葡糖苷酶

Objective:To study the changes of neutral α-glucoside activity in the epididymis of heroin-dependent and heroin-withdrawal rats,and to investigate the effects of intervention with purine nucleotide.

目的：研究海洛因依赖及戒断大鼠附睾中性α葡糖苷酶含量的变化，及嘌呤核苷酸干预的影响。

The result showed that twocDNA fragments which expressed at high level both in shoot and radicle representedthe gene encoding beta-D-glucosidase; one cDNA fragment expressed specifically inshoot represented the gene encoding mitochondria HSP60; most clones of MF12 andMF17 fragments respectively represented the chloroplast genes encoding prp22 andprp19 proteins which are two components of ribosomal small subunit; while thededuced amino acid sequence from each exceptional one clone was respectivelyhomologous to CDC5 proteins and vesicle-associated membrane proteins; otherthree cDNA fragments expressed preferentially in shoot had no homologue inGenBank.

结果发现2个在胚芽和胚根中表达量都很高的cDNA片段代表的是编码玉米β-D-葡糖苷酶的基因；一个在胚芽中表达而在胚根中不表达的片段代表的是编码线粒体分子伴侣HSP60的基因；片段MFl2的大部分克隆测序结果是与叶绿体基因组中编码核糖体小亚基蛋白prp22的基因同源，但其中有一个克隆测定的cDNA片段序列推测的氨基酸序列与CDC5家族成员有较高的同源性；片段MF17的大部分克隆测序结果与叶绿体基因组中编码核糖体蛋白prp19的基因同源，而有一克隆测定的cDNA片段序列推测的氨基酸序列与参与信号转导的膜结合蛋白VAP27和VAMP有较高的同源性；另有3个优先在胚芽中表达的cDNA片段未查询到同源序列。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

Glucuronidase and lysozyme release was quantitated by enzyme reactions in which phenolphthaleinglucuronic acid and Micrococcus Lysodeikticus were as the substrates, respectively.

以酚酞葡糖醛酸和Micrococcus Lysodeikticus为基质的酶反应法测定β-葡糖苷酸酶和溶菌酶的释放量。

Compound 3 showed a significant inhibitory effect on release of β-glucuronidase rat polymorphous nuclear leukocytes activated by platelet activating factor.

另外，化合物3对PAF刺激的小鼠多形核白细胞β-葡糖苷酸酶的释放具有明显的抑制作用。

We conducted a randomized, placebo-controlled trial of alglucosidase alfa, a recombinant human GAA, for the treatment of late-onset Pompe''s disease.

咱们进行了一项用阿葡糖苷酶α治疗晚发式蓬佩病的RAND、慰藉剂对于照试验。

The recent development on the research of the plant myrosinase/glucosinolate system are reviewed, including the organization of myrosinase gene family, their origin, evolution, and the potential functions of the system.

对植物芥子酶防御系统研究进展进行了综述，包括基因家族的结构、基因的表达调控、芥子酶的细胞定位、植物以外其它生物的芥子酶、硫代葡糖苷／芥子酶系统起源进化以及其可能功能等。

After inoculation of leukemia virus,the changes in ANAE,AcP and β-Gase reactions were observed in the thymocyte and thymie nurse cell in thymus cortex of experimental mice.

对接种白血病病毒后30d、75d 小鼠胸腺和淋巴结的组织结构及酸性非特异性酯酶、酸性磷酸酶、β～葡糖苷酸酶反应类型进行了观察，并与正常组作对照。

The esterification reaction kinetics of hexanoic acid with butanol, catalyzed by Novozym 435 lipase, was studied in water-in-oil microemulsions formed by alkyl polyglucoside C10G1.54/n-butanollcyclohexane/water buffer solution.

研究了Novozym435脂肪酶在烷基聚葡糖苷形成的W/O微乳液体系中，催化正丁醇与正己酸合成酯的反应动力学。

The esterification reaction and itsinfluencing factors of hexanoic acid with n-butanol, catalyzed by lipaseNovozym100T, were studied in water-in-oil microemulsions formed by alkylpolyglucoside C_(12)G_(1.50)/n-butanol/cyclohexane/water system.

研究发现，十二烷基聚葡糖苷C_(12G_(1.50)／正丁醇／环己烷／H_2O体系形成的油包水微乳液中，脂肪酶Novozym100T催化正己酸和正丁醇的酯合成反应，反应初始速率V_0随正己酸的初始浓度C_~0增大而增大；随正丁醇的初始浓度C_~0的增大而减小，后V_0进入一&坪区&。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。