葡萄球菌

Results totally 287 strains were isolated from the 256 positive samples , and the gram-negative bacilli were 225(78.4%,), the gram-positive coccus were 41(14.3%), snd the monilia were 21(7.3%).the distributions of clinical bacteria were respiratory tract(63.4%),urinaryract(7.0%),secretion(includingwound .3%),blood(5.9%),stool(5.2%), pucture fluid(4.9%), and other sites(7.3%). of all isolating bacterium,from the first to the fifth were ps.aeruginosa(19.5%),k.pneumoniae(16.7%), e.coli(14.3%), a.baumannii(11.8%) and psemal (10.1%).resistant rates of methecillin-resistant s.aureus,methecillin-resistant coagulase-negative staphylococci and vancomycin-resistantwere 88.2%、70.0% and 11.1% respectively;the incidence of e.coli and k.penumoniae produce extended speutrum beta-lactamase were 68.6%和65.2%, 44.6% of ps.aeruginosa isolates were resistant to imipenem; the highest examining rate of 21 kinds of monilia was candida albicans (66.7％),resistant rate of candida albicans to fluconazole and amphotricin b was 51.3％ and 1.3%.

结果 在254份检出细菌阳性标本中共培养出287株细菌，其中革兰阴性杆菌225株（78.4％），革兰阳性球菌41株（14.3%），念珠菌21株（7.3%），检出菌来自呼吸道标本占63.4%，其他标本各占5%左右；细菌检出占构成比前三位的依次为铜绿假单胞菌19.5%、肺炎克雷伯菌16.7%、大肠埃希菌14.3%；耐甲氧西林金黄色葡萄球菌、耐甲氧西林凝固酶阴性葡萄球菌和耐万古霉素的肠球菌的发生率分别为88.2%、70.0%和11.1%；大肠埃希菌和肺炎克雷伯菌的超广谱β-内酰胺酶的检出率分别为68.6%和65.2%，铜绿假单胞菌对亚胺培南的耐药率为40.2%；白色念珠菌对氟康唑的耐药率为81.3％，对两性霉素的耐药率为3.2%。

The SPA-rich Staphylococcus aureus coated with the specific IgG was used as nephelometric immunoassay reagent and could lead to good results. On the basis of nephelometric immunoassay combined with immunomagnetic separation, the rapid automatic quantitative immunoassay for the detection of Shigella flexneri was established, which provides a new way for the rapid diagnosis and automatic quantitative analysis of pathogenic bacteria.

以福氏志贺菌的多抗血清分别致敏SPA包被的磁珠和富含SPA的金黄色葡萄球菌，制备特异的多抗磁珠对福氏志贺菌有一定的特异捕获与富集作用，抗体致敏的金黄色葡萄球菌作为协同凝集试剂与免疫比浊试剂对福氏志贺菌的自动、快速、定量检测具有较好的效果，免疫比浊分析结合免疫磁分离技术为病原菌的快速诊断和自动定量分析提供了新的思路。

Another method that has been proposed to identify S.aureus from nonclinical sources employs an immunoenzymatic assay using a monoclonal antibody assay using a monoclonal antibody prepared against S.aureus endo-β-acetyl-glucosaminidase, an enzyme produced by all isolates of this species.

另一种方法，提出了确定非临床源金黄色葡萄球菌从员工的免疫酶检测用单克隆抗体检测用单克隆抗体制备对金黄色葡萄球菌内切β-乙酰氨基葡萄糖苷酶，这种酶产生的所有分离这一物种。

The most common potential pathogen, coagulase-negative staphylococci, were found on all of the keyboards. Diphtheroids were found on 80% of keyboards, Micrococcus species on 72%, Bacillus species on 64%, nonfermentative Gram-negative rods on 36%, vancomycin-sensitive Enterococcus on 12%, Staphylococcus aureus on 4%, and oxacillin-susceptible S aureus on 4%.

在所有键盘中都发现了最常见的致病菌凝固酶阴性葡萄球菌。80％的键盘带有类白喉菌，72%带有微球菌属，64%带有芽胞杆菌属，36%带有非发酵的革兰氏阴性杆菌，12%带有万古霉素敏感肠球菌，4%带有金黄色葡萄球菌，4%带有甲苯异恶唑青霉素易感链球菌。

The Company's products in development includes NicVAX,, a proprietary investigational vaccine for the treatment of nicotine addiction and prevention of smoking relapse; and PentaStaph (Pentavalent S.aureus Vaccine), a pentavalent vaccine designed to prevent S.aureus infections, including those infections caused by antibiotic-resistant strains of S.aureus.

该公司的产品在发展包括NicVAX ，，专有的研究性疫苗，用于治疗尼古丁成瘾和复吸预防吸烟和PentaStaph ，一个五价疫苗旨在防止金黄色葡萄球菌感染，包括那些感染耐药株金黄色葡萄球菌。

The most common microbes causing infections in percutaneously inserted uncuffed catheters are, in order of prevalence, coagulase-negative staphylococci, Staphylococcus aureus, Candida species, and enteric gram-negative bacilli.

根据患病率的高低，引起经皮插入的无气囊导管感染的最常见的微生物排序如下：凝固酶阴性葡萄球菌、金黄色葡萄球菌、念珠菌属菌种和肠道革兰氏阴性细菌。

The results showed that 0.01,0.05 and 0.10 g/ml extract of Saxifraga stolonifera Meerb had strong antimicrobial effect on S.aureus,but 0.25 and 0.50 g/ml extract had promotive effect on S.aureus.And the extract of Saxifraga stolonifera Meerb of different concentration had antimicrobial effect on E.coli.

结果表明，0.01、0.05、0.10g/ml 3种较低浓度的提取物对摇床培养的金黄色葡萄球菌生长具有较强的抑制作用，而0.25和0.50g/ml 2种较高浓度的提取物对摇床培养的金黄色葡萄球菌生长有较强的促进作用；这5种浓度的提取物均对摇床培养的大肠杆菌生长表现为抑制作用。

The drug susceptibility testing indicates that Quinotones, Amikacin,Kita sermonic, Cephazolin and Erythromycin have good inhibitory effect on Staphylococcus. All these three kinds of Staphylococcuus take on high sensitive effect.

药敏试验表明，喹诺酮类药物、丁胺卡那、庆大霉素、头孢唑啉钠和红霉素对葡萄球菌的抑制效果较好，三种葡萄球菌对上述药物均呈现高敏反应。

Objective To investigate antimicrobial resistance and Staphylococcal cassette chromosome mec genotype characteristics of methicillin-resistant Staphylococcus aureus in Southern China.

目的 了解华南地区耐甲氧西林金黄色葡萄球菌的耐药性及其葡萄球菌盒染色体的基因型特点。

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制，酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力，酶蛋白亚基间的相互作用，酶蛋白的折叠以及内运动状态与酶活力的关系，酶蛋白发挥功能时的结构特性；运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性，热稳定性机制；确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位；确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构；确定了一个新型的染色质蛋白Cren7的溶液三维结构；正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。