素B(10)、山桅苷甲酯(11)及丁香酸(12)。

The purpose of the experiment is to realize the prokaryotic expression of β-glucosidase gene from Acetobacter xylinum ATCC 23769, which will be used to produce enzyme preparation so as to develop the efficiency of extracting resveratrol from plants. Aspergillus niger β-glucosidase gene is cloned both at DNA lever and cDNA lever, which will lay a foundation for further research about its expression in Saccharomyces cerevisiae in a secretory form with the aim of increasing resveratrol contents in wines.

本实验实现了木醋杆菌β-葡萄糖苷酶基因的原核表达，目的是为了生产粗酶制剂，用于提高从植物中提取白藜芦醇的得率；分别在DNA和cDNA水平从黑曲霉中克隆β-葡萄糖苷酶基因，为下一步在酿酒酵母中实现分泌表达以提高葡萄酒的白藜芦醇含量的研究奠定基础。

This method is more simple and inexpensive than screening cDNA library, and can be easily adapted to clone other genes. An Amorpha fruticosa cDNA clone encoding UDP-glucose pyrophosphorylase, a key enzyme producing UDP-glucose in the synthesis of sucrose and cellulose, was cloned by using this method.

本论文利用此方法首次从紫穗槐中克隆了与纤维素合成前体----尿苷二磷酸葡萄糖的代谢有关的尿苷二磷酸葡萄糖焦磷酸化酶基因和与木质素合成相关的4-香豆酸：CoA连接酶(4CL)基因的全长cDNA序列，并由此推测出相应的氨基酸序列。

The method takes microcrystalline cellulose as affinity column chromatographic medium of Beta-glucosaccharase; under low temperature and anacid condition, the sample of Beta-glucosaccharase is loaded and specifically adsorbed to the microcrystalline column, and then the concentration of NaCl is increased to elute the enzyme, so as to achieve high-efficient and economic separation and purification.

本方法以微晶纤维素作为β-葡萄糖苷酶的亲和柱层析介质，在低温和微酸性条件下，将β-葡萄糖苷酶上样并特异性吸附于微晶纤维素柱，然后再通过增加NaCl浓度的方法将该酶洗脱，从而达到其高效而经济分离纯化的目的。

The quantitative autoradiographic 2-(14C)-deoxy-D- glucose methods were used to assess the local cerebral glucose utilization in 46 brain structures in rats suffering from heatstroke.

利用2-(14C)-去氧葡萄糖自动放射显影定量法，分析了大白鼠热中风后脑部46个神经核的葡萄糖代谢率。

Results The highest drug use frequency was biguanide. There was 50.9% in the oral antidiabetic drug combination. Leading the first 3 places on the list of drug combination were sulfonylureas in combination with biguanide, sulfonylureas in combination with α-Glycosidace inhibitor, biguanide in combination with α-Glycosidace inhibitor, while there was 16.1% in 3 kinds combination. When oral antidiabetic drugs were combined with other drugs, combination with antihypertensives drugs led the fist, while with antihypertensives drugs led the second.

结果 二甲双胍使用频率最高，口服降糖药联合用药的比例高达50.9%，居前3位的依次是磺脲类＋双胍类、磺脲类＋α-葡萄糖苷酶抑制剂和双胍类＋α-葡萄糖苷酶抑制剂，而3种以上口服降糖药联合用药的比例也达16.1%；口服降糖药与其他药物联合使用情况看，配合使用抗高血压药的频率最高，其次是降脂药。

The thicking of secondary cell wall may be one of the reason of ethanol accumulation. The activities peak value of CAT, PPO and POD were advanced and the activity of AAO gradually increased, the content of total phenol decreased sharply, the production rate of superoxide anion ,the content of MDA and H2O2 in flesh of 'brumal jujube' increased treated with ABA during storage, this showed ABA accelerated the scenescence of fruit, the effects of ABA on 'brumal jujube'fruit was contrary to GA.

点红冬枣贮藏过程中，各有机酸(柠檬酸、t-顺乌头酸、琥珀酸、延胡索酸)含量逐渐下降，苹果酸略有增加，丙酮酸含量逐渐增加，峰值出现后下降，说明丙酮酸的增加与进入三羧酸循环的有机酸流量的降低是导致果实乙醇发酵的机制之一；点红冬枣中蔗糖含量较高，贮藏期间，蔗糖含量下降，葡萄糖和果糖含量增加，气调贮藏可延缓果实蔗糖降解和葡萄糖和果糖含量的增加。

AIM: To investigate the morphological changes of apoptosis in human colon cancer cell line SW1116 induced by 2 ［(3 carboxy 1 oxoprogy1) amino］ 2 deoxy D glucose.

目的：研究D 葡萄糖衍生物2 （3 羧基 1 丙酰氨基） 2 脱氧 D 葡萄糖诱导人结肠癌细胞SW1116凋亡时，其形态学的变化。

Glucose-6-phosphatase system (G6PA) is the key enzyme that catalyses the reaction glucose production in cells, which is composed by 5-6 subunits.

葡萄糖-6-磷酸酶（G6PA）是催化细胞内产生葡萄糖的关键酶，此酶由5-6个亚基构成。

RESULTS:12 compounds separated from Dendranthema nankingense were elucidated as cerotic acid,β-sitosterol,ursolic acid,chrysoeriol,luteolin,caffeic acid,daucosterol,chrysoeriol-7-O-glucoside,luteolin-7-O-glucoside,linarin,rutin and potassium nitrate.

结果：从菊花脑中分离得到12个化合物，即正二十六烷酸、β-谷甾醇、熊果酸、金圣草黄素、木犀草素、咖啡酸、胡萝卜苷、金圣草黄素-7-O--βD-葡萄糖苷、木犀草素-7-O--βD-葡萄糖苷、蒙花苷、芦丁、硝酸钾。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。