葡萄球菌

Of Staphylococcus aureus was methicillin-resistant Staphylococcus aureus and 77.35% of coagulase negative Staphylococcus was methicillin-resistant coagulase negative Staphylococcus respectively.

耐甲氧西林金黄色葡萄球菌和耐甲氧西林凝固酶阴性葡萄球菌分别占金黄色葡萄球菌和凝固酶阴性葡萄球菌的60.58%和77.35%。

Results Ganmaofang had definite bacteriostatic action on standard strain of staphylococcus aureus and escherichia coli, and clinical dissociative strain of staphylococcus aureus, escherichia coli, klebsislla pneumoniae, Staphylococcus epidermidis, Staphylococcus intermedius, Staphylococcus simulans and beta streptococcus in vitro. Its MIC is between 0.075 g/mL and 0.2 g/mL. Ganmaofang could prolong the survival time of mouse infected by staphylococcus aureus and decrease the mortality of ill mouse, meanwhile it could enhance the survival rate of mouse infected by pneumoniae diplococcus.

结果感冒方体外对标准菌株金黄色葡萄球菌、大肠杆菌和临床分离菌株金黄色葡萄球菌、大肠杆菌、肺炎克雷伯菌、表皮葡萄球菌、中葡萄球菌、溶葡萄球菌、乙型链球菌具有一定的抑菌作用，MIC 0.075~0.2 g/mL，体内可延长金黄色葡萄球菌感染小鼠的存活时间和减少感染小鼠的病死率，对肺炎双球菌感染小鼠可提高感染小鼠的存活率。

Besivance is indicated for the treatment of bacterial conjunctivitis in patients aged ≥1 year whose infection is caused by susceptible isolates of the following bacteria: CDC coryneform group G, Corynebacterium pseudodiphtheriticum, Corynebacterium striatum, Haemophilus influenzae, Moraxella lacunata, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus lugdunensis, Streptococcus mitis group, Streptococcus oralis, Streptococcus pneumoniae, and Streptococcus salivarius.

Besivance适用于治疗年龄≥1 岁、由下列细菌的致病分离物所致的细菌性结膜炎患者：CDC棒状杆菌G群，假白喉棒杆菌，纹带棒杆菌，流感嗜血杆菌，腔隙莫拉菌，金黄色葡萄球菌，表皮葡萄球菌，人葡萄球菌，路邓葡萄球菌，缓症链球菌群，口腔链球菌，肺炎链球菌以及唾液链球菌。

Hematolytic staphylococcus, staphylococcus epiderrnidis, torpidity staphylococcus, squirrel staphylococcus and mimicry staphylococcus were the top five pathogenic bacteria.

结果 在所进行的8220次细菌学检测中共发现1320株阳性细菌，其中发现202株CNS，占总体检出率的15.3％（202／1320），其中溶血葡萄球菌、表皮葡萄球菌、缓慢葡萄球菌、松鼠葡萄球菌、模仿葡萄球菌等为主要的前5位致病菌。

There were 70 strains isolated from each of the MRS and VJ at the different stage of the processing of dried beef. Staphylococcus warneri, Staphylococcus sciuri, Staphylococcus auricularis, Bacillus subtilis, Staphylococcus xylosus, Staphylococcus saprophyticus, Lactobacillus plantarum and Pediococcus pentosaceus were 8 predominate bacteria. The growth curve of predominate bacteria was studied. The result showed that growth of Pediococcus pentosaceus was faster than that of Lactobacillus plantarum; the growth of Staphylococcus sciuri is fastest and Staphylococcus auricularis was slowest.

在牛肉自然发酵加工过程中的不同阶段，利用MRS和PCA~+培养基分离到乳酸菌和微球菌各70株，经过分析鉴定，得到8株优势菌，分别为沃氏葡萄球菌、松鼠葡萄球菌、耳氏葡萄球菌、木糖葡萄球菌、腐生葡萄球菌、枯草芽孢杆菌、植物乳杆菌和戊糖片球菌。

Staphylococcus auricularis, Staphylococcus xylosus and Pediococcus pentosaceus had a signifient effect to the sensory quality of dried beef, Pediococcus. pentosaceu had a most signifiennt effect to improve dried beefs quality. According to all the factors, Staphylococcus xylosus, Lactobacillus plantarum and Pediococcus pentosaceus were proved to be appropriate.

结果表明：各菌株对发酵牛肉成品的pH、酸度、氨基酸总量的影响未达到显著水平；植物乳杆菌、戊糖片球菌具有增加产品中游离脂肪酸含量作用；松鼠葡萄球菌、耳氏葡萄球菌、枯草芽孢杆菌、木糖葡萄球菌、腐生葡萄球菌和戊糖片球菌则具有显著增加不同游离氨基酸的作用；从感官评价结果来看，耳氏葡萄球菌、木糖葡萄球菌、戊糖片球菌能显著影响产品的感官特性，其中戊糖片球菌能显著提高产品感官评价分值。

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

objective to analyze the drug resistance of staphylococcus aureusand provide suggestion for clinical rational use of antibiotics.methods secretion of those neonates suffered primary skin soft tissue infection and admitted during june,2005 to december,2007 was cultured and 113 strains of staphylococcus aureus were detected.we used k-b diffusion methods to determine their susceptibility to the common used antibiotics.results resistance rates of staphylococcus aureus to ampicillin/sulbactam,penicillin-g and cefazolin were 14.2%,40.7%and 6.2% respectively; there was no vancomycin-resistant strain.conclusion the resistance of staphylococcus aureus is not a serious problem in neonatal dermatic soft tissue infection.local treatment is the major approach in clinic,while general anti-infection consisting mainly of penicillin and first generation cephalosporin is chosen to avoid resistance mutation of staphylococcus aureus resulted from abuse of antibiotics.

多重聚合酶链反应快速鉴别葡萄球菌及。。。乳腺脓肿分离到葡萄球菌的鉴定和药敏。。。目的探讨金黄色葡萄球菌的耐药性，指导临床合理用药。方法对我院2005年6月至2007年12月新生儿原发性皮肤软组织感染患儿的分泌物进行培养，共检出金黄色葡萄球菌113株，并采用kb纸片扩散法对其进行常用抗生素的药敏试验。结果金黄色葡萄球菌对氨苄西林/舒巴坦、青霉素g及头孢唑啉的耐药率分别为14.2%、40.7%和6.2%，未发现耐万古霉素金黄色葡萄球菌。结论在新生儿皮肤软组织感染中金黄色葡萄球菌的耐药问题并不十分严重，临床处理应以局部为主，全身抗感染治疗宜以青霉素及i代头孢类抗生素为主，避免过度使用抗生素加速金葡菌的耐药突变。

Some retrospective studies have proved that the clinical situations of Panton-Valentine leukocidin一producing Staphylococcus aureus infections are distinctive,especially necrotizing pneumonia caused by PVL-positive S aureus seems to be epidemic CA—MRSA stains causing skin and soft tissue infections,necrotizing pneumonia and lethal necrotizing fasciitis.

呼吸系统论文：近年来，国外对葡萄球菌杀白细胞素进行了大量的研究，多数研究显示产金黄色葡萄球菌感染有着特有的临床表现，特别是产PVL金黄色葡萄球菌相关性坏死性肺炎可能为一新现的临床疾病，由此PVL在金黄色葡萄球菌感染中的致病研究日益受到重视。

objective to study the infection and sensitivity to antimicrobial agents of staphylococcus of bacterial prostatitis patients.methods the results of bacteriology and drug susceptibility of 421 patients,who were suffered from prostatitis,were analysed retrospectively.results in the 421 succus prostaticus samples,278 strains pathogenic microorganisms of 15 genera were isolated from the 273 succus prostaticus samples,and the major pathogenic microorganisms was staphylococcus aureus(57.2%) and coagulase negative staphylocous（17.3%.the drug resistance rate of staphylococcus to penicilling,erythromycin and albomycin was 88%-97%,and it was 33%-50% to fluoroquinolones.the drug resistance rate of staphylococcus to amikacin,cefazolin,nemycin,rifampicin and cefoperazone was lower than 30%,and they all appeared susceptible to vancomycin.conclusion staphylococcus is the main germ of bacterial prostatitis and it appears high drug resistance to usual antibacterials.staphylococcus is susceptible to vancomycin,amikacin,cefazolin,neomycin,rifampicin and cefoperazone.

目的 探讨细菌性前列腺炎患者葡萄球菌的感染状况及抗菌药物敏感性。方法回顾分析421例前列腺炎患者标本的细菌学和药物敏感性试验结果。结果 421例前列腺液标本，273例分离出细菌，共分离出15个种别共计278株病原菌，其中金黄色葡萄球菌占57.2%，凝固酶阴性葡萄球菌占17.3%；葡萄球菌对青霉素g、红霉素、白霉素耐药率为88%～97%，对氟喹诺酮类耐药率为33%～50%，对丁胺卡那霉素、头孢唑啉、新霉素、利福平和头孢哌酮耐药率均0%，对万古霉素全部显示敏感。结论葡萄球菌是细菌性前列腺炎的最常见病原菌，对临床常用抗菌药物具有较高的耐药性，葡萄球菌对万古霉素、丁胺卡那霉素、头孢唑啉、新霉素、利福平和头孢哌酮较为敏感。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。