菌膜

As a result, there were many cicatrice sculptured in faces of PET film, which were degraded with TA as the common metabolism, after one month.

通过电子显微镜观察降解后膜的表面，结果表明：通过长期驯化的F4和p.ch菌株，都能使PET膜表面产生明显刻蚀孔洞；且在富氧的条件下，以TA为共代谢基质降解PET膜，在一个月内能将PET膜表面刻蚀出较大空洞。

Our main high-tech products are the Germs Collector, Single-use sterile closed canister, Reusable Filter, Sterility Isolation System, Negative Pressure Isolator, TOC Analyzer, Dissolution Tester, VHP Sterilizer, Foreign Matter Detector, Aperture Measuring Apparatus, Shaker, Homogenizer, Temperature Chamber and Microorganism Limit Testing System and so on.

公司主营产品有集菌培养器系列、集菌仪系列、无菌隔离系统、负压隔离器、水中总有机碳分析仪、药物溶出仪、汽化过氧化氢灭菌器、可见异物检测仪、微孔滤膜孔径测定仪、无菌检查专用振荡仪、微生物限度检验仪等一系列高新技术产品。

And this strain degraded H〓S without lag time. The heterotrophic bacteria's generation period was short and its growth was rapid. The result of experiment indicated when the strain Hm-6 was applying to the packed tower, the startup was more quickly and the control was more convenient than the autotrophic bacterium. Applying to deodorize the H〓S, the better removalability could be got. At the same time, comparing with the system used at home of which the biofilm directly formed with activated sludge, it runs in the neutral condition, has lower corrusivity to the equipment and was liable to handle. And this system didn't need the acclimation to become biofilm, so it can save a rather long acclimation time.

异养菌虽然不以硫化物作为能源物质，但其世代周期短、生长迅速，运行管理比自养菌更为方便，具有周期短，启动迅速等优点，接种了菌株Hm-6泥炭生物填料塔，对低浓度H〓S有较高的去除效果，且没有延迟期，与国内通常采用的直接以活性污泥挂膜的方式相比，应用该菌的脱臭系统在中性条件下运行，对设备的腐蚀性较低、运行过程较易控制，具有很好的应用前景。

In the current study, a series of cationic α-helical peptides of 20 a.a. were designed and synthesized based on four structural parameters, including charge, polar angle, hydrophobicity, and hydrophobic moment. Several methods were performed to describe the influence of these parameters on the antimicrobial and hemolytic activity, and on the permeabilizing effect on model membranes. The potential of these parameters to increase the antibacterial activity and to improve the prokaryotic selectivity is assessed.

本实验利用自行设计合成的人工抗菌胜肽当模型，固定长度为 20 个胺基酸，改变其电荷、极性角度、疏水性及疏水性矩等四种结构特性，并利用其对各种不同病原菌之抗菌活性及对兔子红血球细胞的溶血活性来研究其结构特性的改变对其抗菌活性、选择性及微脂体膜的渗透性之影响，以期望改变结构参数能增加抗菌活性及对原核的选择性。

A kind of virus particles were observed in cytoplasm of sampled ill sea cucumber tissues which were sampled from sick sea cucumbers with ulcerous skin and swollen mouth from their farming ponds at the beginning of 2005. The particles were covered with a bilayer envelops, shaped in globular with its size 100-200 nm in diameter. The same virus was isolated from cultured sea cucumbers with disease from different time and sites, and then infected the healthy sea cucumber. sea cucumber appeared the phenomenon such as ulcerous skin and swollen mouth and the same virus was isolated from infected sea cucumbers.

本章从患病刺参中分离出了2株对刺参具有感染能力的细菌，经鉴定1株为弧菌科弧菌属Vibrio sp和1株为弧菌科施万氏菌属Shewanella sp；在患病刺参的触手、肠和呼吸树等组织的细胞质中，检出了球形、具双层囊膜、直径大约为100-200nm的病毒粒子利用分离的病毒进行人工感染实验，刺参可以产生与自然发病刺参相同的症状，显示了分离的病毒对刺参具有一定的感染性。

Significant loss of GEMs was not observed but strong growth activities, formation of biofilm and zoogloea on the surface of immobile particles were observed via scanning electron microscope. The mechanical stability of the particles remained unchanged after nearly two-month operation.

在整个反应过程中，没有出现大量工程菌流失的现象，同时在固定化颗粒的表面以及浅层均观察到了大量工程菌菌体，固定化颗粒的表面还出现了生物膜和菌胶团，反应结束时，颗粒形态完好，强度满足本工艺条件下长期使用的需求。

According to the above study result, the diversity of microorganisms population in the SUFR system is complex and the bio-community formed in the SUFR system is stable. The close relations between abundance of bacteria and nutrients were found. The correlation between heterotrophic bacteria and COD was 0.949. The correlation between organic phosphate bacteria and TP was 0.815. The correlation between nitrosobacteria and NH3-N was 0.909. The correlation between disnitrifier bacteria and TN was 0.653. These inneglectable great factors effecting on phosphorus-uptake and phosphorus-release of phosphorus accumulating organisms are the influent COD、the concentration of DO、the sludge retention time、the temperature. The diversity of organic phosphate bacteria population in the SUFR reactor was complex and there is no phosphate bacteria taking the absolute superiority in quantity. The near-native pure culture method powerfully complement the traditional pure culture technique and enrich culture technique. The sludgy bioactivity is very good in whole SUFR reactor. By the quality of being biochemistry, the anaerobic phase is higher than anoxic phase and the anoxic phase is higher than aerobic phase of SUFR.

通过以上试验内容研究发现，SUFR 系统中的微型动物种群较多，微生物生态系统稳定；系统中微生物的数量与营养盐的含量密切相关，其中总异养菌与COD的相关系数r 为0.949，有机磷细菌与TP 的相关系数r 为0.815，亚硝化菌与NH4+-N的相关系数r 为0.909，反硝化菌的数量和TN 的相关系数r 为0.653；污水生物除磷工艺中进水COD 的浓度、DO 浓度、泥龄的长短、温度都是影响聚磷菌释磷及吸磷效果的不可忽视的因素；SUFR 系统中的磷细菌呈现种群多样化的趋势，没有占绝对优势数量的磷细菌；微孔滤膜近自然培养法是对传统纯培养技术和富集培养技术的有力补充；整个SUFR 反应器系统中污泥的生物活性很好，就可生化性来讲，厌氧反应器大于缺氧反应器大于好氧反应器；按照动力学方程式求出的细胞生长动力学特征值和SUFR 脱氮除磷系统工艺的实测值结果基本吻合。

objective to study the effects of rabdosiae serrae compound preparation on staphylococcus aureus biofilm.methods minimal inhibitory coh centration were tested by doubling dilution,and simulate s.aureus biofilm in vitro be improved brown plate method.the effects of r.serrae compound preparation in different mic on s.aureus biofilm were determined and confirmed by scanning electronic microscope.single layer cells of human floss film were used to test the toxic effects of the compoud preparation.results the mic of s.serrae compound to s.aureus was 128?

目的 研究溪黄草复方对金黄色葡萄球菌生物膜的影响。方法采用试管二倍稀释法测定药物的最低抑菌浓度，用brown平板改良法体外复制金黄色葡萄球菌生物膜模型，并检测溪黄草复方的不同mic浓度对金黄色葡萄球菌生物膜的影响，扫描电镜确认。采用人绒毛膜单层细胞体外培养检测受试药物的毒性作用。结果溪黄草复方对金黄色葡萄球菌的mic值为128？

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

The study on treatment of waste water of straw alkali pulp by white rot fungi has been made systemically in laboratory, the optimization conditions of white rot fungi culture have been given, and the relevant pilot scale equipment of white rot fungi treatment has been setup first in China.

在完成了白腐菌培养的优化条件，系统进行了白腐菌处理碱法草浆含氯漂白废水的实验室试验的基础上，进入工厂废水处理现场，自行设计安装了一套以不锈钢丝为载体，用白腐菌挂膜的生物接触中试反应器，在国内建立了第一个白腐菌处理碱法草浆废液的工厂现场中试基地。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。