葡萄球菌的

Results In 585 staphylococcus, there were 406 staphylococcus aureus, which including 387 methicillin sensitive staphylococcus aureus and 19 methicillin resistant staphylococcus aureus. And there were 179 coagulase negative staphylococcus, including 20 methicillin sensitive coagulase negative staphylococcus and 159 methicillin resistant coagulase negative staphylococcus.

结果 585株葡萄球菌中，金黄色葡萄球菌406株，其中甲氧西林敏感的SA387株，甲氧西林耐药的SA19株；凝固酶阴性的葡萄球菌179株，其中甲氧西林敏感的CNS20株，甲氧西林耐药的CNS159株。

Results Among 70 strains of Staphylococcus spp., 56 strains were positive Staphylococcus coagulase gene with PCR; the same 56 strains were also positive with latex agglutination test; 54 of which were positive with tube test method, 2 strains were false negative; strong positive were detected with slide method, all these 56 strains were identified as Staphylococcus aureus by VITEK-Ⅱ automatic analyzer.

结果 70株葡萄球菌属细菌中，PCR法检测葡萄球菌凝固酶基因56株为阳性；Slidex Staph Plus与PCR法相对应的56株为阳性；试管法检测与前2种方法相对应的54株为阳性，2株为假阴性；玻片法为强阳性；VITEK-Ⅱ全自动分析仪鉴定56株为金黄色葡萄球菌

Besivance is indicated for the treatment of bacterial conjunctivitis in patients aged ≥1 year whose infection is caused by susceptible isolates of the following bacteria: CDC coryneform group G, Corynebacterium pseudodiphtheriticum, Corynebacterium striatum, Haemophilus influenzae, Moraxella lacunata, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus lugdunensis, Streptococcus mitis group, Streptococcus oralis, Streptococcus pneumoniae, and Streptococcus salivarius.

Besivance适用于治疗年龄≥1 岁、由下列细菌的致病分离物所致的细菌性结膜炎患者：CDC棒状杆菌G群，假白喉棒杆菌，纹带棒杆菌，流感嗜血杆菌，腔隙莫拉菌，金黄色葡萄球菌，表皮葡萄球菌，人葡萄球菌，路邓葡萄球菌，缓症链球菌群，口腔链球菌，肺炎链球菌以及唾液链球菌。

Hematolytic staphylococcus, staphylococcus epiderrnidis, torpidity staphylococcus, squirrel staphylococcus and mimicry staphylococcus were the top five pathogenic bacteria.

结果 在所进行的8220次细菌学检测中共发现1320株阳性细菌，其中发现202株CNS，占总体检出率的15.3％（202／1320），其中溶血葡萄球菌、表皮葡萄球菌、缓慢葡萄球菌、松鼠葡萄球菌、模仿葡萄球菌等为主要的前5位致病菌。

Methods S. epidermidis atlE gene expression level at different phases was detected by RT-PCR, and the accumulation of extracellular DNA at different phases was assessed by spectrophotometric measurements of light absorbance by DNA; DNase Ⅰ was used to study the function of extracellular DNA in biofilm formation and primary attachment. The effects of atlE gene deletion on initial adherent capacity, biofilm formation and extracellular DNA release were studied by construction of atlE deletion mutant via homologous recombination.

采用RT-PCR法检测表皮葡萄球菌atZE基因在不同时期的表达水平，用紫外分光光度计检测DNA的方法检测了相应时期的胞外DNA的释放量，并用DNA酶研究表皮葡萄球菌胞外DNA在生物膜形成和起始黏附中的作用；采用ρBT2质粒同源重组敲除的方法构建了表皮葡萄球菌1457的atlE基因突变株，研究atlE基因敲除突变对起始黏附能力、生物膜形成及胞外DNA释放能力的影响。

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

We have investigated the conformational features of four short peptide fragments of Staphylococcal nuclease by homonuclear two-dimensional NMR and circular dichroism with a view to examining the early events in the folding process of SNase.

中文题名金黄色葡萄球菌核酸酶折叠起始位点：N-末端短肽段的NMR研究副题名外文题名 Folding initiation sites of staphylococcal nuclease：exploring the solution conformation of N-terminal fragments by nuclear magnetic resonance 论文作者戴继勋导师王金凤研究员学科专业分子生物学研究领域\研究方向学位级别博士学位授予单位中国科学院生物物理研究所学位授予日期2001 论文页码总数68页关键词金黄色葡萄球菌核酸酶蛋白质折叠核磁共振馆藏号BSLW /2001 /Q71 /89 本论文工作用四个金黄色葡萄球菌核酸酶N-末端短片段作为新生肽链折叠的模型，用同核二维核磁共振谱和远紫外圆二色谱研究了它们的溶液构象特征。

Some retrospective studies have proved that the clinical situations of Panton-Valentine leukocidin一producing Staphylococcus aureus infections are distinctive,especially necrotizing pneumonia caused by PVL-positive S aureus seems to be epidemic CA—MRSA stains causing skin and soft tissue infections,necrotizing pneumonia and lethal necrotizing fasciitis.

呼吸系统论文：近年来，国外对葡萄球菌杀白细胞素进行了大量的研究，多数研究显示产金黄色葡萄球菌感染有着特有的临床表现，特别是产PVL金黄色葡萄球菌相关性坏死性肺炎可能为一新现的临床疾病，由此PVL在金黄色葡萄球菌感染中的致病研究日益受到重视。

Objective To establish a sensitive and specific gene probe method for detection of Staphylococcal enterotoxin B gene.

目的 建立一种灵敏度高、特异性好的DNA 探针检测葡萄球菌肠毒素B 基因方法。方法用聚合酶链反应扩增的方法进行葡萄球菌B 型肠毒素基因探针生物素标记，用制备好的基因探针，进行斑点杂交，对葡萄球菌肠毒素10 株标准株和39 株临床株分别进行了检测。

Lysostaphin, a specific endopeptidase enzyme derived from Staphylococcus aureus, is a bactericidal agent against Staphylococcus and difficult to be drug-resistant.

溶葡萄球菌酶能够特异性杀灭金黄色葡萄球菌且不易产生耐药性，有望成为治疗葡萄球菌属细菌引发感染的特效药物。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。