萤光素

The tiny insects glow when the naturally-produced luciferase comes in contact with adenosine triphosphate, an energy source for all living things.

这种小型的昆虫会在自然生产的萤光酵素和三磷酸腺●（ATP，是所有生物的能量来源）接触时发光。

Flavin: Any of a class of organic compounds, pale yellow biological pigment s that fluoresce green.

黄素：一组浅黄、绿萤光生物色素，在植物和动物组织中分布很广，含量少。

Flavin: Any of a class of organic compounds, pale yellow biological pigments that fluoresce green.

黄素：一组浅黄、绿萤光生物色素，在植物和动物组织中分布很广，含量少。

In this thesis, we did modifications of alkyl groups upon benzyl fluorophosphonate. In order to study the influence of alkyl length and steric effect on activity and selectivity of probes, we synthesized benzyl fluorophosphonate recognition heads with modifications including ethyl, butyl, octyl and cyclohexyl. By way of connecting different kinds of tag like azido group, biotin and rhodamine with recognition heads separately through a higher hydrophilic ethylene glycol derived linker, a series of benzyl fluorophosphonate probes were made up. And they will apply to various biological testing strategies.

在本论文中，我们以苯甲基氟化磷酸酯为基础，於其上进行一系列烷基之修饰，为了探讨烷基长度与空间立体性质对於探针活性及选择性的影响，我们合成了包含乙烷基、丁烷基、辛烷基以及环己烷基修饰的苯甲基氟化磷酸酯辨识端，并藉由具较高亲水性的聚乙二醇衍生连接桥结合三种不同的发报端，分别有叠氮基团、生物素及萤光基团来构成不同的化学探针组合，这一系列苯甲基氟化磷酸酯类化学探针将可被应用於不同的生物测试策略。

Double fluorescent immunohistochemical staining revealed close apposition of VIP-, PACAP-, and vasopressin-immunoreactive terminals with tyrosine hydroxylase-ir neurons within the dorsomedial arcuate nucleus. We were then of interest to learn from where VIP-, PACAP- and AVP-containing neurons that project to the ARN originate.

经由双重免疫萤光染色法，可以在弓状核的背中区，具酪胺酸羟化酶免疫活性的细胞旁，发现有VIP、PACAP及血管加压素免疫活性的神经末稍的分布及并置，显示确有VIP，PACAP及AVP之神经元投射至弓状核。

We synthesized a new long-wavelength latent fluorogenic probe BQC (1) to monitor DT Diaphorase activity.

设计合成对於还原酵素具有专一作用之新隐藏型长波长萤光探针BQC。

Can reverse JA-induced migration inhibition JA-treated cells also showed significantly higher adhesion to laminin In conclusion these results indicate a novel anti-tumor role of JA via reducing reorganization of cytoskeleton increasing integrin 3 expression and adhesion ability to inhibit tumor cells migration Furthermore immunofluorescence staining of phalloidin/actin indicated that JA-treated cells exhibited cytoskeleton rearrangement and significant decreases in the number and length of filopodia Activity of Rho-family small GTPases was detected by a GST-pull down assay and showed that GTP-loaded Cdc42 and Rac were decreased in JA-treated cells In the future we hope these findings will be helpful to further understand the mechanisms underlying tumor progression in oral cancer and subsequently improve the cancer prevention and treatment

以抗体阻断integrin ？3可以有效使OC2恢复被JA所抑制的迁移能力。细胞贴附实验指出JA能提高OC-2在laminin环境下的贴附能力。当我们进一步以免疫萤光染色观察细胞，发现在JA处理下的OC-2，其外形、细胞骨架和细胞边缘的filopodia的密度和长度明显减少，利用GST-pull down assay侦测到Cdc42-GTP和Rac-GTP的表现量降低。推论爵床素A可能透过刺激口腔癌细胞大量表现integrin ？3，使细胞贴附能力增加或是透过改变细胞骨架来降低细胞迁移能力。希望本研究对於JA所做的抗癌及抗转移效应评估能提供口腔癌治疗在研发上一些帮助。

In the first part of this thesis, a continuously flouremetric assay for PAPS synthetase was developed for the first time. It was performed by coupling with the physiological reaction of phenol sulfotransferase.

本论文主要分为两部份，第一部份主要探讨的是 PAPSS萤光检测法，此活性检测法的操作结合了酚亚硫酸转移酵素(phenol sulfotransferase， PST)的生理活性，为一灵敏度极高的活性检测法。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐