葡萄球菌的

In particular, these compounds are superior to the existing cephem derivatives of the onium salt type in the antibacterial activities on methicillin-resistant Staphylococcus aureus, penicillin-resistant Streptococcus pneumoniae and imipenem-resistant Pseudomonas aeruginosa, which makes them highly useful as remedies for infectious diseases caused by various pathogenic bacteria.

特别是，它具有比现有的__盐型头孢烯衍生物更高的对抗甲氧苯青霉素抗性的金黄色葡萄球菌、青霉素抗性的肺炎链球菌和亚胺培南抗性的绿脓杆菌的抗微生物活性，并因此可非常有效地作为各种病原菌衍生的传染病的治疗剂。

Material and method the 1.1 objects that monitor in January 2005 ~2007 year in December, nantong city disease accuses a center to monitor bar of hall of inn of the inn inside the area, communal bathroom, tonsorial hairdressing, song to wait public 771, every random and draw-out a certain number of the sample of public articles for use that the preparation after cleaning disinfection uses in all 4 960 portion. Crock of pool of the tea service of the 1.2 content that monitor and methodological inn, towel, bed sheet, face, bath, chamber pot undertakes group testing of bacterial gross, coliform measures; the towel of communal bathroom, bathrobe undertakes group testing of bacterial gross, coliform measure, slipper undertakes mould and microzyme detect the tea service of; public place of entertainment undertakes group testing of bacterial gross, coliform measures; the towel of tonsorial hairdressing place, scissors undertakes bacterial gross, coliform group detect with coccus of golden yellow grape.

梗概： 为了解南通市近几年公共场所卫生状况，客观评价公共场所卫生水平，为今后的卫生监督监测提供科学依据，对2005年~2007年南通市公共场所公共用品(茶杯、口杯、床上用品等)的细菌总数、大肠菌群等微生物检测进行了分析，结果报告如下。1材料和方法1.1监测对象2005年1月~2007年12月，南通市疾控中心监测区内的旅店、公共浴室、理发美容店、歌厅酒吧等公共场所771家，每家随机抽取若干件清洗消毒后预备使用的公共用品样品共4 960份。1.2监测内容和方法旅店的茶具、毛巾、被单、面池、浴缸、便桶进行细菌总数、大肠菌群检测；公共浴室的毛巾、浴衣进行细菌总数、大肠菌群检测，拖鞋进行霉菌和酵母菌检测；娱乐场所的茶具进行细菌总数、大肠菌群检测；理发美容场所的毛巾、剪刀进行细菌总数、大肠菌群和金黄色葡萄球菌检测。

The book comprehensively reviews the molecular and cellular biology of the most important microbial toxins with a focus on recent advances in the field, covering topics such as toxin ADP-ribosylation of proteins, toxin inhibition of protein synthesis, modulation of actin cytoskeleton, cytolethal distending toxins, cell-damaging/pore-forming toxins, clostridial neurotoxins, toxins of the immune system, streptococcal and staphylococcal toxins, anthrax toxins, Shiga toxin, Yersinia toxin, mycotoxins, and the regulation of toxin expression, as well as the potential of microbial toxins in bioterrorism.

该书全面评论了最重要的微生物毒素的分子与细胞生物学，重点是这一领域中的最新进展，主题包括蛋白质毒素ADP核糖基化，毒素抑制蛋白质合成，肌动蛋白细胞骨架的调控，细胞致死增加毒素，细胞损伤/孔状毒素，梭菌属的神经毒素，免疫系统的毒素，链球菌和葡萄球菌毒素，炭疽毒素，Shiga毒素，Yersinia毒素，毒枝菌素，和毒素表达调节，以及生物恐怖中微生物毒素的潜在可能性。

The results showed that the main pathogenic bacteria ledlocal cows to endometritis were streptococcus,the next,Escherichia coli,staphylococcus and pseudomonas aeruginosa.NFPL could quickly reduce thenumber of the bacteria in uterus,and strongerly inhibit these bacteria in vitro.NFPL could excit the uterus,which occurred in enlarging contractionamplitude.NFPL could lower abnormal high content of progesterone and raiseabnormal low oestradiol.

结果表明，引起本地奶牛子宫内膜炎的主要致病菌是链球菌，其次是大肠埃希氏杆菌，葡萄球菌，绿脓杆菌；新促孕液能能迅速减少病牛子宫内细菌的数量，在体外对这些细菌也有较强的抑制作用；新促孕液对兔子宫有明显的兴奋性作用，主要表现在收缩波幅的加大；新促孕液能降低病牛血清异常升高的孕酮含量，升高异常降低的雌二醇含量。

The average kill rate against natural bacteria on kitchenware, such as table top, chopping block, knife, cupboard, porcelain bowl and the surface of refreshing box of the refrigerator were all above 0.972 0 when cleaning and polishing with vinegar solution for 10 min.

结果 不同浓度的食醋溶液作用不同时间对金黄色葡萄球菌、大肠杆菌和白色念珠菌的杀灭效果不同，浓度越大、作用时间越长杀菌效果越好（F=6.89～156.83，P.05）；对手自然菌作用2 min，平均杀灭率为0.991 5±0.003 2；对厨房内台面、砧板、菜刀、碗柜、瓷碗、冰箱保鲜室表面擦拭消毒10 min，对自然菌的平均杀灭率在0.972 0以上；对室内空气自然菌的杀灭率随熏蒸时间的延长而提高（F=121 2.13、502 4.46，P.05），当室内有人时，杀灭率有所降低（t=5.08～30.48，P.05）；置于54 ℃恒温箱（相对湿度≥0.75）存放14 d，醋酸总含量下降

Results Different concentrations of vinegar with different disinfection duration showed significantly different bactericidal effect on Staphylococcus, Escherichia and Candida albicans (F=6.89~156.83, P.05). The effect was better along with higher concentration and longer duration. The average kill rate against natural bacteria on hands was 0.9915±0.0032 after exposed to vinegar solution for 2 min. The average kill rate against natural bacteria on kitchenware, such as table top, chopping block, knife, cupboard, porcelain bowl and the surface of refreshing box of the refrigerator were all above 0.9720 when cleaning and polishing with vinegar solution for 10min. The bactericidal efficacy of vinegar against natural bacteria in room air was enhanced with fumigating duration prolonged (F=1212.13, 5024.46; P.05), while lowered when there were persons in it (t=5.08-30.48, P.05). The content of acetic acid in vinegar dropped by 8.372 g/L when it was stored at 54℃(relative humidity 0.75) for 14 days.

结果 不同浓度的食醋溶液作用不同时间对金黄色葡萄球菌、大肠杆菌和白色念珠菌的杀灭效果不同，浓度越大、作用时间越长杀菌效果越好(F=6.89~156.83，P.05)；对手自然菌作用2min，平均杀灭率为0.9915±0.0032；对厨房内台面、砧板、菜刀、碗柜、瓷碗、冰箱保鲜室表面擦拭消毒10min，对自然菌的平均杀灭率在0.9720以上；对室内空气自然菌的杀灭率随熏蒸时间的延长而提高(F=1212.13、5024.46，P.05)，当室内有人时，杀灭率有所降低(t=5.08~30.48，P.05)；置于54℃恒温箱（相对湿度≥0.75）存放14d，醋酸总含量下降

The results showed that the three disparate treated substances have no visible antibacterial effect to E. coli , but a obvious effect to Staphylococcus aureus with inhibition of about 10 bacteriostasis units; have dissimilar effects to 10 kinds of fungi among the total kinds, and have a good inhibitory effect to Cytospora.coccodesHughes with fungistasis rate above 65%. After the gel filtration and activity detection, 50mM ammonium acetate was screened out as the more suitable eluents, the crudes were separated into strikingly unlike parts with obviously different antimicrobial activity.

结果表明，大蜡螟抗菌物质对大肠杆菌没有明显的抑制作用，而对金黄葡萄球菌抑制作用明显，抑菌活性单位在10左右；对10种植物病原真菌均有不同的抑菌活性，其中对苹果树皮腐烂病菌的抑制率达到65%以上；经过凝胶过滤分离，及对分离组分的抑菌活性测定，筛选出较合适的洗脱液为50mM乙酸铵，各分离组分抑菌活性差异明显，粗提物被分成了两个差异显著的部分。

Methods:(1) Dissoluble PGN and CpG DNA were immobilized onto the surface of biotin cuvette for establishing target. Another effective tracking approach was established by immobilizing Escherichia lipid A F583 onto the surface of Non-derivatised cuvett. The biosensor technology was applied to screen anti-inflammatory TCM targeting on three key molecules.(2) The active compositions were isolated by AB-8 macroreticular resin from lycium bark. After the activities of compositions were evaluated, the most effective compositions was confirmed. In vitro, the affinities of different concentrations composition E binding with PGN, CpG DNA and lipid A were measured separately. The effect of composition E on vigor of RAW264.7 cells were tested by MTT and CCK-8, and its inhibition on TNF-α, which was released from RAW264.7 cells induced by PGN, CpG DNA and LPS, was also tested by ELISA. In vivo, murine sepsis models were made by intravenously heat-killed E.coli and heat-killed S.aureus, then protection of composition E on mice sepsis model were observed.

(1)将PGN及CpG DNA包被于生物素样品池，将lipid A包被于非衍生样品池，分别建立以PGN、CpG DNA及lipid A为靶点的技术平台，对114种抗炎中药水提物进行筛选、评价其活性物质含量，并评估出针对上述三种病原分子均具有较高结合活性的中药；(2)利用生物传感器跟踪检测技术、大孔吸附树脂分离技术，从地骨皮中定向分离与PGN、CpG DNA及lipid A均具有较高亲和力的活性组分；在体外实验中，测定不同浓度活性组分与PGN、CpG DNA及lipid A亲和力；MTT法及CCK-8法检测活性组分对RAW264.7细胞活力的影响；ELISA法检测活性组分对PGN(2μg/ml)、CpG DNA(10μg/ml)及LPS(100ng/ml)刺激小鼠RAW264.7细胞分泌TNF-α的抑制作用；在体内实验中，采用尾静脉注射致死剂量热灭活大肠杆菌和热灭活金黄色葡萄球菌，建立细菌脓毒症小鼠模型，观察活性组分对脓毒症模型小鼠的保护作用。

Methods Five hundred and four specimens were isolated from the wounds and nasal vestibules of burn patients ,the hands and nasal vestibules of medical staffs and lay attendants and the surfaces of various equipments. From these specimens,58 strains of MRSA and 43 methicillin- sensitive staphylococcus aureus were isolated. The genome DNA of isolated MRSA strains was analyzed by repetitive DNA - sequence- based PCR analysis.

方法采集烧伤患者的创面、鼻前庭，工作人员手、鼻前庭，陪护家属的手、鼻前庭及烧伤科病房各种环境表面共504份标本，从中分离到MRSA 58株，对苯唑西林敏感的金黄色葡萄球菌43株，并对所分离的MRSA菌株的基因组DNA进行重复序列PCR检测。

Keeping this in mind, a study was undertaken to determine the effect of CA on early growth of mung bean (Phaseolus aureus=Vigna radiata), morphogenetic response in hypocotyl cuttings of mung bean, and changes in the activities of some associated enzymes – proteases, polyphenol oxidases, and peroxidases – and endogenous total phenolics at root initiation (third day; 48 h; appearance of root primordia; RI), root expression (fifth day; 120 h; appearance of roots; RE), and post-expression (seventh day; 168 h; PE) stages of rhizogenesis.

保持这一点，一项研究，以确定影响钙对早期生长绿豆（ phaseolus金黄色葡萄球菌=豇豆拉迪雅塔），形态发生反应，下胚轴插条绿豆，和变化，在活动的一些相关的酶-蛋白酶，多酚氧化酶，过氧化物酶-和内源性总酚的从根本上启动（第三天； 4 8小时；外观根原基；里），根的表达（第五天； 1 20改为H级；外观根；转口），和后表达（第七天； 168改为H级； PE ）的阶段rhizogenesis 。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。