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QN 〓PHAL,〓PHAL and 〓PHAL were quaternized by refluxing with benzyl bromide to give ligands 8, 9 and 10 respectively. 2. The chiral ligands were applied to the AD reaction of olefins with NMO as co-oxidant and PEG (400 MW) or PF〓 as reaction media.

以奎宁和奎尼定为原料分别与1,4-二氯-2,3-二氮杂萘反应制备〓PHAL和〓PHAL,然后通过〓PHAL、〓PHAL及〓PHAL与苄基溴的季铵化反应制备单季铵盐手性配体8、9和10。

The results indicated that phenol, methylphenols and naphthols, with high concentration, exsisted in coking wastewater, and a small quantity of chorophenols and nitrophenols was also inside it.

实验结果表明,焦化废水中含有高浓度的苯酚、甲基酚和萘酚,同时存在微量的氯酚和硝基酚。

In this paper,plumules,cotyledons,hypocotyls,leaf blades,petioles and stalks of physic nut (Jatropha curcas L.) were used as explants,and callus induction and plant regeneration were studied on MS medium contained different concentrations of 6-BA and NAA.

以小桐子的胚芽、子叶、下胚轴、叶柄、叶片和茎段作为外植体,用不同浓度的6-苄基腺嘌呤(6-BA)和α-萘乙酸对其进行愈伤组织的诱导和植株再生的研究。

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明,当变性液中盐酸胍浓度约为1.0 mol/L时,芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体,其去折叠过程符合&三态模型&;荧光探针结果表明,在溶液中盐酸胍浓度约为1.0 mol/L时,中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域;荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况,结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个;蛋白电泳和体积排阻色谱结果表明,在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中,不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

Sodium malate and 1-Naphthyl phosphoric acid sodium were used as substrates. NBT and fast blue RR salt were used to stain MDH and ALP respectively.

鄄萘磷酸钠盐为特异性底物,氯化硝基四氮唑蓝和固蓝RR盐为染色剂,分别对MDH和ALP染色,以相对迁移率标记酶带。

In this dissertation, a kind of MPc and five metallonaphthalocyanine were synthesized. The molecular structure of the compounds were characterized by means of the elemental analysis, the infrared spectrum and the nuclear magnetic resonance spectrum, which proved that the topical compounds were successfully synthesized.

本文设计并合成了五种叔丁基取代的金属萘酞菁化合物和一种金属酞菁化合物,通过元素分析、红外光谱分析、核磁共振氢谱分析等对目标产物进行了结构表征,验证了所合成化合物的结构。

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