葡萄球菌

Extracts from 6 species of IPomoea batatas Lam leaves have been determined for their bacteriostatic effects on five types of pathogenetic bacteria (Escherichia coli,Bacillus subtilis, staphylococcus aureus, staphylococcus albus, and Proteus vulgaris) by using the methods of the filter paper disk test s and the dual-flat tests.

用滤纸圆片法和双层平板法测试六种不同品种红薯叶（SPGS—1，SPGS—2，SPGS－3，SPGC，SPRC，SPRS）的提取物对五种致病菌：大肠杆菌、枯草杆菌、金黄色葡萄球菌、白色葡萄球菌、普通变形杆菌的抑制作用、结果发现SPGS—1、SPGS—2、SPGS—3和SPRS四种红薯叶提取物有明显的抑菌作用，其最低抑制浓度范围为0.4g／ml～1·0g／ml

The method of the soluble peptidoglycan isolation by short dose benzylpenicillin sodium and sephadex G-100 was founded; through the SLP reagent kit, the limulus test and the improved LOWRY reagent kit detecting, the prepared soluble peptidoglycan include shorter LPS and lower protein, its purity coefficient reach 93%.

建立并完善了青霉素诱导和Sephadex G-100凝胶柱法制备金黄色葡萄球菌可溶性肽聚糖的方法；经SLP试剂、鲎试验及改良的LOWRY试剂检测，所制备的金黄色葡萄球菌可溶性肽聚糖中LPS和蛋白的污染均较少，纯度达到93%以上； 3。

Alloy of polyethylene of element of the methodological freeboard that make, titanium and sample of alloy of cobaltic chromic molybdenum, FITC of classics of cuticular grape coccus labels, the cuticular grape coccus that after sample of artificial and articulatory material is disinfected, vaccinal FITC labels, sample surface cent is slick surface and rough surface, every groups each 6 sample, brood the 24 orifice plate that contain bacterium and sample below 37 ℃ after Yo 30min, with fluorescent microscope observation, sample uses observation of scanning report lens.

方法制作超高分子聚乙烯、钛合金和钴铬钼合金试样，表皮葡萄球菌经FITC标记，人工关节材料试样消毒后接种FITC标记的表皮葡萄球菌，试样表面分为光滑表面和粗糙表面，每组各6个试样，将含有细菌和试样的24孔板在37℃下孵育30min后，用荧光显微镜观察，试样用扫描电镜观察。

The antibacterial activities of most of fractions were similar to the pomegranate peel extract.(7) Respiratory inhibition experiment showed that the respiration of thallus was inhibited mainly through the hexose monophosphate pathway, which leaded to be short of NADPH, and induced metabolic function dysfunction. Growth curve assay showed that the pomegranate peel extract inhibited cytodieresis in logarithmic growth phase. After exposure to the pomegranate peel extract, bacterial protein was analyzed by SDS-PAGE. The results showed that protein synthesis was inhibited, especially for large molecular weight protein.

7呼吸抑制试验结果表明，石榴果皮提取物主要是通过抑制HMP途径，导致还原力NADPH缺乏，引起代谢功能障碍，达到抑菌的目的；加药组和正常组金黄色葡萄球菌生长曲线的对比结果表明，石榴果皮提取物能够抑制金黄色葡萄球菌对数生长期的菌体分裂；SDS-聚丙烯酰胺凝胶电泳分析表明，石榴果皮提取物能够抑制菌细胞蛋白合成，尤其对菌体内大分子量蛋白作用最明显。

The main pathogens of VAP were Gram negative organisms (68.63%) whose predominant pathogens were Pseudomonas aeruginosa and Acinetobacter. On the other hand, 75.40% of Gram positive organisms were Staphylococcus epidermidis.Of the pathogens 20.98% were eumycetes. Compared from June 1996 to June 2001 with from July 2001 to June 2006,there was no significant difference on the incidence rate of P.aeruginosa(31.76% vs 31.58%).During the recent 10 years,the incidence of Acinetobacter rose up from 8.23% to 15.23% and the rate of oxacillin-resistant Staphylococcus epidermidis rose up from 18.75% to 71.43%. The sensitivity of P.aeruginosa and Acinetobacter isolates descended to sodium imipenem cilastatin and ceftazidime in past 10 years.

在538例VAP中，2种及2种以上致病菌感染占56.51%；革兰阴性细菌中，铜绿假单胞菌和不动杆菌属为VAP的主要致病菌(68.63%)(385/561)；表皮葡萄球菌感染为VAP的主要革兰阳性致病菌(75.40%)(95/126)；真菌占20.98%(183/872)；铜绿假单胞菌在两个时间段所占比例无明显变化，不动杆菌属的检出率呈上升趋势(8.23%～15.23%)；铜绿假单胞菌和不动杆菌属对亚胺培南、头孢他啶的敏感性明显下降，耐苯唑西林表皮葡萄球菌的检出率显著升高(18.75%～71.43%)。

Objective To investigate the exfoliative toxin serotype genes and antibiotic resistance of Staphylococcus aureus isolated from children with staphylococcal scalded skin syndrome.

目的 探讨葡萄球菌性烫伤样皮肤综合征致病的金黄色葡萄球菌分泌表皮剥脱毒素的主要类型及耐药情况。

Exfoliative toxins of Staphylococcus aureus are directly responsible for the skin exfoliation of staphylococcal scalded skin syndrome.

由金黄色葡萄球菌分泌的表皮剥脱毒素是引起葡萄球菌性烫伤样皮肤综合征患者皮肤剥脱的直接原因。

Objective: To study the source of the separation of our hospitals to methicillin-resistant Staphylococcus aureus in molecular epidemiology of the characteristics, identify MRSA clinical isolates in our country, investigate the distribution characteristics of staphylococcal chromosomal cassette mec typing of MRSA and detect the Panton-Valentine leukocidin virulence gene of MRSA.

目的：研究我国医院来源分离到的耐甲氧西林金黄色葡萄球菌的分子流行病学特征，鉴定我国临床分离的MRSA，分析MRSA葡萄球菌染色体mec盒基因型，检测MRSA的PVL毒力基因。

In the in vivo experiment, 30 rabbits were divided into 3 of soft tissue on right proximate tibia in each rabbit, exposed the cavitas medullar of y the instillation of staphylococcus aureus suspension into wounds.

体外抑菌实验检测庆大霉素和活力碘对表皮葡萄球菌和金黄色葡萄球菌的最低抑菌浓度和最低杀菌浓度，药敏实验观察其分别对这两种细菌的抑菌圈大小。

Of the patients whose lesions were microbiologically evaluable, 96% achieved a clinical cure, including 95.7% of the patients with methicillin-susceptible S.

在病灶可进行微生物评价的患者中，96%达到了临床治愈，其中95.7%的患者存在对甲氧西林敏感的金黄色葡萄球菌感染，96.9%存在对甲氧西林耐药的金黄色葡萄球菌感染。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。