葡萄球菌

Haemolyticus had a closer relationship with ACME-arcA rather than with native arcA in Staphylococcus aureus and Staphylococcus epidermidis. A novel ccr allotype ccrAB_(SHP was identified in ACME-arcA-positive isolates.

ACME-arcA基因、arcA固有基因系统进化分析显示，MRSH的arcA固有基因和金黄色葡萄球菌、表皮葡萄球菌、溶血葡萄球菌的ACME-arcA基因的亲缘关系更近。

Results Among 1 439 strains of isolated bacteria,456(31.69% were gram-positive bacteria,and 206(45.18% of which were coagulase negative Staphylococcus epidermidis,92(20.18% were coagulase positive Staphylococcus aureus...

结果1 439株细菌中，革兰阳性菌456株(31.69%)，以凝固酶阴性表皮葡萄球菌为多，达206株(45.18%)，其次为金黄色葡萄球菌92株(20.18%)；两种葡萄球菌的耐药率相似，对万古霉素耐药率均为0。

Results In 585 staphylococcus, there were 406 staphylococcus aureus, which including 387 methicillin sensitive staphylococcus aureus and 19 methicillin resistant staphylococcus aureus. And there were 179 coagulase negative staphylococcus, including 20 methicillin sensitive coagulase negative staphylococcus and 159 methicillin resistant coagulase negative staphylococcus.

结果 585株葡萄球菌中，金黄色葡萄球菌406株，其中甲氧西林敏感的SA387株，甲氧西林耐药的SA19株；凝固酶阴性的葡萄球菌179株，其中甲氧西林敏感的CNS20株，甲氧西林耐药的CNS159株。

Results Among 70 strains of Staphylococcus spp., 56 strains were positive Staphylococcus coagulase gene with PCR; the same 56 strains were also positive with latex agglutination test; 54 of which were positive with tube test method, 2 strains were false negative; strong positive were detected with slide method, all these 56 strains were identified as Staphylococcus aureus by VITEK-Ⅱ automatic analyzer.

结果 70株葡萄球菌属细菌中，PCR法检测葡萄球菌凝固酶基因56株为阳性；Slidex Staph Plus与PCR法相对应的56株为阳性；试管法检测与前2种方法相对应的54株为阳性，2株为假阴性；玻片法为强阳性；VITEK-Ⅱ全自动分析仪鉴定56株为金黄色葡萄球菌

Methods S. epidermidis atlE gene expression level at different phases was detected by RT-PCR, and the accumulation of extracellular DNA at different phases was assessed by spectrophotometric measurements of light absorbance by DNA; DNase Ⅰ was used to study the function of extracellular DNA in biofilm formation and primary attachment. The effects of atlE gene deletion on initial adherent capacity, biofilm formation and extracellular DNA release were studied by construction of atlE deletion mutant via homologous recombination.

采用RT-PCR法检测表皮葡萄球菌atZE基因在不同时期的表达水平，用紫外分光光度计检测DNA的方法检测了相应时期的胞外DNA的释放量，并用DNA酶研究表皮葡萄球菌胞外DNA在生物膜形成和起始黏附中的作用；采用ρBT2质粒同源重组敲除的方法构建了表皮葡萄球菌1457的atlE基因突变株，研究atlE基因敲除突变对起始黏附能力、生物膜形成及胞外DNA释放能力的影响。

We have investigated the conformational features of four short peptide fragments of Staphylococcal nuclease by homonuclear two-dimensional NMR and circular dichroism with a view to examining the early events in the folding process of SNase.

中文题名金黄色葡萄球菌核酸酶折叠起始位点：N-末端短肽段的NMR研究副题名外文题名 Folding initiation sites of staphylococcal nuclease：exploring the solution conformation of N-terminal fragments by nuclear magnetic resonance 论文作者戴继勋导师王金凤研究员学科专业分子生物学研究领域\研究方向学位级别博士学位授予单位中国科学院生物物理研究所学位授予日期2001 论文页码总数68页关键词金黄色葡萄球菌核酸酶蛋白质折叠核磁共振馆藏号BSLW /2001 /Q71 /89 本论文工作用四个金黄色葡萄球菌核酸酶N-末端短片段作为新生肽链折叠的模型，用同核二维核磁共振谱和远紫外圆二色谱研究了它们的溶液构象特征。

The lipolytic and proteolytic activities of the 8 strains by the special Media were studied. It showed that Staphylococcus warneri, Staphylococcus saprophyticus and Lactobacillus plantarum had the proteolytic activity.

其中，松鼠葡萄球菌、木糖葡萄球菌和腐生葡萄球菌的耐盐性最强，在NaCl浓度为15％时，仍能生长；乳酸菌中，戊糖片球菌的耐盐性要高于植物乳杆菌。8株优势菌对亚硝酸盐均不敏感，在亚硝酸钠浓度为0.15％时，生长良好。

Lactobacillus plantarum had lipolytic activity. Bacillus subtilis not only had the lipolysis but also had proteolysis.Single strains fermenting experiment showed that, the effect of different strain to the pH, acidity, the total amino acid were not signifient, Pediococcus pentosaceusand Lactobacillus plantarum could improve the content of free amino acid in dried beef.

研究结果表明：沃氏葡萄球菌、腐生葡萄球菌和植物乳杆菌只有脂肪酶活性，无蛋白酶活性；松鼠葡萄球具有弱蛋白酶活性；木糖葡萄球菌具有弱脂肪酶活性；戊糖片球菌具有脂肪酶活性，而蛋白酶活性较弱；枯草芽孢杆菌具有两种酶活性。

The minimal bactericidal concentration of PTKE on Escherichia coli, Staphylococcus aureus, Salmonella, Pasteurella multocida and Streptococcus agalactiae were 100, 25, 50, 25 and 50 mg/mL respectively. The isolation of EA, EB and EC had no antibacterial effect on Escherichia coli and Pasteurella multocida. MBC of EA on Staphylococcus aureus and Salmonella were 2.5 and 5 mg/mL, and MBC of EB on Staphylococcus aureus, Salmonella and Streptococcus agalactiae were 1.25, 5 and 2.5 mg/mL respectively, while MBC of EC on Staphylococcus aureus was 2.5 mg/mL. Polygonum taibaishanense Kung.

PTKE对大肠埃希菌、金黄色葡萄球菌、沙门氏菌、多杀性巴氏杆菌和无乳链球菌的最低杀菌浓度分别为100，25，50，25和50 mg/mL；而PTKE的分离物EA、EB和EC对大肠埃希菌和巴氏杆菌无抑菌作用，EA对金黄色葡萄球菌、沙门氏菌的MBC分别为2.5和5.0 mg/mL，EB对金黄色葡萄球菌、沙门氏菌和无乳链球菌的MBC分别为1.25，5.0和2.5 mg/mL，EC对金黄色葡萄球菌的MBC为2.5 mg/mL。

objective to study the effects of rabdosiae serrae compound preparation on staphylococcus aureus biofilm.methods minimal inhibitory coh centration were tested by doubling dilution,and simulate s.aureus biofilm in vitro be improved brown plate method.the effects of r.serrae compound preparation in different mic on s.aureus biofilm were determined and confirmed by scanning electronic microscope.single layer cells of human floss film were used to test the toxic effects of the compoud preparation.results the mic of s.serrae compound to s.aureus was 128?

目的 研究溪黄草复方对金黄色葡萄球菌生物膜的影响。方法采用试管二倍稀释法测定药物的最低抑菌浓度，用brown平板改良法体外复制金黄色葡萄球菌生物膜模型，并检测溪黄草复方的不同mic浓度对金黄色葡萄球菌生物膜的影响，扫描电镜确认。采用人绒毛膜单层细胞体外培养检测受试药物的毒性作用。结果溪黄草复方对金黄色葡萄球菌的mic值为128？

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。