菌膜

The major direction of the research within the first year will be outlined as following:( 1 )The background study, collection, analysis, and identification of the possible metabolites such as protease, hemolysin, hemagglutinin, cytotoxin, leukocidin, and lipopolysaccharide;( 2 )The collection of the pathogenic factor such as outer membrane protein of vibrio spp.;( 3 )The analysis of pathogenic factor through the application of SDS-PAGE technique; and ( 4 )The activity assay of outer membrane protein on substrate to characterize the possible function or study the reaction mechanism.

致病因子之收集：将利用生物化学之方法，以弧菌属细菌外膜蛋白质为目标，收集与溶血素相关之致病因子。3。致病因子之分析：将利用SDS-PAGE电泳，对所收集得到之致病因子进行分析，并估算其可能之分子量。4。致病因子之活性测试：将利用所纯化之外膜蛋白质进行对受质活性之测试，以确定其功能或可能之作用机制。

The oxidase system includes both membrane-bound and soluble cytosolic proteins. The primary feature of this enzyme is stimulus-dependent activation and which mechanism is the phosphorylation and translocation of a complex of cytosolic factors to the membrane, where they associate with a flavocytochrome enzyme.The fully assembled complex functions as an electron transport system,moving electrons from cytosolic NADPH to molecular oxygen to form superoxide anion（O2-）, which, along with subsequent reactive products called respiratory burst,exerts microbicidal and cytotoxic activities.

此氧化酶体系包括膜结合的和可溶的胞浆蛋白，其主要特征为依赖刺激而活化，机制为复合体的胞浆因子磷酸化并转位至膜，在这里与细胞色素酶相关联，组装完全的复合体在功能上作为一个电子转运体系，从胞浆NADPH转移电子给分子氧形成超氧阴离子（O2-），伴随着接下来的活性物质产生，也叫呼吸爆发，发挥抗菌和抗细胞毒素的活性。

Long running performance showed that due to the membrane interception,the nitrobacter is enriched in reactor in the interest of improving the nitrification rate;the maximum ammonia nitrogen loading can be 0.19 kg/(m3·d) with effluent ammonia nitrogen＜1 mg/L (removal rate 99%).

采用浸没式膜生物反应器处理焦化废水的试验结果表明：膜的截留作用可使硝化菌在反应器内富集而有利于提高系统的硝化能力，其去除氨氮的最高负荷为0.19kg/(m3·d)，出水氨氮＜1mg/L(去除率为99%)；泥龄长可能使微生物的代谢产物或其他大分子物质积累，从而

The characters and performance of the biofilm were studied. When the concentration of nitrite is 206.82 mg/L, the velocity of nitrification is 260 mg/L·d. The best performed bacteria, N-20, were identified as Nitrobacter sp.

实验得到了稳定的自养硝化生物膜；当NO-2浓度为206.82mg/L时，生物膜的稳定硝化速率可达260mg/L·d；经鉴定，硝化速率最高的N20菌株属硝化杆菌属(Nitrobactersp。)。

A submerged membrane sequencing batch bioreactor was used to treat coke wastewater.Long running performance showed that due to the membrane interception,the nitrobacter is enriched in reactor in the interest of improving the nitrification rate;the maximum ammonia nitrogen loading can be 0.19 kg/(m3·d) with effluent ammonia nitrogen＜1 mg/L (removal rate 99%).Long sludge retention time may result in the accumulation of metabolic products and high molecular materials,and thus inhibiting activity of nitrate bacteria and causing a ccumulation of NO2-,which is beneficial to the running of short-cut denitrif ication.However,too long retention time will affect the activity of nitrite bacteria,detrimental to the treatment effect of ammonia nitrogen.

采用浸没式膜生物反应器处理焦化废水的试验结果表明：膜的截留作用可使硝化菌在反应器内富集而有利于提高系统的硝化能力，其去除氨氮的最高负荷为0.19kg/(m3·d)，出水氨氮＜1mg/L(去除率为99%)；泥龄长可能使微生物的代谢产物或其他大分子物质积累，从而抑制硝酸盐细菌的活性，导致NO2-积累而有利于短程脱氮的进行，但泥龄过长也会影响亚硝酸盐细菌的活性，从而影响对氨氮的处理效果。

A submerged membrane sequencing batch bioreactor was used to treat coke wastewater.Long running performance showed that due to the membrane interception,the nitrobacter is enriched in reactor in the interest of improving the nitrification rate;the maximum ammonia nitrogen loading can be 0.19 kg/(m3·d) with effluent ammonia nitrogen＜1 mg/L (removal rate 99%).Long sludge retention time may result in the accumulation of metabolic products and high molecular materials,and thus inhibiting activity of nitrate bacteria and causing a ccumulation of NO2-,which is beneficial to the running of short-cut denitrif ication.However,too long retention time will affect the activity of nitrite bacteria,detrimental to the treatment effect of ammonia nitrogen.

简介： 采用浸没式膜生物反应器处理焦化废水的试验结果表明：膜的截留作用可使硝化菌在反应器内富集而有利于提高系统的硝化能力，其去除氨氮的最高负荷为0.19kg/(m3·d)，出水氨氮＜1mg/L(去除率为99%)；泥龄长可能使微生物的代谢产物或其他大分子物质积累，从而抑制硝酸盐细菌的活性，导致NO2-积累而有利于短程脱氮的进行，但泥龄过长也会影响亚硝酸盐细菌的活性，从而影响对氨氮的处理效果。

It was observed by means of electronic microscope that toxinresulted in the apparent change in ultrastructure of rapeseed leafincluding the damage of chloroplast membrane,dilated,disorderedgrana lamellae;the damage of mitochondria membrance andeventually vacuolated mitochondria;the contract of cell nuclein.

电镜观察发现：菌核菌产生的毒素引起油菜叶片超显微结构显著变化，主要包括叶绿体膜破坏，基质片层肿胀，排列紊乱，形成空腔，最终叶绿体解体；线粒体膜破坏，最后空泡化；细胞核质凝缩。

With the loss of spleen function, antibody formation, opsonization and alternate complement production are all affected, producing an increased risk of infections, especially to encapsulated organisms (pneumococci, meningococci, salmonella).

随着脾脏功能、抗体形成、补体调理作用和交替产生作用的失去，机体感染几率增加，特别是对有夹膜的生物如肺炎球菌、脑膜炎球菌、沙门氏菌。

The characterization of the fermental Toxigenic Pasteurella multocida (Pm 13-1) is studied according to the vaccine standard.

为检验该培养方法培养出的T+Pm是否符合对T+Pm制苗用菌液的生物学特性鉴定标准，分别取样作培养特性检验、菌体形态检验、糖发酵实验、T+Pm毒素产生的检测及荚膜型鉴定。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。