葡萄球菌溶素

in order to establish a rapid, sensitive and specific detection method for identification of four kinds of food-borne pathogenic bacteria including salmonellla spp, listeria monocytogenes, staphylococcus aureus and bacillus cereus by using multiplex pcr, according to invasion protein a geneof salmonellla spp, transcriptional regulatory protein gene of listeria monocytogenes, autolysin gene ofstaphylococcus aureus and gyrase b subunit gene of bacillus cereus, four pairs of specific primers were designed for multiplex pcr amplification.

小 摘要：为建立一种利用多重pcr技术检测和鉴定沙门氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌和蜡样芽孢杆菌的方法，根据沙门氏菌侵袭力蛋白a基因、单核细胞增生李斯特菌蛋白转录调控基因、金黄色葡萄球菌自溶素基因、蜡样芽孢杆菌的促旋酶b亚单位基因设计引物，进行多重pcr扩增，并对多重pcr反应体系进行优化。

The secretion of δ-hemolysin well reflects the regulation of access gene regulation system in S. aureus.

δ溶血素的分泌反映了金黄色葡萄球菌密度感应系统调控外毒素分泌的特点。

But we have seldom found the reports about it in China; So writer summarized some literature more than twenty years; name,classification and family of hemolysin have been written, especially three of representative species:α-toxin, Streptolysin O and HlyA; Nowaday, A relationship between the hemolysin and pathopoiesis of bacterium is obvious.

另外，细菌溶血素的英文命名并不统一，检索时容易造成漏检或误检；通过查阅大量英文文献资料，归纳汇总近20年报道的溶血素研究进程；该文详细列出了细菌溶血素名称、分类以及各溶血素家族列表。并且分别介绍了其中三种典型的穿孔毒素：葡萄球菌α-毒素、链球菌溶血素-O、埃希氏大肠杆菌溶血素HlyA。

Methods Three pairs of primers have been designed according to the hilA of Salmonella spp., hemolysin of Proteus mirabilis and Staphylococcus aureus clone pSa-442 Sau3AI fragment and the anticipated PCR products were 580bp, 401bp and 256bp.

本研究依据沙门菌侵袭基因正调节蛋白基因、变形杆菌溶血素基因和金黄色葡萄球菌特异性pSa-442序列，运用Primer Premier 5.0分别设计3对特异性引物，预计PCR扩增的目的基因片段分别为为580bp、401 bp、256 bp。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。