葡萄球菌的

93 Target gene were detected by Tem-PCR from 75 specimen, they were: Hemophilus influenzae 40, Streptococcus penumoniae 36, Acinetobacter baumannii 10, Pseudomonas aeruginosa 4, Staphylococcus aureus 3, the other 9 kinds of bacterium including Escherichia coli、Klebsiella pneumoniae and Enterobactor cloacae were not detected by Tem-PCR, the positive rate of Tem-PCR was 39.9%(75/188).(3) For the 14 kinds of bacterium designed by Tem-PCR, compared with the culture, the sensitivity、specificity and coincidence of Tem-PCR is 51.0%, 68.0%, 58.3% respectively.

2经Tem-PCR技术扩增后，188例标本在Luminex100多功能悬浮点阵仪中有75例呈阳性，共检测出93株病原菌的靶基因，分别是流感嗜血杆菌40株，肺炎链球菌36株，鲍曼氏不动杆菌10株，铜绿假单胞菌4株，金黄色葡萄球菌3株，另外9种Tem-PCR已设计的细菌包括肺炎克雷伯菌、大肠杆菌、阴沟肠杆菌等均未检出，Tem-PCR的阳性率是39.9%(75/188)。

Methicillin-resistant Staphylococcus aureus has become the most common cause of skin and soft tissue infections, taking emergency room doctors back to the pre-penicillin days, when skin infections were lanced and drained, according to a new study.

根据一项最新研究，甲氧苯青霉素抵抗的金黄色葡萄球菌已经成为皮肤和软组织感染最常见的原因，当皮肤感染被切开和引流之后，将急诊科医生带回了无青霉素的年代。

Staphylokinase is a 136-amino acid protein,secreted by certain strains of lysogenesis Staphylococci with thrombolytic potential.

葡激酶是由溶原性葡萄球菌分泌的一种具有潜在溶栓功能的含有136个氨基酸的蛋白质。

Methods The bacteria and the yeast S.aureus, E.coli and C.albicans in different dilute concentration were determined by direct microscopy and isolation to observe the positive rate of microbiologic diagnosis.

方法将不同稀释度的金黄色葡萄球菌、大肠埃希菌、白色念珠菌的菌液涂片革兰染色镜检和分离培养，观察标本中细菌或真菌检出的情况。

The in vitro and in vivo activity of these specific IgY and their opsonization to phagocytes were estimated.Specific IgY were produced by hens immunized with formaldehyde killed S.aureus or E.

本研究制备了抗奶牛乳腺炎主要致病菌——金黄色葡萄球菌和大肠杆菌特异性卵黄抗体，考察了抗体的体外抑菌活性、对吞噬细胞的吞噬调理作用及对奶牛乳腺炎的治疗作用。

Through comparison of 16S rRNA gene sequence of Riemerella anatipestifer serotype 1 to 19 with that of Escherichia coli,Salmonella,Pasteurella and Staphylococci,a pair of specific primers was designed.

根据 GenBank中的鸭疫里默氏杆菌1～19型16S rRNA基因序列，分别与大肠杆菌、沙门氏菌、葡萄球菌、巴氏杆菌的16S rRNA基因序列对比，设计特异性引物，建立了基于鸭疫里默氏杆菌16S rRNA的PCR检测方法。

Secondly, the rate of infection of the Pasteurella multocida reached to 16%(12/75), Riemerella anatipestifer bacilliis also high, the ratio was 12%(9 / 75). More over, hemolytic bus bacteria, Salmonella and Staphylococcus aureus were also showed in the poultry tested.

结果表明：大肠杆菌仍是该部分地区危害家禽的主要致病菌，它的个体感染率高达41.3%（31/75）；其次是多杀性巴氏杆菌，个体感染率为16%(12/75)；鸭疫里默氏杆菌的个体感染率也较高，为12%（9/75）；此外还分离到溶血性巴氏杆菌、沙门氏菌和葡萄球菌。

Among pathogens , the most common pathogens associated with VAP were Pseudomonas aeruginosa ( 3013 %), Acinetobacter baumanii (1115 %), Klebsiella pneumoniae ( 1017 %), Escherichia coli ( 716 %) and S taphylococcus aureus ( 618 %). Their multiple drug2resistance to antibiotics was serious. Drug sensitivity tests indicated that the GNB had lower drug2 resistance to such antibiotics as imipenem , amikacin , piperacillin/ tazobactam , ceftazidime , cefepime , cefoperazone/sulbactam , ciprofloxacin and ticarcillin/ clavulanate. Polymicrobial pathogens were more isolated in late2onset VAP than in early2onset VAP.

结果 共检出致病菌383 株，革兰阴性细菌占8411 %，其中非发菌占5114 %，肠杆菌科占3216 %，革兰阳性球菌占1218 %，其中MRSA和MRSE 占615 %，真菌占311 %；前5 位致病菌分别为铜绿假单胞菌(3013 %)、鲍氏不动杆菌(1115 %)、肺炎克雷伯菌(1017 %)、大肠埃希菌(716 %)、金黄色葡萄球菌(618 %)等；药敏结果显示这几类菌株的多重耐药现象严重，较为敏感的抗菌药物有亚胺培南/西司他丁、阿米卡星、哌拉西林/他唑巴坦、头孢他啶、头孢吡肟、头孢哌酮/舒巴坦、环丙沙星及替卡西林/克拉维酸等，革兰阳性细菌均对万古霉素敏感；迟发性VAP 的混合培养比例显著高于早发性VAP。

Three classes, forty-eight in total, of pyrazole compounds, imidazolo[1,2-b] pyrazoles, dipyrazolylmethanones and pyrazolyloxadiazoles, have been synthesized, and they have been fully characterized by modern spectroscopic methods, two of which were further confirmed by X-ray diffraction. Most of these compounds have been evaluated for the inhibitions of Gram-positive bacterium represented by Staphylococcus aureus and Gram-negative bacterium represented by Klebsiella pneumoniae.

论文合成了咪唑并[1，2-b]吡唑类、二吡唑基甲酮类和吡唑连噁二唑类共三类48个化合物，使用了现代结构测试手段对所合成的化合物进行了结构表征，其中两个化合物经过了X-Ray射线衍射分析，并对其中大部分化合物进行了以金黄色葡萄球菌和肺炎克雷白杆菌为代表的革兰氏阳性菌和革兰氏阴性菌的初步抑菌活性测试。

Results: The essential oil from the fresh Houttuynia Cordata Thunb possessed the highest antibacterial activity against Staph.aureus and Sarcina,and the MIC was 1/2 х 10(-3) ml/ml.

结果：鱼腥草鲜样挥发油的抑菌效果最强，对金黄色葡萄球菌、八叠球菌的MIC均为1/2×10-3ml/ml，在贮藏过程中随着时间的延长MIC逐渐增大。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。