萤火虫

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus， BFV)中国株BFV3026原病毒DNA为材料，构建R区系列缺失质粒，通过对其转染细胞中RT水平及对缺失质粒与luc报告质粒共转染细胞中萤火虫荧光素酶活性的测定，确立U5区对于BFV3026两类启动子LTR和IP均具有负调控作用；同时将带有不同R区的BFV3026结构基因片段克隆于异源启动子CMV之下，通过对其转染细胞293T中RT酶活性的测定，确立R区对于病毒结构基因pol的表达具有一定的调节作用，并将其功能区域初步界定在R区5′端100bp内。

In this recombinant virus the Luc gene was under dual conntrol of the synthetic and XIV promotors.

在该重组杆状病毒株，萤火虫荧光素酶基因受到合成启动子和XIV启动子的双重调控。

We then hurriedly assembled at the shed for watching fireflies at the reserve in the village.

随后，我们很快在小屋处集合，按村里的安排观赏萤火虫。

The red len produces discrete lighting to preserve night vision for star-gazing, firefly-watching and changing baby's diapers.

红色色片提供单一红色色光让观星者、赏萤火虫人员及夜间更换小孩尿布者眼睛保有夜视能力。

He would see flashes like a swarm of fire-flies floating before his eyes, or a sea of deep mist around him, with luminous waves of molten silver.

他看见像一群火般的闪电――萤火虫在他眼前漂浮，或者大量的薄雾围绕着他，带有熔解的银子那样的发光波浪。

I will become a firely and even in the day my glow will be seen in spite of the sun.

我愿成为一只萤火虫，即使是在白天，不管阳光多么耀眼，我的光芒也将无法阻挡。

I will become a firefly and even in the day my glow will be seen in spite of the sun.

从此我要记住萤火虫的启迪：只有在振翅的时候，才能发出光芒。

I will become a firefly and even in the day my glow will be seen in spite of the sun.

从此我要记住萤火虫的启迪：只有振翅的时候，才能发出光芒。

I will become a firefly and even in the day my glow will be seen in spite of the sun.let others be as butterflies who preen their wings yet depend on the charity of a flower for life.

我要成为一只萤火虫，即使艳阳高照，也无法掩盖我的光芒。

Evading a massive texas Rangers dragnet as well as a group of equally murderous bounty hunters led by Ken Dwyer the brother of a policeman Mamma Firefly killed in \\\'House of...

回避了一个庞大的德州游骑兵拖网以及同样凶残的赏金由Ken德怀尔领导（一名警察妈妈萤火虫猎人团体杀害的弟弟在\ \ \'的。。。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。